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Amino acid production method

A production method and technology of glutamic acid, applied in the field of microbial fermentation, can solve the problems of complex corn steep liquor composition, large fluctuation of corn steep liquor fermentation index, and little attention to the removal of harmful substances of corn steep liquor, etc. The effect of specific function and low equipment requirements

Inactive Publication Date: 2021-08-31
MEIHUA BIOTECH LANGFANG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] As a cheap organic nitrogen source, corn steep liquor has been widely used in the amino acid fermentation industry. However, due to the complex composition of corn steep liquor, there are many problems in the actual application process.
The composition of corn steep liquor is affected by different sources of corn and the soaking process, and the content of toxins and other harmful substances in corn under different storage conditions will also change, resulting in large fluctuations in the fermentation index of corn steep liquor
Current research mainly focuses on the utilization of nutrients in corn steep liquor. By chemical acid hydrolysis or biological enzymatic pretreatment, the unusable macromolecular proteins in corn steep liquor can be degraded into small molecular amino acids that can be used by microorganisms, or phytic acid and other organic Phosphorus is converted to inorganic phosphorus, but little attention has been paid to the removal of substances harmful to microbial fermentation in corn steep liquor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] The present embodiment provides a kind of glutamic acid production method, comprises the steps:

[0059] (1) Corn steep liquor pretreatment: Take 500mL corn steep liquor and put it into a 2L Erlenmeyer flask with a dry matter content of 40%, use sodium hydroxide to adjust the pH value of corn steep liquor to 7.0, add xanthine according to the amount of 50 μg / kg corn steep liquor (dry basis) Oxidase, placed in a constant temperature shaker at 50°C for 30 minutes;

[0060] (2) Preparation of fermentation medium: use pretreated corn steep liquor for the preparation of glutamic acid fermentation medium, the dosage of pretreated corn steep liquor is 30g / L, the dosage of other components is 50g / L of glucose, and 30g of corn steep liquor hydrolyzate / L,K 2 HPO 4 3g / L, MgSO 4 ·7H 2 O 1.5g / L, biotin 400μg / L, FeSO 4 ·7H 2 O 10mg / L, MnSO 4 ·H 2 O 10mg / L, according to the volume of the fermenter, add it to a 10L fermenter at a constant volume according to 60% of the liquid...

Embodiment 2

[0063] The present embodiment provides a kind of glutamic acid production method, comprises the steps:

[0064] (1) Corn steep liquor pretreatment: Take 500mL corn steep liquor and put it into a 2L Erlenmeyer flask with a dry matter content of 40%, adjust the pH value of corn steep liquor to 7.0 with sodium hydroxide, add xanthine according to the amount of 100 μg / kg corn steep liquor (dry basis) Oxidase, placed in a constant temperature shaker at 55°C for 30 minutes;

[0065] (2) Preparation of fermentation medium: use pretreated corn steep liquor for the preparation of glutamic acid fermentation medium, the dosage of pretreated corn steep liquor is 40g / L, the dosage of other components is 50g / L of glucose, and 30g of corn steep liquor hydrolyzate / L,K 2 HPO 4 3g / L, MgSO 4 ·7H 2 O 1.5g / L, biotin 400μg / L, FeSO 4 ·7H 2 O 10mg / L, MnSO 4 ·H 2 O 10mg / L, according to the volume of the fermenter, add it to a 10L fermenter at a constant volume according to 60% of the liquid ...

Embodiment 3

[0068] The present embodiment provides a kind of glutamic acid production method, comprises the steps:

[0069] (1) Corn steep liquor pretreatment: Take 500mL corn steep liquor and put it into a 2L Erlenmeyer flask with a dry matter content of 40%, adjust the pH value of corn steep liquor to 7.5 with sodium hydroxide, add xanthine according to the amount of 150 μg / kg corn steep liquor (dry basis) Oxidase, placed in a constant temperature shaker at 55°C and shaken for 60 minutes;

[0070] (2) Preparation of fermentation medium: use pretreated corn steep liquor for the preparation of glutamic acid fermentation medium, the dosage of pretreated corn steep liquor is 50g / L, the dosage of other components is 50g / L of glucose, and 30g of corn steep liquor hydrolyzate / L,K 2 HPO 4 3g / L, MgSO 4 ·7H 2 O 1.5g / L, biotin 400μg / L, FeSO 4 ·7H 2 O 10mg / L, MnSO 4 ·H 2 O 10mg / L, according to the volume of the fermenter, add it to a 10L fermenter at a constant volume according to 60% of ...

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Abstract

The invention relates to the field of microbial fermentation, in particular to an amino acid production method. When the corn steep liquor pretreated by the method is applied to microbial fermentation, the fermentation effect is better. According to the production method provided by the invention, the sugar-acid conversion rate is further improved, which is beneficial to popularization and application in glutamic acid production.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to an amino acid production method. Background technique [0002] Corn steep liquor is a by-product obtained by concentrating the soaking water produced in the corn wet soaking production process. It has complex components and is rich in nutrients such as protein, amino acids, vitamins (such as biotin, etc.), nucleotides, and trace elements. Corn steep liquor is an excellent nitrogen source because it is cheap and easy to obtain, and has been widely used in the production process of many fermentation products such as amino acids, antibiotics, and biochemical drugs. According to the utilization degree of microorganisms, the components in corn steep liquor can be divided into fermentable components and non-fermentable components. Fermentable components include amino acids, vitamins and growth factors, etc. Fermentable components can promote the growth of microorganisms and the sy...

Claims

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Application Information

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IPC IPC(8): C12P13/14C12N1/20C12R1/15
CPCC12N1/20C12P13/14
Inventor 李必金李栋梁宫仔龙李岩
Owner MEIHUA BIOTECH LANGFANG CO LTD