Method for improving gel property of myofibrillar protein by controlling interaction of protein-EGCG
A myofibrillar protein and gel technology, which is applied in the direction of food ingredients as thickeners, food ingredients as humectants, preservation of meat/fish with chemicals, etc. , slow glycosylation reaction rate and other problems, to achieve the effect of easy control of reaction degree, avoid deterioration of gel properties, and short reaction period
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment Construction
[0017] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.
[0018] 1. Dissolve amylopectin and EGCG in 20 mM PBS (containing 0.6 M NaCl, pH 7.4) and mix them at a ratio of 1:1~8:1. Shake the mixed solution for 12 h in the dark to form amylopectin-EGCG complexes.
[0019] 2. Add the pullulan / EGCG mixture to the myofibrillar protein, mix well after treatment with the hydroxyl radical oxidation system, and oxidize at 4°C for 12 h. The concentration of myofibrillar protein in the final reaction system was 40 mg / mL, the concentration of EGCG was 80 μM / g protein, and the concentration of hydroxyl radical oxidation system was 10 mM H 2 o 2 , 20 μM FeCl 3 , 100 μM ascorbic acid.
[0020] 3. Preparation of myofibrillar protein gel: Weigh 5 g of the reacted sample, put it in a 10 mL beaker, put it in a water bath, heat it from 25°C to 72°C, keep it warm for 10 minutes, take it out, and freeze the sample imme...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com