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Bacteroides dorei probiotics and application thereof in preparation of drugs for treating or preventing influenza

A technology of Bacteroides multinerii and microorganisms, applied in the field of microorganisms, can solve problems such as reducing the severity of diseases, and achieve the effects of prolonging the survival rate, protecting the length of the colon, and having a strong ability to decompose quercetin

Active Publication Date: 2021-07-23
ICDC CHINA CDC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

While alterations in fecal microbiota are associated with fecal levels of SARS-CoV-2 and COVID-19 severity, strategies to alter gut microbiota may reduce disease severity

Method used

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  • Bacteroides dorei probiotics and application thereof in preparation of drugs for treating or preventing influenza
  • Bacteroides dorei probiotics and application thereof in preparation of drugs for treating or preventing influenza
  • Bacteroides dorei probiotics and application thereof in preparation of drugs for treating or preventing influenza

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1. Isolation, screening and identification of Bacteroides multani

[0054] 1.1B. Separation of dorei XR2020

[0055] 1) Prepare brain heart infusion (BHI) agar medium, after 121 ° C, 15 min autoclaving, cool to 50 ° C, shake gently, then add 5% defibrinated sheep blood, mix well and pour into a petri dish;

[0056] 2) Take the 10 diluted by gradient -3 100ul of the stool sample is spread on the culture medium of 1);

[0057] 3) At 37°C, 0.5% CO 2 Cultivate in the environment for 48h;

[0058] 4) Pick a single moist, round and raised off-white colony for transfer.

[0059] 1.2 Strain preservation

[0060] In this laboratory, BHI medium containing 30% glycerol is used as a bacteria preservation solution for cryopreservation of strains. The method is as follows:

[0061] 1) Sterilize the 2mL sterile tube by autoclaving at 121°C for 15 minutes for use;

[0062] 2) After the bacteria are continuously transferred on the BHI medium for 3 times, add 2ml of steril...

Embodiment 2

[0077] Example 2, Research on the anti-influenza function of B.dorei XR2020

[0078] 2.1 Research methods:

[0079] 64 6-8 week female SPF level C57BL / 6J mice adapted for 7 days, weighing 17-18 grams, were randomly divided into four groups, Control (blank control, not infected with influenza virus), Model (model group, infected with influenza virus 100 μL 1×PBS was given by intragastric administration for 7 days), Oseltamivir (oseltamivir group, 100 μL oseltamivir was given by intragastric administration for 7 days after influenza virus infection) and B. In the Bacteroides group, 100 μL of Bacteroides multidrevii was given by intragastric administration the next day after being infected with influenza virus for 7 days), with 16 rats in each group. Prepare 3% chloral hydrate and anesthetize mice by intraperitoneal injection of 0.12ml chloral hydrate solution per 10g body weight. After the mice in the three infection groups (model group, oseltamivir group and Bacteroides group...

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Abstract

The invention discloses a probiotic candidate strain. The preservation number of the probiotic candidate strain is CGMCC NO.21251, the preservation date is November 26, 2020, the preservation classification name of the probiotic candidate strain is Bacteroides doreiXR2020 (B. dorei XR2020), and the preservation unit is China General Microbiological Culture Collection Center (CGMCC). The strain is harmless to animals and has a remarkable effect of improving clinical symptoms of influenza A. The invention further discloses application of the strain and thallus components thereof in preparation of drugs for preventing and treating influenza A.

Description

technical field [0001] The invention relates to a probiotic candidate strain and the application of bacterial body components thereof, belonging to the field of microorganisms. Background technique [0002] Influenza viruses include three types: A, B, and C. Type A is the most likely to cause epidemics, followed by Type B, and Type C rarely causes epidemics. According to the antigenicity of the outer membrane hemagglutinin (HA) and neuraminidase (NA) proteins of virus particles, influenza A virus can be divided into 16 H subtypes (H1-H16) and 9 N subtypes ( N1-N9), because the nucleotide sequences encoding HA and (or) NA are prone to mutations, resulting in changes in the antigenic epitopes of HA and (or) NA, this antigenic change makes the original specific immunity of the population Force failure, so influenza A virus often causes large-scale or even worldwide influenza epidemic. The Spanish H1N1 flu pandemic of 1918 killed an estimated 50 million people. In 2009, H1N1 ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/741A61P31/16A61P11/00A23L33/135C12R1/01
CPCA61K35/741A61P31/16A61P11/00A23L33/135A23V2002/00A23V2200/314Y02A50/30
Inventor 徐建国任志鸿卢珊宋利琼肖玉春李先平黄元铭
Owner ICDC CHINA CDC
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