Whole-blood circulating tumor cell cascade sorting device and method based on fluorescence activated cell sorting technology

A tumor cell and fluorescence activation technology, applied in the field of medical testing, can solve the problems of high analysis cost, large quantity, and expensive flow cytometer equipment, and achieve the effects of shortening analysis time, low cost, and reducing pollution risks

Active Publication Date: 2021-07-30
NANTONG UNIVERSITY
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  • Application Information

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Problems solved by technology

At present, only flow cytometer has similar functions, but the equipment of flow cytometer is expensive, the analysis cost is too

Method used

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  • Whole-blood circulating tumor cell cascade sorting device and method based on fluorescence activated cell sorting technology
  • Whole-blood circulating tumor cell cascade sorting device and method based on fluorescence activated cell sorting technology
  • Whole-blood circulating tumor cell cascade sorting device and method based on fluorescence activated cell sorting technology

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[0033] The present invention will be further described below with reference to the accompanying drawings.

[0034] A total blood circulating tumor cell cascade selection based on fluorescence activated cell sorting techniques, including primary sorting lines and several secondary sorting lines; the secondary sorting line series N ≥ 2, Actual demand increases or reduces the level to achieve high efficiency. The material of the sorting line is a highly elastic and biocompatible high-performance polyolefin thermoplastic elastomer material, and the inner surface has hydrophobic properties.

[0035] The primary sorting line includes a whole blood oxide opening 1, a cell sorting region 4, a waste liquid exit 7; the secondary sorting line includes a diluted liquid gas-inlet 2, a cell dilute region 4, a cell dilution region 5 , CTCS outlet 6 and waste liquid exit 7; the cell sorting zone 4 includes a fluorescent detection zone 3 and a pressure control system 8;

[0036] The whole blood ox...

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Abstract

The invention discloses a device for cascade sorting of whole blood circulating tumor cells based on a fluorescence activated cell sorting technology. The device comprises a primary sorting pipeline and a plurality of secondary sorting pipelines, wherein the primary sorting pipeline comprises a whole blood sample inlet, a cell sorting area and a waste liquid sample outlet; the secondary sorting pipeline comprises a diluent sample inlet, a cell sorting area, a cell dilution area, a CTCs sample outlet and a waste liquid sample outlet; and the cell sorting area comprises a fluorescence detection area and a pressure control system. The device is easy and convenient to operate, all that is needed is to inject a certain volume of whole blood into the whole blood sample injection port, and the whole blood injection port is matched with sample injection of a diluent, a fluorescence detection device, a sorting device, an injection pump and the like, so non-specific cells such as red blood cells, white blood cells and blood platelets in a blood sample can be removed after a period of time, and finally high-purity circulating tumor cells are obtained.

Description

technical field [0001] The invention belongs to the technical field of medical testing, and in particular relates to a device and method for cascade sorting of whole blood circulation tumor cells based on fluorescence activated cell sorting technology. Background technique [0002] Circulating tumor cells (Circulating Tumor Cells, CTCs) refer to the tumor cells that are detached from the entity of the primary tumor body and free in the whole blood. CTCs carry a large amount of biological information of source tumor cells, which is of great significance to the monitoring of gene mutations, drug resistance changes, and epigenetic variations of primary tumors in clinical practice. Moreover, the analysis of CTCs is also of great significance for monitoring the prognosis of patients and judging the recurrence of tumors. [0003] However, it is difficult to efficiently isolate these CTCs with important biological information from human peripheral blood. Various technologies have...

Claims

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Application Information

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IPC IPC(8): G01N15/14G01N21/64G01N35/00G01N1/38
CPCG01N15/1434G01N21/6428G01N35/00584G01N1/38G01N2015/1006G01N2021/6439
Inventor 秦玉岭唐曲丛辉吴丽陈欢欢胡文琪毛天智
Owner NANTONG UNIVERSITY
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