Pseudomonas nitroreducens. strain and application thereof

A technology based on pseudomonas and nitro, which is applied in the direction of bacteria, microbial-based methods, water/sludge/sewage treatment, etc., can solve problems such as low efficiency, difficult balance and coordination, and complex denitrification process, and achieve reduction The effect of operation and maintenance cost, simplification of treatment process and improvement of treatment efficiency

Inactive Publication Date: 2021-08-13
FISHERIES RES INST ANHUI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This makes it difficult to balance and coordinate the traditional nitrification and denitrification process, resulting in a very complicated and inefficient denitrification process

Method used

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  • Pseudomonas nitroreducens. strain and application thereof
  • Pseudomonas nitroreducens. strain and application thereof
  • Pseudomonas nitroreducens. strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A strain of Pseudomonas nitroreducens. The strain is Pseudomonas nitroreducens. pf2, which was preserved in the China Center for Type Culture Collection on March 25, 2021, and the preservation number is: CCTCC NO : M2021274; Address: Wuhan University Campus, No. 299 Bayi Road, Wuchang District, Wuhan City, Hubei Province.

[0033] 1. Enrichment and isolation of strains

[0034] 1.1 Take the bottom mud collected from part of the culture pond and mix it with physiological saline and let it stand still, and take the supernatant as the sample dilution stock solution. The diluted stock solution was added to the heterotrophic nitrification enrichment medium at a volume ratio of 10%, and cultured on a shaker at 30° C. and 200 rpm / min for 3 days.

[0035] Among them, heterotrophic nitrification enrichment medium: will (NH 4 ) 2 SO 4 0.472g, 4.052g sodium succinate, 50ml Vickers salt solution, add water to dissolve, and add distilled water to 1L, adjust the pH to 7.0. Wher...

Embodiment 2

[0045] Example 2, the removal ability of Pseudomonas nitroreductor pf2 to ammonia nitrogen and total nitrogen in wastewater.

[0046] Take NH 4 + -N is nitrogen source, sodium citrate is carbon source, NH in wastewater 4 + Under the condition of -N concentration of 175mg / L, the growth of strain pf2 and the removal ability of ammonia nitrogen and total nitrogen were investigated.

[0047] Pick the colonies of the rescreened bacteria isolated in Example 1 to heterotrophic nitrifying ammonium nitrogen medium (NM) 30°C air bath shaker at 220rpm and cultivate for 18h, specifically: take the liquid strain according to the volume ratio of the medium (2ml) was inoculated into 200ml NM (heterotrophic nitroammonium nitrogen medium). The formula of NM medium is: (NH 4 ) 2 SO 4 0.945g / L, sodium citrate 16.34g / L, MgSO 4 ·7H 2 O 1g / L, KH 2 PO 4 0.25g / L, Na 2 HPO 4 0.3g / L.

[0048] Depend on figure 2 It can be seen that the strain enters the logarithmic growth phase after ...

Embodiment 3

[0049] Embodiment 3, the removal ability of nitrate nitrogen in wastewater by Pseudomonas nitroreductor pf2

[0050] The strain pf2 uses nitrate as nitrogen source, and the aerobic denitrification ability of the strain is investigated by detecting the degradation of nitrogen source in wastewater. The initial TN concentration of nitrate wastewater was 147mg / L, and the initial NO 3 - -N concentration is 99mg / L, according to medium volume ratio is 1%, the liquid strain (2ml) of the rescreening bacterium isolated in Example 1 is inoculated in 200ml nitrate medium, at 30 ℃, rotating speed 220rpm cultivated under conditions. The formula of the culture medium is: KNO 3 0.722 g / L, sodium citrate 5g / L, MgSO 4 ·7H 2 O 1g / L, KH 2 PO 4 0.25g / L, Na 2 HPO 4 0.3g / L. Depend on figure 2 It can be seen that the OD600 of the strain pf2 reached 1.338 after being cultured for 16 hours. Such as Figure 4 As shown, at this time, the removal rate of the strain to nitrate nitrogen re...

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Abstract

The invention provides a Pseudomonas nitroreducens. strain and application thereof. The strain is Pseudomonas nitroreducens. Pf2, and is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO: M2021274. The strain can synchronously perform nitrification and denitrification by taking organic carbon as a carbon source under an aerobic condition, and is suitable for removing organic wastewater containing ammonia nitrogen or nitrate, the NH4 < + > -N removal rate can reach 92.7 percent, and the NO3 < - > -N removal rate can reach 100 percent; and the treatment process can be greatly simplified, the treatment efficiency is improved, and the operation and maintenance cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a nitroreductor pseudomonas strain with heterotrophic nitrification and aerobic denitrification function and application thereof. Background technique [0002] Water nitrogen pollution has become the most serious environmental problem in the world, and biological denitrification is the most economical and effective disposal method in water pollution treatment. Traditional biological nitrogen removal mainly includes nitrification and denitrification. Biological nitrification and denitrification processes are interdependent, but because there are huge differences in the physiology and biochemistry of nitrifying and denitrifying microorganisms, so the dissolved oxygen (Dissolved oxygen, DO), temperature, pH, growth rate, chemical oxygen demand (Chemical oxygen demand) ,COD) and nitrate and nitrite have different tolerance and performance. This makes it...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/30C02F101/16C12R1/38
CPCC12N1/20C02F3/34C02F3/303C02F3/305C02F2101/16C02F2101/163
Inventor 高娜梁阳阳杨坤李静方婷赵秀侠卢文轩洪大保
Owner FISHERIES RES INST ANHUI ACAD OF AGRI SCI
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