ALK fusion gene detection and typing kit based on sandwich method high-resolution melting curve analysis

A technology of fusion genes and kits, which is applied in the determination/inspection of microorganisms, recombinant DNA technology, biochemical equipment and methods, etc., and can solve the difficulties of various fusion gene types, mixed interstitial cell components, and frequent changes in fusion gene sequences to achieve the effects of low pollution risk, fast detection speed, and short detection cycle
CN109628599AActive Publication Date: 2019-04-16THE SECOND HOSPITAL OF DALIAN MEDICAL UNIV
5 Cites 1 Cited by

Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
THE SECOND HOSPITAL OF DALIAN MEDICAL UNIV
Publication Date
2019-04-16

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses an ALK fusion gene detection and typing kit based on sandwich method high-resolution melting curve analysis. A sandwich sequence target segment is amplified in a segmented manner by adding a low GC content joint at the 5' end of a gene-specific primer, and is a fusion gene fragment comprising a high and low GC content consensus sequence and a variable sequence. A high-resolution melting curve general analysis method can judge presence or absence of the fusion gene, and the fluorescence intensity-temperature second-order derivative curve is combined with a key parameteranalysis method to achieve digital judgment of the fusion gene type. The kit solves the problems of high cost, long cycle and difficult typing of the existing lung cancer ALK fusion gene detection, integrates the advantages of two technologies of real-time quantitative reverse transcription PCR and high-resolution melting curve analysis, and can be applied to fusion gene detection of fresh tissues, paraffin-embedded tissues, pleural effusion and other types of specimens.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of in vitro detection, and in particular relates to a kit for amplifying, detecting and typing common types of ALK fusion genes in lung cancer. Background technique

[0002] Lung cancer is a malignant tumor with high morbidity and mortality, and its incidence is increasing year by year in our country. The emergence of individualized molecular targeted therapy has made up for the shortcomings of surgical treatment and traditional chemotherapy, and the quality of life and survival period of such drug-sensitive patients have been significantly improved. At present, lung cancer molecular targeted drug tyrosine kinase inhibitor (TKI) mainly targets two types of gene mutations, one is point mutations and deletion mutations of EGFR and other genes, and the other is fusion genes. Gene point mutations and deletion mutations are relatively easy to detect, but fusion genes in solid tumors such as lung cancer are diffi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More