Transplanting method of conidia of magnaporthe oryzae

Abstract

The invention relates to a transplanting method of conidia of magnaporthe oryzae. The conidia are adsorbed from a spore-producing bacterial colony and are directly transplanted to an observation plate, and the operation steps of conidia transplanting are as follows: (1) preparing pathogen spores; (2) sterilizing tools; (3) preparing the observation plate; (4) adsorbing and diluting the spores; (5) releasing the spores; and 6) performing culturing and observing. The transplanting method has the advantages that (1) a key working material (the conidia of the magnaporthe oryzae) do not need to be made into a spore suspension, so that the pollution risk is reduced; (2) the conidia of the pyricularia oryzae can be dispersed on the strip-shaped culture plate, and the germination and growth activities of the conidia of the pyricularia oryzae are observed, so that the efficiency and the effect of observation work are improved, and development of multi-factor action design and batch test comparison in spore germination biological research is facilitated.

Description

technical field [0001] The invention relates to plant pathology technology, in particular to a method for transplanting conidia of rice blast fungus. Background technique [0002] Rice blast is an important disease of rice production in my country, which can cause heavy losses to rice production, even complete loss of harvest. The disease is caused by the infection of Magnaporthe oryzae. Conidia are the asexual propagules of Magnaporthe grisea, which play an important role in the disease cycle and epidemic of Magnaporthe grisea. A full understanding of the germination biology of conidia is crucial to effectively control the occurrence and epidemic of rice blast. In the germination biology of rice blast fungus vegetative body and related research work, it is often necessary to transplant conidia to the culture plate for observation and culture. This observation and culture not only requires the spores on the culture plate to be in a proper dispersed state, but also The numb...

AI Technical Summary

Problems solved by technology

This method seems simple, but there are still considerable difficulties and tediousness in practical operation, mainly in that in the technical operation of obtaining the key working material (conidia of rice blast fungus), it is often necessary to take filtration operation to remove the spores The hyphae in the spore liquid also need to be centrifuged to remove the medium and culture metabolites in the spore liquid. These operations have a greater risk of contamination. In addition, the spore concentration of the spore liquid needs to be adjusted to an appropriate range. , so it needs to be repeatedly prepared and sampled to the counting plate or glass slide, and it is also transferred to the biological microscope for observation and counting, which is cumbersome

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