Preparation method and application of rice bran polysaccharide zinc chelate
A technology of zinc chelate and rice bran polysaccharide, applied in the field of preparation of polysaccharide zinc chelate, can solve the problems of inability to scale up production, low yield, complicated process and the like, and achieve the effects of improving yield and improving purity
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Embodiment 1
[0040] (1) Pulverize the rice bran meal and pass through a 60-mesh sieve;
[0041] (2) Take 2kg of sieved rice bran meal and mix it with α-amylase and neutral protease, wherein the addition amount of α-amylase is 40U / g rice bran meal, and the addition amount of neutral protease is 3000U / g rice bran meal;
[0042] (3) Add water to the mixed material according to the solid-to-liquid mass ratio of 1:15 for batching, after stirring evenly, treat it in a hydraulic oscillation device for 8 minutes, and the temperature is room temperature;
[0043](4) Adjust the pH of the above-mentioned extraction mixture to 5.5 with citric acid, add phytase, xylanase and cellulase respectively, the enzymolysis temperature is 45°C, and the enzymolysis time is 1.5h, wherein the added amount of phytase is 120U / g rice bran meal, the addition amount of xylanase is 2000U / g rice bran meal, the addition amount of cellulase is 500U / g rice bran meal;
[0044] (5) Heat the enzymolysis solution to 100°C and ...
Embodiment 2
[0053] (1) Pulverize the rice bran meal and pass through an 80-mesh sieve;
[0054] (2) Weigh 2 kg of sieved rice bran meal, mix with α-amylase and neutral protease, wherein the addition amount of α-amylase is 80U / g rice bran meal, and the addition amount of neutral protease is 4000U / g rice bran meal;
[0055] (3) Add water to the mixed material according to the solid-to-liquid mass ratio of 1:20 for batching, after stirring evenly, treat it in a hydraulic oscillation device for 10 minutes, and the temperature is room temperature;
[0056] (4) Adjust the pH of the above-mentioned extraction mixture to 5.0 with citric acid, add phytase, xylanase and cellulase respectively, the enzymolysis temperature is 50°C, and the enzymolysis time is 2.0h, wherein the added amount of phytase is 150U / g rice bran meal, the addition amount of xylanase is 3000U / g rice bran meal, the addition amount of cellulase is 750U / g rice bran meal;
[0057] (5) Heat the enzymolysis solution to 95°C and ke...
Embodiment 3
[0066] (1) Pulverize the rice bran meal and pass through an 80-mesh sieve;
[0067] (2) Take 2kg of sieved rice bran meal, mix with α-amylase and neutral protease, wherein the addition amount of α-amylase is 120U / g rice bran meal, and the addition amount of neutral protease is 5000U / g rice bran meal;
[0068] (3) Add water to the mixed material according to the solid-to-liquid mass ratio of 1:25 for batching, after stirring evenly, treat it in a hydraulic oscillation device for 12 minutes, and the temperature is room temperature;
[0069] (4) Adjust the pH of the above-mentioned extraction mixture to 4.5 with citric acid, add phytase, xylanase and cellulase respectively, the enzymolysis temperature is 55°C, and the enzymolysis time is 2.5h, wherein the added amount of phytase is 180U / g rice bran meal, the addition amount of xylanase is 4000U / g rice bran meal, the addition amount of cellulase is 1000U / g rice bran meal;
[0070] (5) Heat the enzymolysis solution to 90°C and ke...
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