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Aeromonas salmonicida derived from turbot and application of Aeromonas salmonicida

A technology for killing Aeromonas salmonids and turbot, applied to medical preparations containing active ingredients, bacteria, antibacterial drugs, etc., can solve the problems of different protection ranges of bacteria strains, to ensure the quality and safety of aquatic products, reduce Effects of Antibiotic Misuse

Active Publication Date: 2021-08-10
烟台市海洋经济研究院(烟台市渔业技术推广站、烟台市海洋捕捞增殖管理站)
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AI Technical Summary

Problems solved by technology

However, in vaccine preparation, strains from different isolation sources have different protection ranges

Method used

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Embodiment Construction

[0010] The present invention will be specifically introduced below in conjunction with specific embodiments.

[0011] 1. Strain isolation, identification and virulence gene detection

[0012] 1. Strain isolation

[0013] The sample of the diseased turbot was collected from a turbot farm in Yantai, Shandong Province, with an individual mass of about 350 g. The diseased turbot manifested as: loss of appetite or even no food, no exercise, black body color, red mouth, red fin rays, and red, swollen and protruding anus. Autopsy found: liver congestion, intestinal swelling and congestion or fluid accumulation, kidney enlargement with milky white pus-like fluid.

[0014] Take dying individuals of sick turbot with typical symptoms, aseptically dissect internal organs, take liver, kidney and spleen, rinse with sterile water, grind, streak on nutrient agar plate added with 2wt% NaCl, incubate upside down at 28°C for 48h, Pick dominant colonies with consistent morphology for purificat...

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Abstract

The invention discloses a turbot-sourced Aeromonas salmonicida BHAS-1 and application thereof. The Aeromonas salmonicida BHAS-1 is Aeromonas salmonicida subspecies, is separated from internal organs of turbot with red mouth, fin ray hyperemia and red, swollen and convex anus symptoms, carries 14 virulence genes, and is obviously different from reported Aeromonas salmonicida with different sources; The Aeromonas salmonicida BHAS-1 is named as Aeromonas salmonicida BHAS-1, is preserved in China Center for Type Culture Collection on March 24, 2021, the preservation address is Wuhan, China, the preservation number is CCTCC NO: M 2021264, and the Aeromonas salmonicida BHAS-1 is classified and named as Aeromonas salmonicida BHAS-1. When the Aeromonas salmonicida BHAS-1 is used for preparing vaccines, turbot aeromonas diseases can be effectively prevented and controlled, the abuse of antibiotic drugs is reduced, and the quality safety of aquatic products is guaranteed.

Description

technical field [0001] The invention relates to a strain of bacteria and its application, in particular to a strain of Aeromonas salmonicida derived from turbot and its application, and belongs to the technical field of pathogenic microorganism screening. Background technique [0002] Turbot belongs to the genus Turbot of the Flounder family. Since it was introduced into my country in 1990 and broke through the artificial breeding technology, its breeding scale has continued to grow, successfully promoting the formation of the fourth wave of marine aquaculture industrialization in my country. It is the northern factory An important species for chemical farming. However, with the development of high-density intensive farming methods, serious bacterial diseases have appeared in the breeding process, which not only caused economic losses to the farming units, but also seriously restricted the green and healthy development of the aquaculture industry. Generally speaking, the use...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K39/02A61P31/04C12R1/01
CPCA61K39/02A61K2039/521A61K2039/552A61P31/04
Inventor 王鹤黄华李战军张建柏王力勇贺加贝刘蓬高雁
Owner 烟台市海洋经济研究院(烟台市渔业技术推广站、烟台市海洋捕捞增殖管理站)
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