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Establishment method of chronic ocular hypertension and long ocular axis animal model, model and application

A technology of animal models and establishment methods, applied in veterinary instruments, veterinary surgery, medical science, etc., can solve the problems of long model construction period, high economic cost, lack of simple and effective animal models, etc.

Inactive Publication Date: 2021-07-20
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the common surgical construction methods and characteristics of chronic high intraocular pressure animal models of glaucoma are: (1) the method of blocking the trabecular meshwork by anterior chamber injection, such as injection of microbeads, chemically cross-linked hydrogel, chondroitin sulfate, carbomer, For sodium hyaluronate, methylcellulose, etc., the anterior chamber injection method has the characteristics that the intraocular pressure increases significantly, but the injections, especially sodium hyaluronate and carbomer, have a short maintenance time and may require repeated injections, and Since the operation involves intraocular injection, it may cause intraocular inflammatory reactions, and the possible toxic effects of the injection on the retina may interfere with the test results; Photocoagulation has the characteristics of high requirements for surgical equipment, and the success rate is related to laser intensity and repeated photocoagulation times; (3) drug induction method: such as intravitreal injection of corticosteroids, tumor necrosis factor β (TGF-β) etc., this type of method has the characteristics of: intravitreal injections cause retinal toxic reactions, and intraocular operations have the characteristics of causing the risk of endophthalmitis; (4) transgenic animals: such as with Gpnmb R150X and Tyrp 1isa Related DBA / 2J mice, and Tyr 437 Related Tg-MYOC mice, B6.Sh3pxd2b related to Sh3pxd2b nee Mice, etc. also have the characteristics of chronic high intraocular pressure. This kind of animal has the characteristics of long model construction period and high economic cost
The current animal models of glaucoma with high intraocular pressure focus on the simulation and research of retinal damage and changes in the number and function of retinal ganglion cells caused by elevated intraocular pressure. There is still a lack of simple and effective animal models that can be applied to glaucoma with myopia

Method used

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  • Establishment method of chronic ocular hypertension and long ocular axis animal model, model and application
  • Establishment method of chronic ocular hypertension and long ocular axis animal model, model and application
  • Establishment method of chronic ocular hypertension and long ocular axis animal model, model and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] This example is used to establish a chronic high eye pressure combined with long eye shaft animal model.

[0054] Preparation of model animals:

[0055] 40 SD healthy male rats were selected from 7-8 weeks, randomly divided into two groups, 20 each group, respectively as a model group and control group, respectively. In the temperature of 20-26 ° C, the relative humidity is 60%, and it is raised in an environment freely eating drinking water. 3 days before surgery, 8-10 o'clock per day, measured, was recorded as baseline intraocular pressure in the previous day, after which intraocular pressure detection was performed at this time period.

[0056] Establishment of animal models:

[0057] The establishment of animal models figure 1 As shown, mainly includes the following steps:

[0058] S10, anesthesia for model animals: 2% pentobarbital sodium is injected with 2% pentobarbital sodium in the abdominal cavity of the model group and the control group to perform a systemic anes...

Embodiment 2

[0069] The present embodiment uses the high eye pressure of the detection model animal and the change in the ball thereof.

[0070] 1 day, 1 week, 2 weeks, 4 weeks, February and June, and analyzed the changes of the intra-pressure meter in different time periods, and analyzed the eye pressure of the rat. Such as Figure 5 Indicated. by Figure 5 It can be seen that after the rat is sutured by a ring angle, the eye pressure suddenly increases when the suture is ligated. At this time, if Figure 4 The B is shown in the B figure, the corneal oxygen in the rats is white, corneal edema, and pupil. The rats under normal awake state were 10-13 mmHg, and the intraocular pressure of 5 minutes after the normal control group in this example was 12.2 ± 1.5 mmHg. The peak of the eye pressure measurement of the model group can reach 98 mmHg, and 5 minutes of intraoperative pressure dropped to 61.4 ± 10.4 mmHg. And gradually decline over time. The intraocular pressure obtained was 25.1 ± 3.6 mmHg 1...

Embodiment 3

[0074] This embodiment uses the long eye axis of the detection model animal and the change of the scleral tissue.

[0075]The model group rattles and control group rats were taken at 3 performed in the 4 weeks, and the complete eyeballs were taken out, and the complete eyeball was tagged. After the decada sclera (2mm after corneal), 2.5% glutaraldehyde fixation was fixed, prepared The electron microscope specimens use transmissive electron microstructures for ultrastructural observation of scleral collagen fibers. Such as Figure 12 As shown, the collagen fiber structure of the comparative control group in the model group ratio increased.

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Abstract

The invention discloses an establishment method for a chronic ocular hypertension and long ocular axis animal model. The establishment method comprises the following steps of S10, anesthetizing a model animal; S20, using a non-absorbable suture line for intermittently suturing a conjunctiva and a superficial sclera for a circle along the outer periphery of a cornea at a set distance; S30, tightening the suture line, and knotting and fixing; and S40, measuring intraocular pressure of the model animal within 5 minutes after knotting, and if the intraocular pressure is larger than 60 mmHg (1 mmHg = 0.133 kPa), the chronic ocular hypertension and long ocular axis animal model is obtained. The intraocular pressure of the model animal can be increased, ocular axis can be increased, and the method has the advantages of long intraocular pressure maintaining time, few ocular complications, simple operation and high operation success rate. In addition, the invention further discloses the chronic ocular hypertension and long ocular axis animal model and application thereof in research of glaucoma and myopia diseases.

Description

Technical field [0001] The present invention relates to an animal disease model, and more particularly to a method of establishing a chronic high-eye pressure combined with a long eye shaft animal model. The present invention also relates to a chronic high-eye pressure combined with long eye shaft animal model and a chronic high-eye pressure combined with long eye shaft animal model in studying the study of glaucoma combined with myopia. Background technique [0002] Glaguatic eye is a class of irreversible blind eye diseases that are mainly manifested by optic nerve injury and vision defects. The essence is irreversible death in retinal ganglion cells (RGCs). The main risk factors are pathological intraocular pressure ( Intraocular Pressure, IOP) rises. At present, the main treatment solution for glaucoma is to reduce IOP through drugs or surgery methods. The main clinical features of pathological myopia are characteristics that the decummission is greater than -6d and the eye a...

Claims

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Application Information

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IPC IPC(8): A01K67/02A61D1/00
CPCA01K67/02A61D1/00A01K2227/105A01K2267/03
Inventor 夏明芳夏晓波李海波姚飞张恩东胡涂
Owner CENT SOUTH UNIV
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