Preparation method of excrement inoculum substitute in preparation process of pulvis glycyrrhizae praeparatus

Abstract

The invention belongs to the field of traditional Chinese medicine processing, and discloses a preparation method of an excrement inoculum substitute in a preparation process of rulvis glycyrrhizae praeparata. The preparation method comprises the following steps of (1) preparing an excrement inoculation liquid, namely, adding 0.1 M of sterilized and anaerobic treated PBS (Phosphate Buffer Solution) with the pH value of 7.0 into excrement, carrying out uniform vortex mixing, passing through a filtering membrane, and taking filtrate to obtain the excrement inoculation liquid; (2) determining the enterotypes of the intestinal microecology of the excrement inoculation liquid; (3) inoculation, namely, adding the excrement inoculation liquid into a fermentation culture medium in a fermentation tank, performing anaerobic fermentation, and performing in-vitro culture of enteric microorganisms to obtain fermentation liquid containing the enteric microorganisms; (4) continuously fermenting for 10-12 days, and collecting fermentation liquid when florae in the fermentation tank are stable; and (5) wrapping licorice powder with a semipermeable membrane (with the aperture of 2-5 microns), immersing the licorice powder in the bacterial liquid, fermenting at 37 DEG C for 3-14 days, taking out and drying. According to the method, the excrement is selected to be subjected to appropriate enlarged culture according to different enterotypes, the excrement inoculum which is consistent and controllable in quality is produced, and a feasible and operable production scheme is provided for standardized production of the pulvis glycyrrhizae praeparatus.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine processing, in particular to a method for preparing a feces inoculum substitute in the preparation process of Rhizoma chinensis. Background technique [0002] Renzhonghuang, the name of traditional Chinese medicine, has the effect of clearing away heat and cooling blood, purging fire and detoxifying. It is often used for Tianxing fever, febrile disease spots, severe fever and polydipsia, acne and blood heat, erysipelas, and sores. Renzhonghuang is also known as "licorice yellow", "Ganzhonghuang" and so on. Its processing method was first seen in Yuan Zhu Zhenheng's "Danxi Xinfa", which said: "Renzhonghuang, put the licorice powder into the bamboo tube, and the bamboo cork. Both ends are soaked in dung tanks in the winter moon, taken out in the beginning of spring and dried in the shade, broken bamboo to get grass, and dried in the sun." [0003] However, the temperature of the cesspit ...

AI Technical Summary

Problems solved by technology

[0003] However, the temperature of the cesspit is uncontrollable, and individuals are affected by regions, diets, ages, and lifestyles, and the fecal flora differs greatly, resulting in large differences in the flora structure and function of the inoculum during the production of Renzhonghuang. "Danxi The traditional process recorded in "Heart Method" does not conform to ethics, and the quality and effect of human quinone produced are unstable, which does not meet the requirements of biosafety