Urease reinforcement liquid for slope protection and preparation and construction method thereof
A technology of strengthening liquid and urease, applied in the directions of biochemical equipment and methods, chemical instruments and methods, enzymes, etc., can solve a large number of labor costs and material costs, water and soil loss engineering accidents, environmental damage and other problems, to increase strength, increase Compression resistance, the effect of improving uniformity
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specific Embodiment approach 1
[0035] Embodiment 1: This implementation discloses a urease reinforcement solution for slope reinforcement, including a soybean urease solution, a treatment solution, skimmed milk powder and sodium alginate solution. The urease activity of the soybean urease solution is in the range of 3.5-5 mmol / min, and the treatment liquid is 0.75-1.5 mol / L urea-calcium acetate mixed solution. Skim milk powder is added to soybean urease solution to prepare enzyme liquid, and the concentrations of urease and skim milk powder in the enzyme liquid are kept at 2-4 g / L and 3-5 g / L respectively. Preferably, it is 4mmol / min active soybean urease solution, 1mol / L treatment solution, 3g / L urease and 4g / L skimmed milk powder.
specific Embodiment approach 2
[0036] Embodiment 2: This embodiment is a specific description of Embodiment 1. The concentration of the sodium alginate solution is 1.2 to 1.5 times the concentration of the enzyme solution. Preferably, the concentration of the sodium alginate solution is 1.25 times the concentration of the enzyme solution.
specific Embodiment approach 3
[0037] Specific embodiment three: This specific embodiment discloses a method for preparing a urease reinforcement solution for slope reinforcement according to specific mode one, including:
[0038] The preparation method of the soybean urease solution: take the soybeans and bake them in an oven at 40-50 DEG C for 6-7 hours, and put the dried soybeans into a pulverizer to pulverize them. In order to ensure the quality of soybean crushing, it should be crushed in small quantities and in multiple batches. The crushed soybeans should be stored in a low-temperature and dry environment for later use, and then add deionized water to the soybean powder to prepare a 15-30g / L soybean powder solution , after fully stirring the soybean powder solution with a magnetic stirrer, let it stand for 2 to 3 hours, then filter the upper layer of the soybean powder solution after centrifugation through gauze and pour it into a centrifuge tube at 3000 to 4000r / min, 4 to 6°C Centrifuge for 15 to 20...
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