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Phenolic compound analysis method based on polyphenol oxidase active nano enzyme

A technology of phenolic compounds and polyphenol oxidase, which is applied in the direction of material analysis by observing the influence on chemical indicators, biochemical equipment and methods, and analysis by making materials undergo chemical reactions, can solve the problem of high detection cost, Time-consuming, long detection time and other problems, to achieve the effect of high detection sensitivity, strong scalability, and high accuracy

Active Publication Date: 2021-08-24
JILIN UNIV
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Problems solved by technology

However, the above method has huge equipment, high detection cost, and long time-consuming. It requires complex pretreatment processes such as extraction and separation of samples, requires professional personnel to operate, requires a lot of manpower and material resources, long detection time, expensive instruments, and maintenance by professional technicians. , the detection cost is high, it is not suitable for on-site detection, and cannot meet the detection needs of a wide variety of agricultural products

Method used

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  • Phenolic compound analysis method based on polyphenol oxidase active nano enzyme
  • Phenolic compound analysis method based on polyphenol oxidase active nano enzyme
  • Phenolic compound analysis method based on polyphenol oxidase active nano enzyme

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preparation example Construction

[0026] (1) Preparation of the array sensor: a colorimetric array was constructed by combining the laccase properties of the nanozyme with nucleotides and copper coordination, and each sensing unit was composed of a reaction time and an optimal wavelength. Through the calculation of chemometrics algorithm - genetic algorithm, the reaction system has 5 optimal response wave points in the whole band, and the response time constitutes each sensing unit of the array sensor of wavelength × time (5 × 3), so as to obtain Sensor array with multiple sensing units.

[0027] (2) Discriminant analysis of different phenolic pollutants: the aqueous solution of phenolic substances is added to the array sensor array described in step (1), and mixed with the detection solution (enzyme substrate, which generates a colorimetric signal) therein to generate Color reaction, read the ultraviolet absorption value of the detection solution through the instrument, and digitally process the color develop...

Embodiment 1

[0038] Embodiment 1 realizes the recognition and distinction to multiple phenolic pollutants

[0039] Make reaction system according to embodiment (1), react 30min, 60min, 120min.

[0040] Sensing unit 1: The characteristic spectra when reacting for 30 minutes are 383, 394, 398, 414, 465nm respectively.

[0041] Sensing unit 2: The characteristic spectra when reacting for 60 minutes are 360nm, 366, 390, 495, 498nm respectively.

[0042] Sensing unit 3: The characteristic spectra when reacting for 120 minutes are 377, 381, 407, 421, 509nm respectively.

[0043] Put a certain volume of the reaction system configured in embodiment (1) into a 96-well plate, read the absorbance value under the sensing unit of the array sensor for detecting phenolic substances through a microplate reader, and run the experiment in parallel for 10 Second, the obtained data was imported into MATLAB software for partial least squares discriminant analysis, and the score map of the principal component...

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Abstract

The invention belongs to the technical field of phenol detection, and particularly relates to a phenolic compound analysis method based on polyphenol oxidase active nano enzyme, which comprises the following steps: (1) preparing an array sensor; (2) performing discriminant analysis of different phenol pollutants; (3) performing discriminant analysis of different concentrations of the same phenolic substance; (4) performing discriminant analysis after the two phenolic substances are mixed; and (5) performing discriminant analysis of phenolic substances in an actual sample. According to the phenolic compound analysis method based on the polyphenol oxidase active nano-enzyme, an array sensor is constructed based on a nano-enzyme system, the detection sensitivity is high, the expandability is high, different phenolic pollutants are distinguished and analyzed, the specificity is high, the accuracy is high, a colorimetric array is combined with a chemometrics algorithm, the sensor is constructed by utilizing the characteristic wavelength, so that the establishment of the sensor has a theoretical basis.

Description

technical field [0001] The invention relates to the technical field of phenol detection, in particular to a method for analyzing phenol compounds based on polyphenol oxidase activity nanozyme. Background technique [0002] Phenolic compounds are the basic raw materials in industrial and agricultural production, which are highly toxic and difficult to degrade, and most phenolic pollutants have high solubility in water, which increases their migration ability in water bodies, making them gradually become One of the main pollutants in water, so it is of vital significance for the detection of phenolic pollutants in water. At present, the methods for detecting phenolic substances in water mainly include chromatography, spectrophotometry, fluorescence analysis and electrochemical analysis. However, the above method has huge equipment, high detection cost, and long time-consuming. It requires complex pretreatment processes such as extraction and separation of samples, requires pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/33C12Q1/26
CPCG01N21/78G01N21/33C12Q1/26
Inventor 李永新杨晓玉张雯婧黄卉孙悦宋冬辉
Owner JILIN UNIV
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