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Bacillus subtilis SCUEC7 strain and application thereof

A technology of Bacillus subtilis and strains, applied in the field of microorganisms, to achieve the effects of accelerated fermentation, wide application potential, and fast growth

Active Publication Date: 2021-09-03
JIANGSU SHEN LI ECOLOGICAL AGRI TECHCO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently commonly used hormonal drugs can produce resistance to inflammation, so it is of great significance to develop new drugs that can relax the airway
At present, there is no relevant research report on the application of Bacillus subtilis in the diastolic tracheal ring.

Method used

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  • Bacillus subtilis SCUEC7 strain and application thereof
  • Bacillus subtilis SCUEC7 strain and application thereof
  • Bacillus subtilis SCUEC7 strain and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Example 1 Directional enrichment isolation and identification of Bacillus subtilis SCUEC7 strain

[0026] 1. Directional enrichment and isolation of SCUEC7 strain

[0027] Mix the gastric juice taken from the rumen of a healthy cow with the crop straw and ferment for 48 hours, take 10 g of the fermented product, add 90 mL of CMC-Na liquid medium (CNB) with a pH of 5.0, enrich and cultivate at 37°C for 12 hours, and use a 10-fold gradient with sterile water Serial dilution (10 -1 ~10 -5 ), spread 200 μL of the diluted solution evenly on a CMC-Na solid medium (CNA) plate, incubate at 37°C for 24 hours, add 0.2% Congo red staining solution to stain for 30 minutes, and then wash off thoroughly with distilled water and 1mol / L NaCl solution successively. For the dye solution, colonies with larger degradation circles were picked, separated and purified by streaking multiple times, and multiple strains of microorganisms with larger degradation circles were screened. Among th...

Embodiment 2

[0038] The growth of embodiment 2 bacillus subtilis SCUEC7 bacterial strain and produce endoglucanase activity

[0039] Inoculate the SCUEC7 strain seed liquid into the CMC-Na liquid medium (CNB) with a volume ratio of 0.5% inoculum, cultivate with shaking at 37 ° C, and take samples on time to measure the absorbance value of the bacterium liquid at 600 nm as the growth amount of the bacterium. Simultaneously measure the endoglucanase activity produced by it, wherein the definition of endoglucanase enzyme activity is defined according to the international unit: the amount of enzyme required to catalyze the hydrolysis of CMC-Na to produce 1 μg of glucose per minute is defined as an enzyme activity unit (U), the result is as figure 1 Shown: OD of SCUEC7 strain at 0-12h 600 From 0.02 to 3.69, endoglucanase activity increased from 2.27U / mL to 239.98U / mL. At 12-24h, the OD of SCUEC7 strain 600 Between 3.51 and 3.69, the enzyme activity is between 239.98U / mL and 218.10U / mL. The ...

Embodiment 3

[0040] The pathogen inhibitory activity of embodiment 3 bacillus subtilis SCUEC7 bacterial strain

[0041] 1. Antibacterial activity of SCUEC7 strain against plant pathogenic bacteria

[0042] Botrytis cinerea, Pythium spp., Fusarium wilt of watermelon, Early blight of tomato, Morus rotifera and Fusarium graminearum were used as indicator bacteria to study the antibacterial activity of SCUEC7 strain against different plant pathogens.

[0043] Take the Bacillus subtilis SCUEC7 strain, culture it on CNB medium at 37°C for 12 hours to obtain the culture solution, then centrifuge the culture solution at 8000r / min for 10min, take 5μL of the culture supernatant for the antibacterial experiment, and measure the diameter of the inhibition zone , and with the conventional Bacillus subtilis ATCC6633 bacterial strain as a contrast, the measurement results are as shown in Table 2: the supernatant of Bacillus subtilis SCUEC7 bacterial strain is effective against botrytis cinerea, pythium, ...

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Abstract

The invention discloses a bacillus subtilis SCUEC7 strain and application thereof. The bacillus subtilis SCUEC7 strain has been deposited in China Center for Type Culture Collection on April 15, 2021, and the accession number is CCTCC NO: M 2021377. The SCUEC7 strain provided by the invention is isolated from a mixed fermentation product of cattle gastric juice and crop straw, has an inhibition effect on various plant pathogens such as botrytis cinerea and pythium ultimum, can be used for preventing and treating crop diseases, also has an inhibition effect on pathogenic bacteria such as staphylococcus aureus, and can be used for production of foods, feeds and the like; meanwhile, the SCUEC7 strain can also promote growth of candida utilis, and by applying the SCUEC7 strain to feed production, feed fermentation can be accelerated, and the content of single-cell protein of fermented feed is effectively increased; and moreover, It is the first time for the invention to discover that the bacillus subtilis SCUEC7 strain has a relaxation effect on a mouse acetylcholine pre-contracted tracheal ring, and therefore, the bacillus subtilis SCUEC7 strain can be prepared into a tracheal ring relaxation drug for treating diseases such as asthma.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a strain of Bacillus subtilis SCUEC7 and an application thereof. Background technique [0002] Bacillus subtilis (Bacillus subtilis) is a kind of Bacillus genus, which is widely distributed in soil and decaying organic matter, and is easy to reproduce in the withered grass juice, so it gets its name. The bacterium is uniformly colored, has no capsule, has flagella, can move, and is positive for Gram staining. The surface of the colonies is rough and opaque, dirty white or yellowish, and they are aerobic bacteria. It can use protein, various sugars and starch to decompose tryptophan to form indole. Bacillus subtilis can produce endophytic spores, which are heat-resistant and stress-resistant. Because Bacillus subtilis grows fast, has simple nutritional requirements, is easy to survive, colonize and reproduce, is non-pathogenic, and can secrete a variety of en...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P1/00C12N9/24C12N1/16A23K10/12A61K35/742A61P11/06C12R1/125C12R1/72
CPCC12N1/20A01N63/22C12N9/2405C12N1/16A61K35/742A61P11/06A23K10/12Y02P60/87
Inventor 王力李晓华张建国许琪寻立之冯喆王圣心赵思卡杨佳琪许倍滔
Owner JIANGSU SHEN LI ECOLOGICAL AGRI TECHCO LTD
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