Bacterial strain with heterotrophic nitrification and aerobic denitrification function and application thereof

An aerobic denitrification and heterotrophic nitrification technology, which is applied in the field of environmental microorganisms, can solve the problems of water quality deterioration of water ecological balance, aquatic organisms poisoning, etc., and achieves obvious aerobic denitrification ability and good ammonia nitrogen removal effect.

Inactive Publication Date: 2021-08-10
FISHERIES RES INST ANHUI ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the nitrogen content in the water body exceeds the environmental capacity of the receiving water body, the ecological balance

Method used

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  • Bacterial strain with heterotrophic nitrification and aerobic denitrification function and application thereof
  • Bacterial strain with heterotrophic nitrification and aerobic denitrification function and application thereof
  • Bacterial strain with heterotrophic nitrification and aerobic denitrification function and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0032] Example 1:

[0033] A strain having a heterotrophic nitrified oxygen-denddimized function, the strain is a pf1 of the Achromobacter Xylosoxidans, and the collection location is located in Anhui Lantian Special Town, Anhui Lantian, Wuhu City, Anhui Province. Its 2021 On March 25, he was deposited in China's Typical Casting Safety Center. The preservation of No. CCTCC NO: M2021273; Sales address is in Wuhan University, No. 299, Wuchang District, Wuhan, Hubei Province.

[0034] 1, separation and purification of strains

[0035] The water sample collected from the turtle breeding pond is enriched in a proportion of a volume ratio of 10% to the enriched, and the culture is cultured from 30 ° C, 150R / min shaker; 10% per 48 hours The volume ratio is replaced with fresh heterotrophic nitrification medium, continuous culture 20D.

[0036] Among them, the formulation of heterotrophic noduled enriched medium is: (NH 4 ) 2 SO 4 0.472 g, sodium succinate 4.052 g, 50 ml vitron salt sol...

Example Embodiment

[0046] Example 2, Culture and Nitrogen Performance Analysis of Nonorobacterium PF1

[0047] Picking the colonies of the complement of the complex mesenchyma separated from Example 1 to the R2A liquid medium, 30 ° C gas bath shaker 200 rpm culture for 18 h, centrifuge at 4000 R / min, collected by the bacteria, and resuspended with isometric The bacterium was inoculated with a 1% (volume ratio) inoculum, and DMA (nitrate nitrogen source) and DMB (nitrite as nitrogen source) were in liquid medium. At 30 ° C, 200 rpm shake flask culture, timing sample measurement cell light density (OD600), total nitrogen (TN), ammonia nitrogen (NH 4 + -N), nitrate (NO 3 - -N) and nitrite (NO 2 - The concentration of -N), according to the Growth curve of the OD600 value, by analyzing the nitrogen-nitrogen resistance of the strain by analyzing the removal rate of total nitrogen.

[0048] The R2A liquid medium formulation is: 0.5 g / l, yeast extract 0.5 g / L, starch 0.5 g / l, 0.5 g / l, glucose 0...

Example Embodiment

[0054] Example 3: Nonorobacterium pf1 actual denitrification application

[0055] Each of the 1000ml initial COD is 760mg / L of turtle plant breeding wastewater to add 3L beaker, add 15g of sodium citrate, add carbon supply, turtle factory cultured wastewater COD increase to 8160mg / L; add 0.5g The R2A medium in Example 2 enlarged the cultured PF1 wet bacteria, placed under room temperature (18 to 25 ° C), and the dissolved oxygen was maintained with a aeration device in 2 ppm or more. At the same time, the same treatment was carried out as a control group after breeding wastewater at the same time without the turtle of the PF1. After 24 hours, the sample was removed, centrifuged, and the total nitrogen, total phosphorus and COD content in the supernatant were detected. Specifically, as shown in the following table:

[0056] Table 1 Effect of Niogobacillus PF1 removes nitrogen phosphorus in wastewater

[0057]

[0058] Table 1 shows that the plant turtle breeding wastewater co...

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Abstract

The invention provides a bacterial strain with heterotrophic nitrification and aerobic denitrification functions and application. The bacterial strain is (Achromobacter xylosoxidans) pf1, the bacterial strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M2021273. The bacterial strain can synchronously perform nitrification and denitrification for nitrogen removal by taking organic carbon as a carbon source under an aerobic condition, and is suitable for removal of organic wastewater containing ammonia nitrogen, nitrate and nitrite. In the ammonia nitrogen wastewater with the initial concentration of 175 mg/L, the total nitrogen removal rate can reach 100%, the accumulation of intermediate products is less, and secondary pollution is avoided; in nitrate or nitrite wastewater with the initial concentration of 100 mg/L, the total nitrogen removal rate can reach 87.1% or 65.1%. After the industrial turtle breeding wastewater is treated, nitrogen and phosphorus in the industrial turtle breeding wastewater can be remarkably removed.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to an Achromobacter strain with heterotrophic nitrification-aerobic denitrification functions and its application in the treatment of ammonia nitrogen, nitrate nitrogen and nitrite nitrogen wastewater. Background technique [0002] At present, a major problem facing my country's water environment protection is that the concentration of ammonia nitrogen in the water far exceeds the self-purification capacity of the sewage. The national surface water environmental quality in the first half of 2016 shows that 11.3% of my country’s top ten river basins are inferior to Category V, of which COD, ammonia nitrogen and total phosphorus are the main pollution indicators, and the oxygen-consuming pollutant ammonia nitrogen has become a major factor affecting the environmental quality of my country’s surface water bodies. primary indicator. The severe situation of ammonia...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F3/30C02F101/16C02F101/38C02F101/30C12R1/025
CPCC02F3/34C02F3/302C02F3/308C02F2101/16C02F2101/163C02F2101/166C02F2101/38C02F2101/30
Inventor 高娜梁阳阳杨坤李静方婷赵秀侠卢文轩李书杨
Owner FISHERIES RES INST ANHUI ACAD OF AGRI SCI
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