Pseudomonas proteinans antibacterial aptamer and screening method thereof

Abstract

The invention relates to a nucleic acid aptamer, in particular to a pseudomonas proteinans antibacterial aptamer and a screening method thereof. The sequence of the aptamer is shown as SEQ.NO.1-5. The SELEX technologies such as reverse screening and distributed centrifugation are adopted, the nucleic acid aptamer capable of specifically recognizing and inhibiting the growth of the pseudomonas mumulosa is screened, and the influence of the nucleic acid aptamer on the pseudomonas mumulosa is represented by measuring the bacteriostasis rate. The high-frequency sequences can be objectively and effectively classified, an objective and effective basis is provided for precise selection and verification of subsequent nucleic acid aptamers, the method is simple and convenient to operate, the blindness of verification is greatly reduced, and the screening efficiency is improved. Five aptamers Y1, Y2, Y3, Y4 and Y10 with the highest relative important indexes are selected from screening results, and the antibacterial rates of the aptamers are 33.78%, 70.95%, 55.15%, 39.19% and 58.46% respectively. Therefore, the feasibility of screening the aptamer through the relative importance index is verified.

Description

technical field [0001] The invention relates to a nucleic acid aptamer, in particular to a Pseudomonas mutans antibacterial aptamer and a screening method thereof. Background technique [0002] Pseudomonas mutans belongs to the Pseudomonas family, Pseudomonas genus, Pseudomonas putida group, Ayucidal Pseudomonas species, is a straight or slightly curved, non-helical gram Bacteria negative bacilli, moving with unipolar hairs or several pole hairs, aerobic and non-spore forming, can cause diseases of sweetfish, large yellow croaker, rainbow trout, and grouper during aquaculture. In the cultivation of large yellow croaker, it is the most common. The main symptoms of large yellow croaker in the early stage of disease are decreased vitality, slow swimming, loss of appetite or complete loss, but there are no obvious symptoms on the body surface, and ulcers are occasionally seen; internal organs can be seen after necropsy Severe organ lesions, white nodules appear in the spleen, k...

AI Technical Summary

Problems solved by technology

Due to the widespread use of antibiotics, the resistance of pathogenic bacteria and the limitations of antibiotics used in aquaculture, it is gradually unavailable and ineffective in aquaculture during disease outbreaks, which poses a huge challenge to aquaculture. Therefore, it is necessary to seek alternatives to antibiotics to treat deformation Pseudomonas disease-causing health drug becomes research focus

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