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Full-automatic shallow tray culture tray type solid fermentation device and control method

A solid fermentation device and culture tray technology, applied in solid phase fermentation bioreactors, biochemical cleaning devices, biochemical equipment and methods, etc., can solve the problems of occupying cultivation space, unfavorable industrial scale-up, fermentation failure, etc.

Pending Publication Date: 2021-07-30
山西大地生态环境技术研究院有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of the device is that the condensed water in the temperature control layer will drip into the culture medium of the next layer during the fermentation process, and the local humidity is too high, resulting in fermentation failure. In addition, the temperature control method at the bottom of the culture layer occupies the cultivation space, which is not conducive to industrial scale-up.

Method used

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  • Full-automatic shallow tray culture tray type solid fermentation device and control method
  • Full-automatic shallow tray culture tray type solid fermentation device and control method

Examples

Experimental program
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Effect test

Embodiment example 1

[0069] Use of Streptomyces avermitilis in the present invention.

[0070] Spread the prepared solid medium on a shallow plate, keep the thickness at about 5cm, put it on a sterilization protection bag and put it in a pressure cooker for sterilization, after cooling, send it to the ultra-clean bench to inoculate the liquid seeds of Streptomyces avermitilis Move it into the solid incubator, turn on the air, temperature, and humidity control systems in turn, set the cultivation parameters on the controller and set the control parameter state to the automatic cultivation state, and obtain the Streptomyces avermitilis spore product after continuous cultivation for 5-7 days. The amount of spores harvested is twice that of ordinary incubators.

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PUM

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Abstract

The invention relates to the technical field of biopesticide on-line expanding culture devices, in particular to a full-automatic shallow tray culture tray type solid fermentation device and a control method. The device includes: an air compressor, an incubator body, a shallow culture tray and a PLC, and a humidity control system, a circulating temperature control system, a detection probe and an air mass flow meter which are connected with the PLC; wherein a detection probe and a plurality of shallow culture trays are arranged in the incubator body; the air compressor is connected with the incubator body through the air mass flow meter and the humidity control system in sequence; and the circulating temperature control system is connected with the incubator body. Technical parameters in the fermentation process are accurately controlled by adopting the dissolved oxygen electrode, the humidity electrode and the temperature electrode, the fermentation efficiency is high; meanwhile, the microbiological contamination rate is reduced by adopting jacket circulating water temperature control, 25-degree angle between the tray and the bottom plate of the incubator, external humidification design and the like, and the fermentation yield is improved.

Description

technical field [0001] The invention relates to the technical field of on-line expansion cultivation devices for biological pesticides, in particular to a full-automatic shallow-disc culture-disc-type solid fermentation device and a control method. Background technique [0002] Biopesticides mainly refer to the use of microorganisms and their metabolites to prevent and control crop diseases and insect pests. It is an important means of biological control. It has strong biological selectivity, is harmless to crops, humans and animals, has a direct killing effect on pathogens and nematodes, and has no pesticide residue after application. The main components of biological pesticides are bacteria and their metabolites. Such as Bacillus, actinomycetes, gibberellins and so on. [0003] my country is a big crop producing country, and a large amount of chemical pesticides are used for the prevention and control of crop diseases and insect pests. However, a large amount of chemical...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12M1/36C12M1/38C12M1/16C12M1/00
CPCC12M21/16C12M23/04C12M41/22C12M41/34
Inventor 杨成立赵彭年李洋彭湃杨洋李婷婷
Owner 山西大地生态环境技术研究院有限公司