Method for simultaneously determining contents of three active components of ancient classical famous formula compound preparation Juanbi granules

Abstract

The invention discloses a method for simultaneously determining the contents of three active components of Juanbi granules as an ancient classical famous formula compound preparation. The chromatographic conditions adopted by the method are as follows, a chromatographic column is AgilentZORBAX SB-C18 (250mm*4.6 mm, 5 [mu] m), acetonitrile and 0.1% formic acid are used for gradient elution, the flow velocity is 1.0 ml.min<-1>, the detection wavelengths are as follows, chlorogenic acid and ferulic acid are 320nm, gentiopicroside is 277nm, the column temperature is 30 DEG C, and the sample size is 10 [mu] l. Under the chromatographic condition, the linear ranges of chlorogenic acid, gentiopicroside and ferulic acid are 9.848 to 59.088 [mu] g.ml<-1> (R is equal to 0.99895), 49.588 to 297.528 [mu] g.ml<-1> (R is equal to 0.99904) and 6.016 to 36.096 [mu] g.ml<-1> (R is equal to 0.9976) respectively, and the average sample recovery rates are 101.4% (RSD is equal to 2.4%), 97.2% (RSD is equal to 2.7%) and 100.0% (RSD is equal to 2.7%) (n is equal to 6) respectively. Chromatographic peaks of chlorogenic acid, gentiopicroside and ferulic acid are well separated, and concentration peak areas are in a good linear relationship. The method is simple, rapid, accurate and good in reproducibility, and a theoretical basis can be provided for comprehensively evaluating and controlling the quality of Juanbi granules as an ancient and classical famous formula compound preparation.

Description

technical field [0001] The invention relates to a method for detecting three active ingredients in Juanbi Granules, an ancient classic famous prescription compound preparation of a new traditional Chinese medicine, specifically a method for simultaneously measuring the contents of three active ingredients in Juanbi Granules, an ancient classic famous prescription compound preparation. It belongs to the field of medical technology. Background technique [0002] Juanbi granule, an ancient classic famous prescription compound preparation of new traditional Chinese medicine, dispels wind and dampness, Juanbi relieves pain, and mainly treats numbness caused by the combination of wind, cold and dampness. Arthralgia in traditional Chinese medicine, modern Chinese medicine calls it 尪bi, refers to the joint disease caused by wind, cold and dampness attacking the joints, qi and blood blockage, characterized by small joint pain, swelling, morning stiffness, and even deformation, the di...

AI Technical Summary

Problems solved by technology

[0003] At present, there are few domestic studies on the quality control of Juanbi granule preparations of ancient classic famous prescriptions of new traditional Chinese medicines. In order to effectively control the quality of the preparations, chlorogenic acid, gentiopicroside, and ferulic acid are selected as the quality of Juanbi granules. Evaluation index to provide scientific basis for controlling the quality of Juanbi Granules

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