A SNP Molecular Marker Influencing Net Gross Rate of Alpine Merino Sheep and Its Application

Abstract

The invention belongs to the technical field of molecular genetics, and in particular relates to a SNP molecular marker affecting the net wool rate of Alpine Merino sheep and its application. The SNP molecular marker is located at the T / C mutation at the 7370557th nucleotide site on chromosome 4 of the International Sheep Reference Genome Oar_v4.0 version. The present invention also relates to a specific primer pair for detecting the above-mentioned SNP molecular marker using PCR technology, a kit containing the primer pair and a detection method for nucleotide polymorphism. Compared with the traditional detection method, the technology has high accuracy and is It has the advantages of fast speed, low cost, and easy judgment of results. SNP locus detection is used to carry out early selection of alpine merino sheep wool rate traits, shorten the breeding cycle, speed up the breeding process, establish an early selection technology for alpine merino sheep wool rate traits, and reduce the number of alpine merino sheep. The breeding time of the excellent traits of net gross rate and the reduction of breeding cost have high application value.

Description

technical field [0001] The invention belongs to the technical field of molecular genetics, and in particular relates to a SNP molecular marker affecting the net wool rate of Alpine Merino sheep and an application thereof. Background technique [0002] Wool refers to a layer of fibers with textile value covering the surface of sheep, which is a derivative of skin. Wool is the main raw material of the wool spinning industry, accounting for about 97% of wool spinning raw materials, and is mainly used to process clothing fabrics, wool, blankets and other products. In the practice of wool production and breeding, wool traits are important economic traits, mainly including multiple indicators such as wool yield, average fiber diameter, curvature, breaking strength, elongation, and net wool rate. Among them, the net wool rate refers to the percentage of the weight of the pure wool fiber under the condition of the public moisture regain to the weight of the original wool sample, wh...

AI Technical Summary

Problems solved by technology

In recent years, fine-wool sheep genetics and breeding experts at home and abroad have been devoting themselves to the research of candidate genes or molecular markers controlling wool traits, but no molecular marker system for fine-wool traits has been established so far, and a large number of important genetic resources for regulating wool traits have not yet been established. be effectively mined and utilized

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