Method for improving quality of mushroom bran feed through multi-strain mixed fermentation
A mixed fermentation, multi-strain technology, applied in microorganism-based methods, animal feed, animal feed, etc., can solve the problems of high content of anti-nutritional factors, high content of crude fiber, difficult to use, etc., to inhibit proliferation and improve absorption. Effectiveness of utilization and maintenance of bacterial flora balance
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Embodiment 1
[0026] This embodiment provides a method for improving the quality of bacterium chaff feed by multi-strain mixed fermentation, comprising the following steps:
[0027] Step 1, microbial strain expansion:
[0028] Lactobacillus acidophilus is inoculated into the liquid medium for cultivation, the inoculation amount is 5-15%, and the cultivation temperature is 38-40°C. Among them, the culture medium used for strain culture is MRS medium, and the preparation method is: weigh 20.0g of glucose, 15.0g of peptone, 15.0g of beef extract, 7.5g of yeast extract, 7.5g of sodium acetate, 2.0g of ammonium citrate, hydrogen phosphate Dipotassium 2.0g, magnesium sulfate 0.3g, add water to dissolve to 1000ml, adjust pH=6.8, sterilize at 121°C for 20-30min;
[0029] The Bacillus subtilis is inoculated into the liquid medium for cultivation, the inoculation amount is 5-15%, and the cultivation temperature is 38-40°C. Among them, the culture medium used for strain cultivation is LB medium, and...
Embodiment 2
[0037] The present embodiment adopts single factor experiment to determine the optimal fermentation condition in embodiment 1, and concrete method and result are as follows:
[0038] 1. Method
[0039] The fermentation experiment was carried out under the condition of natural pH with the fungus chaff as the fermentation substrate, and the fermentation temperature was 40°C. After the fermentation is over, it is dried and pulverized at about 75°C and passed through a 40-mesh sieve to measure indicators such as total acid, small peptides, and total viable bacteria before and after fermentation.
[0040] (1) Inoculation amount
[0041] The inoculation amount is 5%, 10%, and 15% as a single factor, and each factor is repeated three times. The ratio of the three strains is 1:1:1, the ratio of material to water is 1:1, the fermentation temperature is 40°C, and the fermentation time is 84h .
[0042] (2) Ratio of material to water
[0043] Set up 3 treatment groups, add distilled ...
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