Leptin receptor affinity peptide and application thereof

Abstract

The invention discloses an Leptin receptor affinity peptide and an application thereof. The sequence of the Leptin receptor affinity peptide is as shown in SEQ ID No.1. The invention further discloses an affinity peptide with collagen binding capacity. The affinity peptide comprises the Leptin receptor affinity peptide and a short peptide with specific collagen binding capacity, and the short peptide with the specific collagen binding capacity is bound to the N end of the Leptin receptor affinity peptide. The affinity peptide has high affinity with a Leptin receptor and can be specifically bound with MSC, the affinity peptide is connected to a biological material, and the affinity peptide connected with the short peptide with the specific collagen binding capacity can achieve specific binding with a collagen scaffold, so that the recruitment of the biological material on the MSC with the surface expressing the Leptin receptor is improved, a better tissue damage repair effect is obtained, and an experimental basis is provided for researching the MSC in the aspects of tissue engineering repair and targeted therapy research.

Description

technical field

[0001] The invention relates to a Leptin receptor affinity peptide, in particular to a Leptin receptor affinity peptide capable of specifically binding to mesenchymal stem cells (MSCs) and a screening method thereof, which is applied to tissue engineering repair and belongs to biomedical technology field. Background technique

[0002] Mesenchymal stem cells (MSCs), mainly derived from mesoderm, are a type of adult stem cells with high self-renewal ability and differentiation potential, and are important members of the stem cell family. MSCs were first discovered in the bone marrow, and later studies found that MSCs mainly exist in the connective tissue of the whole body and the interstitium of organs. At present, MSCs can be isolated and prepared from bone marrow, placenta, umbilical cord, fat and other tissues. Based on the multi-differentiation potential of MSC, it can be directed to differentiate into bone, cartilage, fat, cardiac muscle and other tissue ...

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