A kind of preparation method of biofilm and its product and application

Abstract

The invention provides a preparation method of a biofilm and its product and application. The preparation method of the biofilm comprises the following steps: (1) using a collagenase inhibitor to treat the animal cavity tissue; (2) removing the film layer accounting for more than 40% of the total fat content of the animal cavity tissue; (3) using the first The product obtained in the enzyme solution treatment step (2); (4) the product obtained in the alkali solution treatment step (3); (5) the product obtained in the decellularization solution treatment step (4); (6) the degreasing solution treatment The product obtained in step (5); (7) using the second enzyme solution to treat the product obtained in step (6); (8) cleaning the product obtained in step (7) with supercritical carbon dioxide to obtain a biofilm. The invention can largely retain the integrity of the multi-layer space structure of the biofilm, the elastic fiber and the collagen network structure, has high mechanical strength, can remove the residues of organic matter and reagents in the product, and has a better effect of inducing tissue regeneration .

Description

A kind of preparation method of biofilm, product and application thereof technical field The present invention relates to biotechnology field, be specifically related to a kind of preparation method of biofilm and its product and application, especially It relates to the preparation of a multi-layered natural biofilm based on animal cavity tissue and its application in guiding tissue regeneration. use. Background technique The progress and development of tissue engineering technology are reconstruction of urinary system, reconstruction of digestive system, reconstruction of female reproductive tract, etc. The repair of vital tissues and organs offers hope. Tissue engineering technology includes scaffold materials, seed cells and growth factors. area. The extracellular matrix is ​​a good scaffold material, and the collagen network structure and elastic fibers contained in the extracellular matrix are conducive to thinning. cell adhesion growth, has a good clinical...

AI Technical Summary

Problems solved by technology

This method also has the problems of excessive drug dosage, incomplete decellularization, high drug toxicity and low mechanical properties of the preparation materials in the way of decellularization and degreasing treatment; Extremely long (it takes 45 days to complete the process to prepare)

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