Method for simultaneously determining concentrations of three kinds of vitamin B12 in serum

Abstract

The invention relates to a method for simultaneously determining the concentrations of three kinds of vitamin B12 in serum, the three kinds of vitamin B12 are cyanocobalamin, mecobalamin and cobalamin (5'-deoxycobalamin and coenzyme B12) respectively, a two-dimensional liquid chromatography system and a column switching technology are combined, and a serum sample to be detected after protein removal is subjected to machine detection. According to the method, a two-dimensional liquid chromatography system and a simple pretreatment (deproteinization) method are adopted, errors caused by the operation process are reduced, and the detection flux is improved. The same analysis method can be used for simultaneous determination of three vitamins B12 in serum, standard curves can be established at the same time, and the substances to be detected do not interfere with each other. The time of alternately balancing the system by different methods during sample analysis is greatly reduced.

Description

technical field [0001] The invention relates to the technical field of drug detection, in particular to a method for simultaneously measuring the concentrations of three vitamin B12 in serum. Background technique [0002] Vitamin B12, also known as cobalamin (Cobalamin), is a general term for a class of cobalt-containing corrinoids. It is the only vitamin containing metal elements and the latest to be discovered among the B vitamins. Vitamin B12 is a polycyclic compound containing trivalent cobalt. Four reduced pyrrole rings are connected together to form a corrin macrocycle (similar to porphyrin), which is the core of the vitamin B12 molecule, so it contains this Compounds with this ring are called corrinoids. Vitamin B12 is the most complex small molecular substance biosynthesized in nature. It participates in the manufacture of bone marrow red blood cells to prevent pernicious anemia; it participates in human cell metabolism and affects the synthesis and regulation of DN...

AI Technical Summary

Problems solved by technology

Because the antibody used in the immunoassay method cannot distinguish the different forms of cobalamin, it cannot distinguish and measure different forms of cobalamin, and the detection accuracy is not high; and although the detection accuracy of high-performance liquid chromatography is greatly improved, the sensitivity is up to It does not meet the detection requirements of serum cobalamin; the high-performance liquid chromatography-mass spectrometry of cobalamin reported so far only detects cyanocobalamin, and the sensitivity does not meet the requirements, and there is no method for simultaneous determination of three vitamin B12 in serum A high performance liquid chromatography-mass spectrometry method was reported

Images