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A kind of medical adhesive and preparation method thereof

A technology of adhesives and catalysts, applied in pharmaceutical formulations, surgical adhesives, applications, etc., can solve problems such as insufficient mechanical strength, easy coagulation, secondary tissue damage, etc., to ensure long-term stability and non-toxic degradation products , Conducive to the effect of clinical promotion

Active Publication Date: 2022-05-03
THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But all have certain limitations. Fibrin adhesives are extracted from human plasma, so the cost is more expensive than synthetic materials; and the spread of potential diseases related to blood products has brought serious safety risks; more Most importantly, fibrin adhesives suffer from poor bond strength and insufficient mechanical strength in a moist physiological environment filled with blood and interstitial fluid.
The high toxicity of aldehyde-containing products severely limits the application of related adhesive products in the human body
The polymerization heat generated during the rapid polymerization of cyanoacrylates is easy to cause secondary damage to the tissue, the elasticity and flexibility after curing are poor, and the storage period is short and easy to solidify. These shortcomings limit the use of cyanoacrylate adhesives. Wide application in clinical medicine, especially in the repair of soft tissues in the body

Method used

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  • A kind of medical adhesive and preparation method thereof
  • A kind of medical adhesive and preparation method thereof
  • A kind of medical adhesive and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] This embodiment is a preparation method of a medical adhesive, the preparation method comprising the following steps:

[0038] (a) According to the volume ratio of 1:1, add the 2-chloroethylamine solution with a concentration of 1M into the gelatin aqueous solution with a concentration of 0.1g / ml and stir for 30min, then adjust the pH value to 10.5 and react at 55°C for 24h , to obtain gelatin-ethylamine;

[0039] (b) Use PBS buffer to prepare a gelatin-ethylamine solution with a concentration of 0.6g / ml, and adjust the pH to 4.2, then add EDC to a final concentration of 0.005g / ml, and NHS to a final concentration of 0.003g / ml , 3,4-dihydroxybenzoic acid to a final concentration of 0.02g / ml, and then reacted at 37°C for 24 hours, and then dialyzed the reaction solution in deionized water for two days and freeze-dried to obtain gelatin-3,4- Dihydroxybenzoic acid;

[0040] (c) Mix gelatin-3,4-dihydroxybenzoic acid and TG enzyme according to the mass ratio of gelatin-3,4...

Embodiment 2

[0044] This embodiment is a preparation method of a medical adhesive, the preparation method comprising the following steps:

[0045] (a) According to the volume ratio of 1:1.2, add the 2-chloroethylamine solution with a concentration of 1.2M into the whey protein aqueous solution with a concentration of 0.15g / ml and stir for 30min, then adjust the pH value to 11.0 and heat at 52°C Down reaction 26h, obtain whey protein-ethylamine;

[0046] (b) Prepare a whey protein-ethylamine solution with a concentration of 6g / ml in PBS buffer, adjust the pH to 5.6, add EDC to a final concentration of 0.012g / ml, and NHS to a final concentration of 0.016g / ml ml, 3,4-dihydroxyphenylacetic acid to a final concentration of 0.09g / ml, and then reacted at 35°C for 26 hours, and then dialyzed the reaction solution in deionized water for two days and freeze-dried to obtain whey protein-3 ,4-dihydroxyphenylacetic acid;

[0047] (c) Mix whey protein-3,4-dihydroxyphenylacetic acid and TG enzyme accor...

Embodiment 3

[0050] This embodiment is a preparation method of a medical adhesive, the preparation method comprising the following steps:

[0051] (a) According to the volume ratio of 1:0.8, add 2-chloroethylamine solution with a concentration of 0.8M into an aqueous gelatin solution with a concentration of 0.05g / ml and stir for 30min, then adjust the pH value to 12.0 and react at 58°C 22h, obtain gelatin-ethylamine;

[0052] (b) Use PBS buffer to prepare a gelatin-ethylamine solution with a concentration of 0.5g / ml, and adjust the pH value to 4.6, then add EDC to a final concentration of 0.01g / ml, and NHS to a final concentration of 0.02g / ml , 3,4-dihydroxyphenylpropionic acid to a final concentration of 0.01g / ml, and then reacted at 39°C for 22 hours, and then dialyzed the reaction solution in deionized water for two days and freeze-dried to obtain gelatin-3,4 - dihydroxyphenylpropionic acid;

[0053] (c) Mix gelatin-3,4-dihydroxyphenylpropionic acid and TG enzyme according to the mass r...

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Abstract

The invention discloses a medical adhesive and a preparation method thereof. The preparation method comprises reacting 2-chloroethylamine with gelatin to obtain gelatin-ethylamine; Hydroxybenzene carboxylic acid is reacted to obtain gelatin-o-dihydroxybenzene derivatives; gelatin-o-dihydroxybenzene derivatives are mixed with TG enzyme, and then metal ion solution is added for cross-linking reaction to obtain the medical adhesive; the The adhesive still has good adhesion in a humid environment and can be quickly cured, and has good stability. In addition, the required raw materials are simple, easy to obtain, and economical, and the cost of the final product can be effectively controlled, which is conducive to clinical promotion; at the same time, the cell Toxicity tests, rabbit carotid artery wrapping, hemolysis tests and the like have confirmed that the adhesive prepared by the invention is safe and non-toxic, and the degradation products are non-toxic.

Description

technical field [0001] The invention relates to the technical field of adhesives, in particular to a medical adhesive and a preparation method thereof. Background technique [0002] Uncontrolled bleeding after trauma or surgery is a leading cause of mortality worldwide, and hemorrhage and oozing after vascular anastomoses have plagued cardiologists. Especially the difficult-to-control bleeding in large vessel anastomosis has led to high complications and high mortality in patients. Currently, surgical suture is the only clinical method for sealing aortic rupture, but the bleeding after suturing is difficult to control, which can only be solved by compression to stop bleeding in the past. [0003] With the latest developments in material science, many experimental chemical reagents have been tested for surgical hemostasis, and various medical adhesives, such as fibrin glue, albumin-glutaraldehyde adhesive mixture and cyanoacrylate, etc. Surgical procedures are facilitated. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L24/10A61L24/00C08H1/00
CPCA61L24/104A61L24/108A61L24/001A61L24/0042C08H1/00A61L2400/04C08L89/00
Inventor 范长江王国庆王建勋刘高利
Owner THE AFFILIATED HOSPITAL OF QINGDAO UNIV