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Toona sinensis regeneration technology based on seed coat callus differentiation

A technology of callus and Chinese toon, applied in the field of Chinese toon regeneration technology based on callus differentiation of seed coat, can solve the problems of low reproduction coefficient, unfavorable rapid reproduction, slow speed, etc., achieve high reproduction coefficient, and facilitate the popularization and cultivation of fine varieties , fast effect

Active Publication Date: 2021-08-06
HUBEI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there is serious separation of traits between the offspring of seed propagation, and the offspring have low reproduction coefficient and slow speed through methods such as cutting seedlings, burying roots, and tillering.

Method used

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  • Toona sinensis regeneration technology based on seed coat callus differentiation
  • Toona sinensis regeneration technology based on seed coat callus differentiation
  • Toona sinensis regeneration technology based on seed coat callus differentiation

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Embodiment 1 One of method embodiment of the present invention

[0029] 1) Sterile seed acquisition

[0030] After removing the wings of Chinese toon seeds, wash them with clean water for 1 hour, soak them in sterile water for 12 hours, then soak them in 70% medical alcohol for 3 minutes, then rinse them with sterile water for 5 times, drain the water on the surface of the seeds on sterile filter paper, and set aside;

[0031] 2) Induction of seed coat callus

[0032] The seeds in the above steps are in the induction medium (basic medium MMS+0.01mg / LTDZ+0.3mg / L IAA+0.01mg / L 2,4-D+mass percentage of 3% sucrose+mass percentage 0.8% agar), In the dark, temperature 24°C, and air humidity 70%, culture for 1w, then move to 70% air humidity, light 13h / darkness 11h, light temperature 26°C / dark temperature 24°C, light intensity 10-20μmol photons / m 2 Under the culture condition of / s, subculture once every 2w. After 2w, the seed coat callus begins to form, and the callus prese...

Embodiment 2

[0043] Embodiment 2 The second embodiment of the method of the present invention

[0044] The operation steps of this embodiment are the same as those of Embodiment 1, the only difference being that each parameter is different, as follows:

[0045] 1) Sterile seed acquisition

[0046] After removing the wings of Chinese toon seeds, wash them with clean water for 2 hours, soak them in sterile water for 12 hours, then soak them with 70% medical alcohol for 5 minutes, then rinse them with sterile water for 3 times, drain the water on the surface of the seeds on sterile filter paper, and set aside;

[0047] 2) Induction of seed coat callus

[0048] The seeds in the above steps were cultured for 1w in the induction medium (same as in Example 1) in the dark, at a temperature of 24°C and at an air humidity of 70%, and then moved to an induction medium with an air humidity of 70%, light for 13h / darkness for 11h, and light temperature. 26℃ / dark temperature 24℃, light intensity 10-20μ...

Embodiment 3

[0059] Embodiment 3 The third embodiment of the method of the present invention

[0060] This embodiment is the same as the operation steps of embodiment 1 or 2, the difference is only that each parameter is different, specifically as follows:

[0061] 1) Sterile seed acquisition

[0062] After removing the wings of Chinese toon seeds, wash them with clean water for 1.5 hours, soak them in sterile water for 12 hours, then soak them with 70% medical alcohol for 4 minutes, and then rinse them with sterile water for 3 times. ;

[0063] 2) Induction of seed coat callus

[0064] The seeds in the above steps were cultured for 1w in the induction medium (same as in Example 1) in the dark, at a temperature of 24°C and at an air humidity of 70%, and then moved to an induction medium with an air humidity of 70%, light for 13h / darkness for 11h, and light temperature. 26℃ / dark temperature 24℃, light intensity 10-20μmol photons / m 2 Under the culture condition of / s, subculture once eve...

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Abstract

The invention discloses a toona sinensis regeneration technology based on seed coat callus differentiation. The technology characterized by comprising the steps of sterile seed obtaining, seed coat callus induction, seed coat callus growth, seed coat callus differentiation, seedling rooting induction culture, seedling hardening, transplanting and the like. By adopting creative process steps, technical parameters and other factors, the rooting seedling survival rate of 97.5% or above can be obtained. The method at least has the following beneficial effects: (1) the propagation coefficient is high and the propagation speed is high; (2) large-scale production of improved varieties can be achieved, and the requirement for plastic greenhouse cultivation or greenhouse cultivation is met; (3) all hereditary characteristics of the original high-quality strain can be maintained, the germplasm consistency of the variety is realized, and the popularization and cultivation of the high-quality variety are facilitated; and (4) technical support is provided for functional gene research and transgenic molecular breeding of toona sinensis. The technology has a good application value.

Description

【Technical field】 [0001] The invention relates to plant regeneration technology, in particular to a Chinese toon regeneration technology based on seed coat callus differentiation. 【Background technique】 [0002] Chinese toon (Toona sinensis (A.Juss) Roem), Meliaceae (Meliaceae) Toona genus (Toona Roem), a large perennial deciduous tree, a unique tree species in my country, is a traditional woody vegetable in China. Its tender buds are pleasant and nutritious , both for medical and dietary purposes, ranking first among the famous and high-quality vegetables, and cherished by thousands of households. The sprouts secrete insect-repelling substances during the growth process, without pesticide pollution, and are veritable green food. Toona sinensis sprouts are rich in chemical components and nutrients, and their edible, nutritional and medicinal values ​​are extremely high. Source" woody vegetables, known as "Chinese green health vegetables" abroad. [0003] Because Chinese too...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 王友如艾薇李玲黄蓉邱正明刘军
Owner HUBEI NORMAL UNIV