Glutathione synthetase culture process

Abstract

The invention discloses a glutathione synthetase culture process. The glutathione synthetase culture process comprises the following steps: A, a fermentation section: preparing materials, culturing seeds, performing pretreating, performing fermenting and performing centrifuging; and B, a curing section: performing pulping, performing homogenizing and breaking walls, performing centrifuging, performing heat treatment, performing nanofiltration, performing loading, performing mixing and dewatering, and performing airing at low temperature, and airing mixed and dewatered loaded polyester foam sheets in an airing chamber at low temperature of 20 DEG C to obtain the finished product of a glutathione synthetase preparation. Through the mode, the glutathione synthetase culture process particularly limits raw materials, performs high-temperature sterilization on a test tube, a shake flask, a fermentation tank and a culture medium, and particularly limits the sterilization temperature and time of the fermentation tank and the culture medium, so that the problem of infectious microbe infection is reduced, and increasing the yield is facilitated; and airing at low temperature is adopted, so that the production difficulty and cost are reduced.

Description

technical field [0001] The invention relates to the technical field of biological enzymes, in particular to a glutathione synthetase cultivation process. Background technique [0002] Glutathione is a tripeptide containing γ-amide bonds and sulfhydryl groups, which is composed of glutamic acid, cysteine ​​and glycine. Because glutathione can participate in the transformation of organisms, it can convert harmful poisons in the human body into harmless substances and excrete them from the body. It has anti-oxidation, integrated detoxification functions, and can also help the human body maintain normal immune system functions. , therefore, it is widely used in functional medicine and food fields such as anti-aging and immunity enhancement. [0003] The production method of glutathione includes enzymatic synthesis method, which uses glutathione synthetase to catalyze the synthesis of glutamic acid, cysteine ​​and glycine. Therefore, the quality of glutathione synthase is very i...

AI Technical Summary

Problems solved by technology

However, glutathione synthase has many production processes and is easily infected by miscellaneous bacteria, which affects the quality of glutathione synthase, and the yield is low, so it needs to be improved.