Preparation method for skin-repairing soap containing sashimi collagen
A technology for collagen and skin repair, applied in skin care preparations, medical preparations containing active ingredients, skin diseases, etc. It can solve the problems of unsuitable skin wounds, poor degradation of chemical synthetics, and high pH of soaps, etc. problems, to achieve excellent biocompatibility and biodegradability, easier degradation, and low immunogenicity
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[0019] A preparation method of skin repair soap containing sashimi collagen, comprising the following steps:
[0020] Step 1: Weigh 50g of dried sea cucumber, soak it in water at 60°C for two days, change the water 3 times a day, then extract it in 500mL absolute ethanol at 40°C for two days, change the ethanol once a day, and finally let it dry at room temperature Dry;
[0021] Step 2: Take the dried japonicus japonicus and perform enzymatic hydrolysis reaction with superoxide dismutase for 4 hours. The mass ratio of superoxide dismutase to substrate is 1:6, pH6.5, and temperature 45°C. After the reaction is completed, boil it Inactivate the enzyme for 5 minutes, and quickly cool to room temperature, centrifuge at 2000r / min for 30min, take the supernatant and add 200mL of absolute ethanol to precipitate overnight, then centrifuge at 3000r / min for 30min, take the supernatant and evaporate at 40℃ for 1h to obtain sashimi collagen protein;
[0022] Step 3: Weigh 50g of animal ...
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