Preparation method of biological membrane as well as product and application of biological membrane

A biofilm and animal technology, which is applied in tissue regeneration, medical science, surgery, etc., can solve the problems of large dosage of drugs, large residues, and incomplete decellularization

Active Publication Date: 2021-09-10
WUQI BIOMEDICAL TECH (JIANGSU) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method also has the problems of excessive drug dosage, incomplete decellularization, high drug toxicity and low mechanical properties of the preparation materials in the way of decellularization and degreasing treatment; Extremely long (it takes 45 days to complete the process to prepare)

Method used

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  • Preparation method of biological membrane as well as product and application of biological membrane
  • Preparation method of biological membrane as well as product and application of biological membrane
  • Preparation method of biological membrane as well as product and application of biological membrane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0100] The present embodiment provides a kind of biofilm, and the preparation method of described biofilm comprises the following steps:

[0101] (1) Soak 100g of fresh porcine bladder tissue material in 1L of 10wt% acetylcysteine ​​solution for 2h;

[0102] (2) Cut the product obtained in step (1) at 25°C with a slicing device to remove a film layer with a thickness of 1mm from the bladder material serosa layer, wherein the specific process parameters for mechanical cutting device removal are: voltage 220V, power 3600W, The cutting speed is 15mm / s, and the surface roughness of the material after cutting is less than 100μm;

[0103] (3) Soak 100 g of the product obtained in step (2) in 1 L of 2 wt % trypsin solution at 25° C. for 4 h;

[0104] (4) Soak the product obtained in step (3) at 5° C. for 2 hours in 1 L of a mixed aqueous solution containing 1 mol / L sodium hydroxide and 3% acetylcysteine;

[0105] (5) Soak the product obtained in step (4) at 25°C for 16 hours in 1L ...

Embodiment 2

[0112] This embodiment provides a biofilm, and the preparation method of the biofilm specifically includes the following steps:

[0113] (1) Soak 100g of fresh pig stomach tissue material in 1L of 5wt% sodium citrate solution for 4h;

[0114] (2) The product obtained in step (1) is used at normal temperature to remove a membrane layer with a thickness of 1 mm from the gastric serosa layer using a slicing device, wherein the specific process parameters of mechanical cutting device removal are: voltage 220V, power 3600W, cutting rate 15mm / s, the surface roughness of the material after cutting is <100μm;

[0115] (3) Soak 100 g of the product obtained in step (2) in 1 L of 1 wt% 1398 protease solution at 25° C. for 5 h;

[0116] (4) Soak the product obtained in step (3) at 10° C. in 1 L of 2 mol / L sodium carbonate and 2% sodium citrate mixed aqueous solution for 4 h;

[0117] (5) Soak the product obtained in step (4) at 25°C for 15 hours in 1L of decellularized solution, which ...

Embodiment 3

[0124] This embodiment provides a biofilm, and the preparation method of the biofilm specifically includes the following steps:

[0125] (1) Soak 100 g of fresh bovine large intestine tissue material in 1 L of 15% edetate disodium solution for 2 hours;

[0126] (2) The product obtained in step (1) is used at normal temperature to remove a film layer with a thickness of 3 mm from the serosa layer of the large intestine tissue using a slicing device. The specific process parameters for mechanical cutting device removal are: voltage 220V, power 3600W, cutting The speed is 15mm / s, and the surface roughness of the material after cutting is <100μm;

[0127] (3) Soak 100 g of the product obtained in step (2) in 1 L of 3 wt% trypsin solution at 25° C. for 3 h;

[0128] (4) Soak the product obtained in step (3) in 1 L of 3 mol / L calcium hydroxide and 2% edetate disodium mixed aqueous solution at 15° C. for 1 h;

[0129] (5) Soak the product obtained in step (4) at 15°C for 22 hours i...

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Abstract

The invention provides a preparation method of a biological membrane as well as a product and application of the biological membrane. The preparation method of the biological membrane comprises the following steps: (1) treating animal cavity tissues by adopting a collagenase inhibitor; (2) removing a membrane layer which accounts for more than 40% of the total fat content of animal cavity tissues; (3) treating a product obtained in the step (2) by adopting a first enzyme solution; (4) treating a product obtained in the step (3) by adopting an alkali solution; (5) treating a product obtained in the step (4) by adopting a decellularized solution; (6) treating a product obtained in the step (5) by adopting a degreasing fluid solution; (7) treating a product obtained in the step (6) by adopting a second enzyme solution; and (8) cleaning the product obtained in the step (7) by adopting supercritical carbon dioxide to obtain the biological membrane. The integrity of a multi-layer space structure, elastic fibers and a collagen net structure of the biological membrane can be reserved to a great extent, the mechanical strength is high, residues of organic matters and reagents in the product can be removed, and the tissue regeneration inducing effect of the product is better.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method of a biofilm and its products and applications, in particular to the preparation of a multilayered natural biofilm based on animal cavity tissue and its application in guiding tissue regeneration. Background technique [0002] The advancement and development of tissue engineering technology provides hope for the repair of important tissues and organs such as urinary system reconstruction, digestive system reconstruction, and female reproductive tract reconstruction. Tissue engineering technology includes fields such as scaffold materials, seed cells and growth factors. The extracellular matrix is ​​a good scaffold material. The collagen network structure and elastic fibers contained in the extracellular matrix are conducive to cell adhesion and growth, and have good clinical application prospects. In recent years, many studies have used biological enzymes and re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/00A61L31/14
CPCA61L31/005A61L31/14A61L2430/40A61L2300/412
Inventor 董群任恒飞胡夏练
Owner WUQI BIOMEDICAL TECH (JIANGSU) CO LTD
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