Method for improving quality of mushroom bran feed through multi-strain mixed fermentation

A mixed fermentation, multi-strain technology, applied in microorganism-based methods, animal feed, animal feed, etc., can solve the problems of high content of anti-nutritional factors, high content of crude fiber, difficult to use, etc., to inhibit proliferation and improve absorption. Effectiveness of utilization and maintenance of bacterial flora balance

Pending Publication Date: 2021-08-13
上海源耀农牧科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the current problems such as high crude fiber content and low digestibility of fungus bran feed; high water content, easy to mildew; high content of anti-nutritional factors, difficult to use, etc., the present invention

Method used

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  • Method for improving quality of mushroom bran feed through multi-strain mixed fermentation
  • Method for improving quality of mushroom bran feed through multi-strain mixed fermentation
  • Method for improving quality of mushroom bran feed through multi-strain mixed fermentation

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0025] Example 1

[0026] This embodiment provides a method of mixing a multi-strain mixed fermentation to improve the quality of the bacteria feed, including the following steps:

[0027] Step 1, microbial strains Primulation:

[0028] Eosinic acid bacteria were inoculated into liquid medium for culture, and the amount of inoculation was 5-15%, and the culture temperature was 38-40 ° C. Among them, the medium used in strain culture is MRS medium, and the formulation method is: weighing glucose 20.0g, protein 胨 15.0g, beef paste 15.5 g, yeast paste 7.5g 7.5g, citrate 2.0g, hydrogen phosphate 2.0 g of diopassium, 0.3 g of magnesium sulfate, dissolved to 1000 ml, regulate pH = 6.8, 121 ° C for 20-30 min;

[0029] Bacillus were seeded into a liquid medium for culture, and the inoculation amount was 5-15%, and the culture temperature was 38-40 ° C. Among them, the medium used in strain culture is LB medium, and the formulation method is: weigh NaCl10.0g, protein 胨 10.0g, yeast paste 5...

Example Embodiment

[0036] Example 2

[0037] This embodiment uses a single factor experiment to determine the optimal fermentation conditions in Example 1, the specific methods and results as follows:

[0038] Method

[0039] The fermentation experiment was carried out under the conditions of fermentation substrate, natural pH, and the fermentation temperature was 40 ° C. After the fermentation, drying pulverized over 40 mesh sieve at about 75 ° C, measuring indicators such as total acids, small peptides, total live bacteria before and after fermentation.

[0040] (1) Volume

[0041] The amount of inoculation is 5%, 10%, 15% is a single factor. Each factor is repeated. The ratio of the three strains is 1: 1: 1, the water ratio is 1: 1, the fermentation temperature is 40 ° C, fermentation time 84h .

[0042] (2) Water ratio

[0043] The three processing groups are set, and the amount of distilled water is added in accordance with 1: 1, 1: 1.5, 1: 2, respectively, and the inoculation amount is selecte...

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Abstract

The invention provides a method for improving the quality of a mushroom bran feed through multi-strain mixed fermentation, which comprises the following steps: by taking mushroom bran after three times of fruiting as a fermentation substrate and taking lactobacillus acidophilus, saccharomyces cerevisiae and bacillus subtilis which are independently screened and optimized in a laboratory as fermentation strains, performing fermentation under the optimal fermentation condition and the natural pH condition, and after the fermentation is finished, performing low-temperature drying to obtain the fermented mushroom bran feed. The nutritional ingredients and feed quality of the mushroom bran fermented by mixing the three strains of the bacillus subtilis, the lactobacillus acidophilus and the saccharomyces cerevisiae are remarkably improved, the problems that the mushroom bran is poor in palatability, low in absorption and utilization rate, prone to mildewing, not prone to stable storage and the like are greatly solved, and the mushroom bran is a novel feed resource. The fermented mushroom bran feed is rich in probiotic metabolites, and can maintain the balance of gastrointestinal flora of animals, inhibit the proliferation of bad microorganisms in intestinal tracts, improve the absorption and utilization rate of the feed, enhance the immunity of animal organisms, and improve the health level and production performance of the animals.

Description

technical field [0001] The invention relates to the field of feed, in particular to a method for improving the quality of bacterial chaff feed through multi-strain mixed fermentation. Background technique [0002] China is the world's largest producer of edible fungi. According to incomplete statistics, my country's output of edible fungi has reached 39.6 million tons in 2019, accounting for more than 75% of the world's total output of edible fungi. Mushroom chaff is the residue of the culture medium left after the cultivation of various fungi, which contains protein, amino acid and other nutrients. It is estimated that the annual output in my country is about 40 million tons. With the development of industrialization of edible fungi, the output of edible fungi has grown rapidly, and the number of mushroom chaff has also increased year by year. At present, only a small part of these fungus chaffs are developed and utilized as organic fertilizers or cultivation substrates, a...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/37C12N1/20C12N1/18C12R1/865C12R1/125C12R1/23
CPCA23K10/12A23K10/37C12N1/20C12N1/18Y02P60/87
Inventor 丁丽孙中超杨旭高小创刘妍夏志生
Owner 上海源耀农牧科技有限公司
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