Whole-blood circulating tumor cell cascade sorting device and method based on fluorescence activated cell sorting technology

Abstract

The invention discloses a device for cascade sorting of whole blood circulating tumor cells based on a fluorescence activated cell sorting technology. The device comprises a primary sorting pipeline and a plurality of secondary sorting pipelines, wherein the primary sorting pipeline comprises a whole blood sample inlet, a cell sorting area and a waste liquid sample outlet; the secondary sorting pipeline comprises a diluent sample inlet, a cell sorting area, a cell dilution area, a CTCs sample outlet and a waste liquid sample outlet; and the cell sorting area comprises a fluorescence detection area and a pressure control system. The device is easy and convenient to operate, all that is needed is to inject a certain volume of whole blood into the whole blood sample injection port, and the whole blood injection port is matched with sample injection of a diluent, a fluorescence detection device, a sorting device, an injection pump and the like, so non-specific cells such as red blood cells, white blood cells and blood platelets in a blood sample can be removed after a period of time, and finally high-purity circulating tumor cells are obtained.

Description

technical field [0001] The invention belongs to the technical field of medical testing, and in particular relates to a device and method for cascade sorting of whole blood circulation tumor cells based on fluorescence activated cell sorting technology. Background technique [0002] Circulating tumor cells (Circulating Tumor Cells, CTCs) refer to the tumor cells that are detached from the entity of the primary tumor body and free in the whole blood. CTCs carry a large amount of biological information of source tumor cells, which is of great significance to the monitoring of gene mutations, drug resistance changes, and epigenetic variations of primary tumors in clinical practice. Moreover, the analysis of CTCs is also of great significance for monitoring the prognosis of patients and judging the recurrence of tumors. [0003] However, it is difficult to efficiently isolate these CTCs with important biological information from human peripheral blood. Various technologies have...

AI Technical Summary

Problems solved by technology

At present, only flow cytometer has similar functions, but the equipment of flow cytometer is expensive, the analysis cost is too high, and a single analysis requires a large number of blood samples

Therefore, the sorting and analysis of CTCs remains challenging

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