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In-situ screening and complex microbial inoculant preparation method for degrading chlorophenol flora

A technology of composite bacterial agent and chlorophenol bacteria, which is applied in the direction of using microorganisms, bacteria, and separating microorganisms, can solve problems such as uneven distribution of bacteria, affecting bacterial treatment, and insufficient extraction of bacteria, so as to improve stability and reduce The effect of small repulsion

Pending Publication Date: 2021-08-27
SUZHOU WINNER ENVIRONMENTAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, during the preparation of the existing chlorophenol-degrading bacteria group, due to the uneven distribution of the bacteria and the uniform distribution in the sludge, the extraction of the bacteria will not only easily lead to insufficient extraction of the bacteria, but also lead to difficulty in the extraction of the bacteria. large, which seriously affects the treatment of bacteria. Therefore, we propose a method for in-situ screening of chlorophenol-degrading bacteria and preparation of compound bacteria

Method used

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  • In-situ screening and complex microbial inoculant preparation method for degrading chlorophenol flora

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Embodiment 1

[0042] A kind of in situ screening of degrading chlorophenol flora, comprising the following steps:

[0043] Raw material screening: select the sludge with anaerobic particles, stir in vacuum, and then extrude into raw material particles with a diameter within 5mm, pour the sludge and agar into the vacuum mixer for stirring, and pour into granulation after stirring Machine-made raw material pellets:

[0044] Raw material cultivation: apply the culture medium evenly on the outside of the screened raw material granules, and use a hot air blower to heat the raw material granules, and wait until the medium wraps the raw material granules to obtain anaerobic raw materials. The following steps are included when smearing the medium on the surface of the raw material granules:

[0045] Preliminary smearing: first smear agar particles with a diameter within the range of 2mm, and then use a hot air blower to heat the outside of the agar particles at a temperature of 50°C. After heating ...

Embodiment 2

[0055] A kind of in situ screening of degrading chlorophenol flora, comprising the following steps:

[0056] Raw material screening: select the sludge with anaerobic particles, stir in vacuum, and then extrude into raw material particles with a diameter within 5mm, pour the sludge and agar into the vacuum mixer for stirring, and pour into granulation after stirring Machine-made raw material pellets:

[0057] Raw material cultivation: apply the culture medium evenly on the outside of the screened raw material granules, and use a hot air blower to heat the raw material granules, and wait until the medium wraps the raw material granules to obtain anaerobic raw materials. The following steps are included when smearing the medium on the surface of the raw material granules:

[0058] Preliminary smearing: first smear agar particles with a diameter within the range of 2mm, and then use a hot air blower to heat the outside of the agar particles at a temperature of 50°C. After heating ...

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Abstract

The invention discloses an in-situ screening and complex microbial inoculant preparation method for degrading a chlorophenol flora. The method comprises the steps of raw material screening, raw material culture, raw material domestication, bacterium extraction and bacterium secondary screening. According to the in-situ screening and complex microbial inoculant preparation method for degrading the chlorophenol flora, firstly, when raw materials are cultured, the surfaces of the raw materials are coated with a culture medium, and the raw materials are heated by a hot-air blower so that a sealed shell can be formed on the surfaces of raw material particles, bacteria can be kept in an oxygen-free environment all the time, and the bacteria can be cultured; secondly, multiple layers of culture media are arranged on the outer sides of the raw material particles, bacteria can be attracted to the surfaces of the particles, the bacteria are intensively distributed on the surfaces of the raw material particles, and subsequent extraction and screening are facilitated; and thirdly, when the microbial agents are mixed, dripping mixing is adopted so that rejection among bacteria can be reduced, and the mixing stability among the microbial agents is improved.

Description

technical field [0001] The invention relates to the technical field of chlorophenol degradation, in particular to a method for in-situ screening of chlorophenol-degrading bacteria and preparation of a composite bacterial agent. Background technique [0002] Chlorophenol is the general term for chlorine-substituted phenol compounds. The basic structure is that chlorine replaces the hydrogen atoms on the benzene ring. According to the position and number of chlorine atoms substituted, various chlorophenols with similar structures and properties are formed. A large number of environmental investigations and experiments Studies have shown that chlorophenol can enter the body through oral or skin contact. Long-term contact or ingestion can cause skin itching, rash, dizziness, anemia and various nervous system diseases. Excessive ingestion can cause acute poisoning. Mutagenicity and carcinogenicity; [0003] At present, during the preparation of the existing chlorophenol-degradin...

Claims

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Application Information

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IPC IPC(8): C12N1/36C12N1/02C12N1/20
CPCC12N1/02C12N1/20C12N1/36
Inventor 王玲张海芹李小平
Owner SUZHOU WINNER ENVIRONMENTAL TECH