A kit for detecting Drosophila spotted wing based on terrestrial environment DNA and its application

A spotted-winged Drosophila and a kit technology, applied in the field of agricultural biology, can solve the problems that the identification and monitoring are difficult to eliminate the influence of interfering factors, the technicians cannot make very objective judgments, the detection object is single, etc., and the detection efficiency and timeliness are achieved. The effect of good performance and simple detection process
CN113151511BActive Publication Date: 2022-03-01HUNAN UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HUNAN UNIV OF SCI & TECH
Publication Date
2022-03-01

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Abstract

The invention discloses a kit product suitable for quantitative detection of spotted wing Drosophila pest damage based on terrestrial environment DNA. The kit includes Drosophila spotted wing upstream primer (BCGY4‑F), Drosophila spotted wing downstream primer (BCGY4‑R), Taqman fluorescent probe and qPCR Mix four reagents. This kit can be used in conjunction with other commercially available products, such as Carmel peristaltic pumps, Millipore polycarbonate erosion membranes, qPCR8-connected tubes, etc., to quantify the results on fruits and vegetables that have been mixed with Drosophila spotted wing for a period of time. The amount of DNA left by Drosophila spotted wing was detected. Steps for use: 1. Take fruits and vegetables and rinse; 2. Etch the filter membrane to filter the rinse solution; 3. Cut the filter membrane to collect eDNA; 4. Use eDNA as a template to perform qPCR reaction. This product can be used in the qualitative detection of pests in orchards, with high specificity and good timeliness; it can also quantitatively detect the density of pests in the environment, which is conducive to reflecting the seriousness of pest damage and predicting the occurrence of pest damage in the next year. At the same time, this product also has potential value in customs detection of invasive alien species and ecological population analysis.
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Description

technical field

[0001] The invention relates to the field of agricultural biotechnology, in particular to a kit for detecting Drosophila spottedwings based on terrestrial environment DNA and an application thereof. Background technique

[0002] Spotted Drosophila ( Drosophila suzukii ), also known as Drosophila suzuki, is an important agricultural pest in the Drosophila family. The ovipositor of the female is hard and serrated, and can lay eggs directly in ripe or soon-to-mature grapes, strawberries, blueberries, tomatoes, cherries and other fruits and vegetables. The area around the food point quickly begins to rot, and causes secondary infection of fungi and bacteria, which accelerates the rot of the fruit. Once an insect infestation occurs, it will cause serious losses to the orchard. Qualitative detection of the presence or absence of Drosophila spotted wing and quantitative detection of the density of Drosophila spotted wing will have important indication significanc...

Claims

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