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Preparation method of a three-dimensional composite porous scaffold and three-dimensional composite porous scaffold

A technology of three-dimensional composite and porous scaffolds, which is applied in the preparation of three-dimensional composite porous scaffolds, and in the field of three-dimensional composite porous scaffolds, can solve the problems of pore-forming agent residues and lack of interconnected pore structures in scaffolds, and achieve high porosity and easy promotion Good use and interoperability

Active Publication Date: 2022-06-28
SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, a variety of preparation methods of three-dimensional porous scaffolds, including multi-layer alternating electrospinning, mechanical extrusion, template method and adding porogens, etc., but the prepared scaffolds lack interconnected pore structures and may have disadvantages such as pore-forming agent residues.

Method used

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  • Preparation method of a three-dimensional composite porous scaffold and three-dimensional composite porous scaffold
  • Preparation method of a three-dimensional composite porous scaffold and three-dimensional composite porous scaffold
  • Preparation method of a three-dimensional composite porous scaffold and three-dimensional composite porous scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] S1: Take dichloromethane and N,N-dimethylformamide in a ratio of 4:1 by volume, and mix the dichloromethane and N,N-dimethylformamide evenly to obtain an organic solvent, and then according to The mass ratio of the organic solvent to the sum of the mass of polycaprolactone (PCL) and cellulose nanocrystals (CNC) is 16%. Weigh PCL and CNC, and stir and mix PCL and CNC with the organic solvent to dissolve them. And according to the electrospinning parameters of a voltage of 18kV, a sample injection speed of 0.8mL / h, a distance between the needle and the receiver of 20cm, and a needle of 20G, electrospinning was performed to obtain a nanofiber membrane with a diameter of 200-600nm; wherein , and the mass ratio of CNC added to PCL is 3:100;

[0042] S2: Mix according to the volume ratio of 1:1 to obtain a mixed solvent of absolute ethanol and deionized water, then weigh the nanofiber membrane obtained in step S1, weigh 4g of the nanofiber membrane and add it to 200mL of the ...

Embodiment 2

[0045] S1: Take dichloromethane and N,N-dimethylformamide in a ratio of 3:2 by volume, and mix the dichloromethane and N,N-dimethylformamide evenly to obtain an organic solvent, then follow The mass ratio of the organic solvent to the sum of the mass of polycaprolactone (PCL) and cellulose nanocrystals (CNC) is 20%. Weigh PCL and CNC, and stir and mix PCL and CNC with the organic solvent to dissolve them. And according to the electrospinning parameters of voltage 18kV, sample injection speed 0.8mL / h, the distance between the needle and the receiver is 20cm, and the needle is 20G, electrospinning is performed to obtain a nanofiber membrane with a diameter of 200-600nm;

[0046] S2: mix according to the volume ratio of 1:2 to obtain a mixed solvent of absolute ethanol and deionized water, then weigh the nanofiber membrane obtained in step S1, weigh 4g of the nanofiber membrane and add it to 200mL of the mixed solvent, and mechanically stir and break it into intermittent Accelera...

Embodiment 3

[0049] In step S1, the mass ratio of the organic solvent to the sum of the mass of PCL and CNC was 14%, and other preparation steps were basically the same as those in Example 1, and product C was obtained.

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Abstract

The invention discloses a preparation method of a three-dimensional composite porous scaffold and a three-dimensional composite porous scaffold, and relates to the technical field of biological scaffolds, including S1: dissolving polycaprolactone and cellulose nanocrystals in an organic solvent, mixing them uniformly and performing electrospinning , to obtain a nanofiber membrane; S2: mechanically stir and crush the nanofiber membrane in a mixed solvent, sieve to obtain short nanofiber filaments, and suction filter to obtain a wet material; S3: disperse the wet material in a mixture of absolute ethanol and deionized water in the mixed solution, and conduct thermal self-aggregation reaction at 50-57° C. for 5 minutes to obtain aggregates, and then freeze-dry in a vacuum freeze dryer to obtain a three-dimensional composite porous scaffold. The preparation method of a three-dimensional composite porous support and the three-dimensional composite porous support disclosed by the invention adopt a thermal self-aggregation method to prepare the composite porous support, and obtain a porous support with high porosity and good intercommunication.

Description

technical field [0001] The invention relates to the technical field of tissue engineering materials, in particular to a preparation method of a three-dimensional composite porous scaffold and the three-dimensional composite porous scaffold. Background technique [0002] In recent years, the rapid development of bone tissue engineering provides new technical possibilities for bone defect repair. Tissue engineering is the combination of cells, materials, and processing methods to prepare scaffolds that can serve as engineered three-dimensional microenvironments for cell adhesion, proliferation, differentiation, and extracellular matrix formation. Combined with appropriate biochemical and physicochemical factors, the microenvironment determined by the physical and chemical properties of the scaffold plays a crucial role in cell function and subsequent tissue regeneration, thereby improving or replacing the original biological tissue. [0003] In the extracellular matrix of nat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/56A61L27/50A61L27/18A61L27/20D04H1/728
CPCA61L27/56A61L27/50A61L27/18A61L27/20D04H1/728A61L2430/02A61L2400/12C08L67/04C08L1/02
Inventor 刘义王云鲁越
Owner SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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