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Kimchi for preventing or treating helicobacter pylori-associated diseases

A technology of kimchi and cabbage, which is applied in the field of kimchi containing lactic acid bacteria, and can solve problems such as unfinished

Pending Publication Date: 2021-09-17
CJ CHEILJEDANG CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, research on methods of preventing or treating diseases caused by Helicobacter pylori infection by fermented foods that are frequently consumed such as kimchi has not been completed and is still in progress

Method used

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  • Kimchi for preventing or treating helicobacter pylori-associated diseases
  • Kimchi for preventing or treating helicobacter pylori-associated diseases
  • Kimchi for preventing or treating helicobacter pylori-associated diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] Example 1: Isolation of strains with high Helicobacter pylori bacterial growth inhibition ability

[0090] Isolation and identification of bacterial strains

[0091] Leuconostoc enterococcus CJLM119, which is a lactic acid bacterium derived from kimchi, used in the preparation of the kimchi of the present application is a microbial strain deposited in the Gene Bank of the Korea Institute of Bioscience and Biotechnology under the accession number KCTC 13043BP, and the strain The separation and identification methods of are specifically disclosed in Korean Registered Patent Publication No. 10-1807995. In addition, Lactobacillus plantarum CJLP133, which is a lactic acid bacterium derived from kimchi, used in the preparation of the kimchi of the present application is a microbial strain deposited in the Gene Bank of the Korea Institute of Bioscience and Biotechnology under the accession number KCTC 11403BP, and the strain The isolation and identification methods of are s...

Embodiment 2

[0096] Embodiment 2: the preparation of pickles

[0097] Standard kimchi as a control group (hereinafter, there is a situation expressed as "sKimchi" or "SK") and the kimchi for preventing cancer according to the present application (hereinafter, there is a situation expressed as "CpKimchi" or "CPK") were prepared in the following manner. "Case).

[0098] Preparation of Standard Kimchi (sKimchi)

[0099] Standard kimchi is based on the standardized kimchi recipe of the World Kimchi Research Institute. Seasonings including paprika, garlic, ginger, anchovy paste, radish juice, green onions, glutinous rice paste, and sugar are fermented at low temperature on cabbage pickled in salt. And prepared.

[0100] Preparation of Cancer-preventive Kimchi (CpKimchi)

[0101] Kimchi for cancer prevention uses Chinese cabbage with high lycopene content as the main raw material, and purchases "Han No. 1" (variety application publication number: 2015-688, 2015 It was released on Decem...

Embodiment 3

[0104] Embodiment 3: Determination of the growth inhibitory effect of Helicobacter pylori

[0105] Cultivation of Helicobacter pylori strain

[0106]Helicobacter pylori strain ATCC 43504 (American Type Culture Collection, cagA+ and vacA s1-m1 strains) was incubated with 5% sheep blood on BBL's Tryptic Soy (TS) Agar Medium (TSAII; BD Biosciences, NJ Franklin Lakes, State) under microaerophilic conditions (BD GasPaK EZ gas generation system, BD Biosciences) at 37°C for 3 days. Helicobacter pylori strains were collected in TS medium, centrifuged at 3000×g for 5 minutes, and finally collected at a concentration of 10 9 Colony forming units (CFU) / ml were resuspended in medium. All experiments used cultures grown on TS agar medium for 72 hours.

[0107] Confirmation of Helicobacter pylori inhibitory ability

[0108] In order to confirm the inhibitory effect of the Helicobacter pylori of the Kimchi of the present application, in the medium inoculated with the Helicobacter pylori...

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Abstract

The present application relates to Kimchi containing lactic acid bacteria which can be effectively used in prevention and treatment of Helicobacter pylori-associated diseases.

Description

technical field [0001] The present application relates to kimchi containing lactic acid bacteria that can be effectively used for the prevention and treatment of Helicobacter pylori (Helicobacter pylori) related diseases. Background technique [0002] In 1983, Australian microbiologists Marchall and Warren first discovered Campylobacter in the gastric mucosal biopsy tissue of type B gastritis patients, then cultured the bacteria and identified it as a new species related to Campylobactergenus. Since then, the correlation between Helicobacter pylori and upper gastrointestinal diseases such as gastritis and peptic ulcer has been actively studied. Helicobacter pylori is a Gram-negative bacillus known to have flagella surrounded by a membrane, releases large amounts of urease, is transmitted via the fecal-oral route, and was identified as a carcinogen by the World Health Organization (WHO) in 1994 , thus becoming a pathogen of concern worldwide. [0003] Currently, the pathoge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L19/20A23L33/105A23L33/135A23L33/00A61K35/747A61K35/744A61P31/04A61P1/04
CPCA23L19/20A23L33/105A23L33/135A23L33/00A61K35/747A61K35/744A61P31/04A61P1/04A23V2002/00A23V2400/161A23V2400/169A23V2200/32A23V2200/3204A23V2250/21A61K2300/00A23B7/10A61K36/31Y02A50/30A23B7/105
Inventor 李东润崔承惠吴智暎
Owner CJ CHEILJEDANG CORP
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