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Method for separating, extracting and purifying dogwood seed polysaccharide

A technology of dogwood seed and purification method, which is applied in the field of separation, extraction and purification of dogwood seed polysaccharides, can solve the problems of waste of resources, short preparation cycle, damage to the structure of dogwood seed, etc., and achieve the effect of improving purity and increasing surface area

Inactive Publication Date: 2021-08-20
HUBEI POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The overall preparation cycle of the method is short, and the structure of cornus officinalis is destroyed in a short time by subcritical reaction, so that polysaccharides and protein macromolecules are dissolved to a greater extent, and then high-purity cornel officinalis is obtained by deproteinization and column chromatography Seed polysaccharides, thereby effectively solving the situation of wasting resources of cornus officinalis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for separation, extraction and purification of Cornus officinalis polysaccharide of the present embodiment comprises the following steps:

[0024] After drying the cornel seed to a water content of 10%, crush it until the powder can pass through an 80-mesh sieve, degrease it with petroleum ether for 3 times, evaporate the petroleum ether to obtain the defatted cornel seed powder; Milliliters of distilled water, add distilled water to the degreased Cornus officinalis seed powder, place in a subcritical reaction kettle, react at a temperature of 160°C for 20min, then rapidly cool to room temperature, and suction filter to obtain the extract and filter residue;

[0025] According to the dosage ratio of activated carbon to extract 1g: 50mL, add activated carbon to the extract, shake at a constant temperature of 25°C for 1 hour at a speed of 200r / min, so that the activated carbon is evenly dispersed in the extract, thereby removing the colored Impurities are adsorbe...

Embodiment 2

[0030] A method for separation, extraction and purification of Cornus officinalis polysaccharide of the present embodiment comprises the following steps:

[0031] After drying cornel seeds to a water content of 8%, crush them until the powder can pass through an 80-mesh sieve, degrease with petroleum ether for 3 times, and evaporate the petroleum ether to obtain defatted cornel seed powder; Milliliters of distilled water, add distilled water to the defatted Cornus officinalis seed powder, place in a subcritical reaction kettle, react at a temperature of 120°C for 60min, then rapidly cool to room temperature, and suction filter to obtain the extract and filter residue;

[0032] According to the dosage ratio of activated carbon to extract 1g: 40mL, add activated carbon to the extract, and shake at a constant temperature of 30°C for 1.5h at a rate of 100r / min, so that the activated carbon is evenly dispersed in the extract, so that the extract in the extract The colored impuritie...

Embodiment 3

[0037] A method for separation, extraction and purification of Cornus officinalis polysaccharide of the present embodiment comprises the following steps:

[0038] After drying the cornel seed to a water content of 9%, crush it until the powder can pass through an 80 mesh sieve, degrease it with petroleum ether for 3 times, evaporate the petroleum ether to obtain the defatted cornel seed powder; Milliliters of distilled water, add distilled water to the degreased Cornus officinalis seed powder, place in a subcritical reaction kettle, react at a temperature of 200 ° C for 3 minutes, then rapidly cool to room temperature, and suction filter to obtain the extract and filter residue;

[0039] According to the dosage ratio of activated carbon to extract 1g: 60mL, add activated carbon to the extract, and shake at a constant temperature of 40°C for 0.5h at a rate of 300r / min, so that the activated carbon is evenly dispersed in the extract, so that the extract in the extract The colore...

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Abstract

The invention discloses a method for separating, extracting and purifying dogwood seed polysaccharide, which belongs to the technical field of dogwood seed polysaccharide extraction, and comprises the steps of drying dogwood seeds, crushing, degreasing, uniformly dispersing in a water solvent, reacting in a subcritical reaction kettle, and carrying out suction filtration to obtain an extracting solution; removing colored impurities in the extracting solution by using activated carbon to obtain a clear crude polysaccharide solution, then adding a Sevage reagent, enabling the Sevage reagent to be uniformly dispersed in the crude polysaccharide solution, standing, and collecting supernate to obtain a deproteinized polysaccharide solution; performing vacuum concentration on the deproteinized polysaccharide solution, and performing vacuum freeze drying to obtain a dogwood seed polysaccharide crude product; and eluting the crude dogwood seed polysaccharide product by using a DEAE-52 cellulose column and a Sephadex G-100 column in sequence, and carrying out vacuum freeze drying on the eluent to obtain a pure dogwood seed polysaccharide product. The whole separation, extraction and purification time is short, the obtained dogwood seed polysaccharide is high in purity and suitable for large-scale production, and the problem of waste of dogwood seed resources is effectively solved.

Description

technical field [0001] The invention relates to the technical field of extracting plant polysaccharides, in particular to a method for separating, extracting and purifying cornel seed polysaccharides. Background technique [0002] Cornus officinalis, also known as cornus and jujube, is a small deciduous tree of the family Cornus officinalis, mainly distributed in Zhejiang, Henan, Shandong and other places in my country. Clinically, its dried and ripe pulp is used as medicine, which has the effects of nourishing the liver and kidney, astringent essence and detoxification. It is a traditional Chinese medicinal material in my country. [0003] In recent years, due to the rich sugars, organic acids, esters, tannins, ketones and other medicinal and nutritional health ingredients in the flesh of Cornus officinalis, it is the main medicinal material for treating diabetes, coronary heart disease and hypertension. And studies have shown that Cornus officinalis polysaccharide is an i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 詹亦贝
Owner HUBEI POLYTECHNIC UNIV