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Nanofiber for treating bacterial infection diabetes wound and preparation method and application thereof

A nanofiber and bacterial infection technology, which is applied in the direction of antibacterial drugs, medical preparations containing active ingredients, metabolic diseases, etc., to achieve low production costs, good biocompatibility, and good reproducibility

Pending Publication Date: 2021-08-03
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no ideal drug that can not only control drug-resistant bacterial infection in diabetic wounds but also promote wound healing. It is urgent to develop a good material for repairing diabetic wound drugs

Method used

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  • Nanofiber for treating bacterial infection diabetes wound and preparation method and application thereof
  • Nanofiber for treating bacterial infection diabetes wound and preparation method and application thereof
  • Nanofiber for treating bacterial infection diabetes wound and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053](1) Preparation of curcumin nanocrystals: 100mg of curcumin (Cur) was dissolved in 100mL of absolute ethanol, after fully dissolving, 500mL of ultrapure water was added, and left to stand for 2h, curcumin nanocrystals were precipitated, and curcumin-containing nanocrystals were obtained. crystal solution.

[0054] (2) Preparation of curcumin polydopamine nanofibers: add 500mg dopamine hydrochloride to the solution containing curcumin nanocrystals obtained in step (1), and add 3mL concentration of 1.5M Tris hydrochloride (Tris-HCl buffer solution adjusts the pH of the solution to be 8.8; seal and gently stir for 24h, then centrifuge at 12000rpm for 10min to collect curcumin polydopamine nanofibers; add 50mL ultrapure water to ultrasonically resuspend curcumin polydopamine nanofibers, centrifuge at 12000rpm for 10min, repeat 3 times; curcumin polydopamine nanofibers (PDA / CurNFs) were freeze-dried in a freeze dryer and collected for later use.

[0055] (3) Preparation of p...

Embodiment 2

[0058] (1) Preparation of curcumin nanocrystals: 100mg of curcumin (Cur) was dissolved in 300mL of absolute ethanol, after fully dissolving, 2400mL of ultrapure water was added, and left to stand for 2h, curcumin nanocrystals were precipitated, and curcumin-containing nanocrystals were obtained. crystal solution.

[0059] (2) Preparation of curcumin polydopamine nanofibers: add 1000mg dopamine hydrochloride to the solution containing curcumin nanocrystals obtained in step (1), and add 3mL concentration of 1.5M Tris hydrochloride (Tris-HCl) buffer solution to adjust the pH of the solution to 8.5; seal and gently stir for 72h, then centrifuge at 12000rpm for 10min to collect curcumin polydopamine nanofibers; add 100mL ultrapure water to ultrasonically resuspend curcumin polydopamine nanofibers, centrifuge at 12000rpm for 10min, Repeat 3 times; freeze-dry curcumin polydopamine nanofibers (PDA / CurNFs) in a freeze dryer and collect them for later use.

[0060] (3) Preparation of p...

Embodiment 3

[0063] (1) Preparation of curcumin nanocrystals: Dissolve 80mg curcumin (Cur) in 160mL absolute ethanol, add 960mL ultrapure water after fully dissolving, let stand for 2h, curcumin nanocrystals are precipitated, and curcumin-containing nanocrystals are obtained. crystal solution.

[0064] (2) Preparation of curcumin polydopamine nanofibers: add 640mg dopamine hydrochloride to the solution containing curcumin nanocrystals obtained in step (1), and add 3mL concentration of 1.5M Tris hydrochloride (Tris-HCl) buffer solution to adjust the pH of the solution to be 8.7; seal and gently stir for 36h, then centrifuge at 12000rpm for 10min to collect curcumin polydopamine nanofibers; add 80mL ultrapure water to ultrasonically resuspend curcumin polydopamine nanofibers, centrifuge at 12000rpm for 10min, Repeat 3 times; freeze-dry curcumin polydopamine nanofibers (PDA / CurNFs) in a freeze dryer and collect them for later use.

[0065] (3) Preparation of polydopamine nanotubes: Take 50g ...

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Abstract

The invention discloses a nanofiber for treating a bacterial infection diabetes wound surfaceand a preparation method and application thereof. The nano-fiber comprises a core curcumin nano-crystal and a shell polydopamine nano-tube. The used natural product curcumin has good biological safety, can induce M2 type polarization of macrophages, and further promotes wound healing. The polydopamine material has the advantages of excellent biocompatibility, good near-infrared conversion performance, remarkable photo-thermal antibacterial effect and the like. The nanofiber is simple in preparation process, low in cost, good in repeatability and suitable for large-scale production. The prepared nanofiber has good antibacterial and diabetes wound repair promoting capabilities, and has very high clinical application value.

Description

technical field [0001] The invention relates to a nanofiber for treating bacterial infection of diabetic wounds, a preparation method and application thereof, and belongs to the field of pharmaceutical preparations and the technical field of biomedicine. Background technique [0002] The wounds of diabetic patients are difficult to heal, and they have high morbidity and mortality worldwide. The restoration of skin integrity, homeostasis and protective function after injury is crucial to the quality of life and survival. The hindered healing of diabetic wounds is often accompanied by bacterial infection, which will not only aggravate wound deterioration, delay healing, and even lead to sepsis and eventually death. Currently, active ingredients such as antibiotics, anti-inflammatory drugs, exosomes, growth factors, etc. provide feasible options for fighting bacterial infections, inhibiting inflammatory responses, and accelerating wound healing. However, the above-mentioned th...

Claims

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Application Information

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IPC IPC(8): A61K9/00A61K41/00A61K31/12A61P17/02A61P3/10A61P31/04
CPCA61K9/0092A61K41/0052A61K31/12A61P17/02A61P3/10A61P31/04A61K2300/00
Inventor 陈刚徐洲
Owner YANGZHOU UNIV
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