Infectious hematopoietic necrosis vaccine and method for amplifying infectious hematopoietic necrosis virus on epithelioma papulosum cyprini

A technology for hematopoietic organ necrosis and epithelial cells, applied in the biological field, can solve problems such as IHNV-free in vitro proliferation solutions
CN113144185APending Publication Date: 2021-07-23HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
Publication Date
2021-07-23

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Abstract

The invention discloses an infectious hematopoietic necrosis vaccine and a method for amplifying infectious hematopoietic necrosis virus (IHNV) on epithelioma papulosum cyprini (EPC). The invention provides a method for preparing an infectious hematopoietic necrosis vaccine, and the method comprises the following steps: 1) inoculating IHNV to EPC according to an MOI value of 0.0001, carrying out culturing, and collecting supernatant, thereby obtaining proliferated IHNV; and 2) preparing the infectious hematopoietic necrosis vaccine by using the proliferated IHNV. Experiments in the invention prove that when the IHNV is inoculated to the EPC with the concentration of MOI = 0.0001, the required virus harvest time is short, and the virus titer is high and stable. The IHNV is amplified on a large scale based on the proliferation scheme, and an inactivated vaccine is prepared from BPL and formaldehyde. Results show that the vaccine has a good immune effect on Oncorhynchus mykiss, and the relative immune protection efficiency was up to 84%.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a vaccine for infectious hematopoietic organ necrosis and a method for amplifying the virus on the epithelial cells of the crocodile. Background technique

[0002] Infectious hematopoietic necrosis virus (IHNV) belongs to Rhabdoviridae (Rhabdoviridae), Nora rhabdovirus (Novirhabdovirus), is a single-stranded negative-sense RNA virus, is infectious hematopoietic necrosis (Infectious hematopoietic necrosis virus, hematopoietic necrosis, IHN).

[0003] Previous studies have found that in the process of subculture of IHNV, when the inoculation dose is too large, there will be a large amount of IHNV defective virus packaged wrongly, and the defective virus does not have the ability to reproduce. With the increase of the number of passages, the defective virus will accumulate rapidly. Ultimately, IHNV cannot continue to be passed on in vitro; when the inoculation dose is too s...

Claims

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