Preparation method of excrement inoculum substitute in preparation process of pulvis glycyrrhizae praeparatus
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A preparation process and technology of inoculum, which is applied in the field of preparation of fecal inoculum substitutes, can solve problems such as excessive differences in flora structure and function, unstable effect, and unethical traditional processes, and achieve great economic significance and promote The effect of development, efficiency improvement and stability
Inactive Publication Date: 2021-08-06
湖南海路生物科技有限公司 +2
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[0003] However, the temperature of the cesspit is uncontrollable, and individuals are affected by regions, diets, ages, and lifestyles, and the fecal flora differs greatly, resulting in large differences in the flora structure and function of the in
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Embodiment 1
[0024] A method for preparing a substitute for feces inoculum in the preparation process of Rhizoma chinensis, comprising the following steps:
[0025] (1) Making feces inoculum: take feces, add sterilized and anaerobically treated pH7.0, 0.1MPBS, vortex mix, pass through an 800-mesh filter membrane, and take the filtrate as the feces inoculum;
[0026] (2) Determine the enterotypes of the intestinal microecology of the fecal inoculum: use fluorescent real-time quantitative PCR (qPCR) to quantitatively analyze the content of Bacteroides, Prevotella and total bacteria, and calculate the Bacteroides according to the test results The proportion of the genus and Prevotella in the total bacterial population determined that the faeces belonged to the Bacteroides enterotype;
[0027] (3) Inoculation: Add the feces inoculum solution into the fermentation medium in the fermenter, perform anaerobic fermentation at 37°C, and carry out in vitro culture of intestinal microorganisms to obta...
Embodiment 2
[0035] The preparation method of the feces inoculum substitute in the human zhonghuang preparation process comprises the following steps:
[0036] (1) Making feces inoculum: take feces, add sterilized and anaerobically treated pH7.0, 0.1MPBS, vortex mix, pass through an 800-mesh filter membrane, and take the filtrate as the feces inoculum;
[0037] (2) Determine the enterotypes of the intestinal microecology of the fecal inoculum: use fluorescent real-time quantitative PCR (qPCR) to quantitatively analyze the content of Bacteroides, Prevotella and total bacteria, and calculate the Bacteroides according to the test results The proportion of the genus and Prevotella in the total bacterial population determined that the stool belonged to the Prevotella enterotype;
[0038] (3) Inoculation: Add the feces inoculum to the fermentation medium in the fermenter, perform anaerobic fermentation at 37°C, and carry out in vitro culture of intestinal microorganisms to obtain a fermentation ...
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Abstract
The invention belongs to the field of traditional Chinese medicine processing, and discloses a preparation method of an excrement inoculum substitute in a preparation process of rulvis glycyrrhizae praeparata. The preparation method comprises the following steps of (1) preparing an excrement inoculation liquid, namely, adding 0.1 M of sterilized and anaerobic treated PBS (Phosphate Buffer Solution) with the pH value of 7.0 into excrement, carrying out uniform vortex mixing, passing through a filtering membrane, and taking filtrate to obtain the excrement inoculation liquid; (2) determining the enterotypes of the intestinal microecology of the excrement inoculation liquid; (3) inoculation, namely, adding the excrement inoculation liquid into a fermentation culture medium in a fermentation tank, performing anaerobic fermentation, and performing in-vitro culture of enteric microorganisms to obtain fermentation liquid containing the enteric microorganisms; (4) continuously fermenting for 10-12 days, and collecting fermentation liquid when florae in the fermentation tank are stable; and (5) wrapping licorice powder with a semipermeable membrane (with the aperture of 2-5 microns), immersing the licorice powder in the bacterial liquid, fermenting at 37 DEG C for 3-14 days, taking out and drying. According to the method, the excrement is selected to be subjected to appropriate enlarged culture according to different enterotypes, the excrement inoculum which is consistent and controllable in quality is produced, and a feasible and operable production scheme is provided for standardized production of the pulvis glycyrrhizae praeparatus.
Description
technical field [0001] The invention relates to the field of traditional Chinese medicine processing, in particular to a method for preparing a feces inoculum substitute in the preparation process of Rhizoma chinensis. Background technique [0002] Renzhonghuang, the name of traditional Chinese medicine, has the effect of clearing away heat and cooling blood, purging fire and detoxifying. It is often used for Tianxing fever, febrile disease spots, severe fever and polydipsia, acne and blood heat, erysipelas, and sores. Renzhonghuang is also known as "licorice yellow", "Ganzhonghuang" and so on. Its processing method was first seen in Yuan Zhu Zhenheng's "Danxi Xinfa", which said: "Renzhonghuang, put the licorice powder into the bamboo tube, and the bamboo cork. Both ends are soaked in dung tanks in the winter moon, taken out in the beginning of spring and dried in the shade, broken bamboo to get grass, and dried in the sun." [0003] However, the temperature of the cesspit ...
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