Whole-blood circulating tumor cell cascade sorting device and method based on fluorescence activated cell sorting technology
A tumor cell and fluorescence activation technology, applied in the field of medical testing, can solve the problems of high analysis cost, large quantity, and expensive flow cytometer equipment, and achieve the effects of shortening analysis time, low cost, and reducing pollution risks
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[0033] The present invention will be further described below with reference to the accompanying drawings.
[0034] A total blood circulating tumor cell cascade selection based on fluorescence activated cell sorting techniques, including primary sorting lines and several secondary sorting lines; the secondary sorting line series N ≥ 2, Actual demand increases or reduces the level to achieve high efficiency. The material of the sorting line is a highly elastic and biocompatible high-performance polyolefin thermoplastic elastomer material, and the inner surface has hydrophobic properties.
[0035] The primary sorting line includes a whole blood oxide opening 1, a cell sorting region 4, a waste liquid exit 7; the secondary sorting line includes a diluted liquid gas-inlet 2, a cell dilute region 4, a cell dilution region 5 , CTCS outlet 6 and waste liquid exit 7; the cell sorting zone 4 includes a fluorescent detection zone 3 and a pressure control system 8;
[0036] The whole blood ox...
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