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Citrobacter freundii AS11 and application thereof in sewage treatment

A Freund's citric acid, AS11 technology, applied in biological water/sewage treatment, water/sludge/sewage treatment, animal husbandry wastewater treatment, etc., can solve the problem of increased investment and operation cost, limited denitrification application, harsh growth conditions, etc. To achieve the effect of saving processing space and cost, simplifying the denitrification process, and wide pH adaptability

Active Publication Date: 2021-07-23
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional biological denitrification process includes two processes of aerobic autotrophic nitrification and anaerobic heterotrophic denitrification, but it has the following disadvantages: (1) Autotrophic nitrifying bacteria grow slowly, have poor environmental adaptability, weak impact load resistance, and are easily destroyed by high Concentration of ammonia nitrogen and nitrite nitrogen inhibition
(2) The two reactions of nitrification and denitrification cannot be unified in time and space, which increases investment and operation costs
However, the suitable growth conditions of most of the strains isolated at present are relatively harsh, and the denitrification application for some special types of wastewater (such as acid-base wastewater, high-salt wastewater, and high-ammonia nitrogen wastewater) is limited. The removal of high-concentration ammonia nitrogen and the high removal rate of Citrobacter freundii (Citrobacter freundii) for swine wastewater COD and ammonia nitrogen have not been reported

Method used

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  • Citrobacter freundii AS11 and application thereof in sewage treatment
  • Citrobacter freundii AS11 and application thereof in sewage treatment
  • Citrobacter freundii AS11 and application thereof in sewage treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Isolation and identification of strains:

[0024] Enrichment culture: The sample is the aerobic activated sludge collected by Cao Xianhe from a sewage treatment plant in Beijing in April 2020. Take 20mL of fully mixed activated sludge and suspend it in 180mL0.2% sodium chloride solution. Take 5mL The suspension was placed in a 250mL Erlenmeyer flask filled with 100mL heterotrophic nitrification medium, and acclimatization and enrichment culture was carried out at 30°C and 180rpm. Acclimatization takes 48h as a cycle. After each cycle, take 10 mL of the enrichment solution and add it to a new 100 mL heterotrophic nitrification medium to continue the enrichment culture, and thus continue the enrichment culture for 5 cycles. During this period, the removal of ammonia nitrogen in the culture medium was detected.

[0025] Take the culture solution after enrichment for 5 generations, and use sterile water according to 10 -3 -10 -7 Different proportions of gradient dilutio...

Embodiment 2

[0036] Growth and heterotrophic nitrification characteristics of strain AS11:

[0037] After the bacterial strain AS11 was activated and cultured in the LB liquid test tube for 24 hours, a certain amount of bacterial solution was drawn according to the inoculum amount (v / v) of 2%, and the bacterial cells were collected after centrifugation at 4000 rpm for 5 minutes, washed with sterile water and inoculated in the container. In a 150mL Erlenmeyer flask with 50mL of sterilized heterotrophic nitrification medium (initial ammonia nitrogen concentration 100mg / L, C / N=20), cultivate it in a shaker at 30°C and 180rpm for 72h, during which time samples were taken every 4h to measure OD600 value, and the sample was centrifuged at 10000rpm for 10min to remove the bacteria, and then the supernatant was taken to detect COD and NH in the water sample4 -N, NO 2 -N, NO 3 -N, TN content.

[0038] The results are as follows and figure 1 shown. The strain began to grow 8 hours after inoculat...

Embodiment 3

[0043] The effect of pH on the growth and denitrification of strain AS11:

[0044] Adjust the pH of the heterotrophic nitrification medium to be 3, 4, 5, 7, 8.5, 9.5, 10.5, 11.5 respectively, after activating the bacterial strain AS11 according to the conventional method, draw a certain amount of The bacteria solution was centrifuged at 4000rpm for 5min to collect the bacteria, washed with sterile water, inoculated in the heterotrophic nitrification medium after adjusting the pH, cultured in a shaker at 30°C and 180rpm for 72h, and tested the final pH value under each pH condition. OD600 value, and the sample was centrifuged at 10000rpm for 10min to remove the bacteria, and then the supernatant was taken to detect COD and NH in the water sample 4 -N content.

[0045] The results are as follows and figure 2 shown. Strain AS11 can grow well and carry out heterotrophic nitrification in the pH range of 5-10.5, and the removal rate of ammonia nitrogen is between 90%-93%, and th...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to the technical field of microorganism wastewater treatment. The invention discloses a strain of Citrobacter freundii AS11 with a preservation number of CGMCC No.21283. The strain is preserved in the China General Microbiological Culture Collection Center on December 2, 2020, the China General Microbiological Culture Collection Center is called CGMCC for short, the address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.21283. The strain can realize synchronous removal of ammonia nitrogen, total nitrogen and COD under an aerobic condition.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, in particular to the technical field of microbial wastewater treatment. Background technique [0002] The main pollutants that have been detected to exceed the standard in my country's fresh water resources (including rivers, lakes, large-scale water conservancy projects and other surface water and groundwater) include chemical oxygen demand (COD), ammonia nitrogen (NH 4 -N), total nitrogen (TN) and total phosphorus (TP), etc. Among them, nitrogen has a variety of pollutants and is difficult to remove. When high concentrations of nitrogenous pollutants such as ammonia and nitrite are discharged into the environment, on the one hand, it will cause eutrophication of the water body, and on the other hand, it will cause eutrophication. On the one hand, it will also produce biological toxicity to a variety of organisms, so the theory and technology of denitrification have always been a researc...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F103/20C02F101/16
CPCC12N1/20C02F3/34C02F2103/20C02F2101/16
Inventor 李少杰曹先贺孙宪昀张振颖胡成成
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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