Construction method of liver cancer early screening model, detection device and computer readable medium

Abstract

The invention relates to a construction method of a liver cancer early screening model, a detection device and a computer readable medium. The lengths of WGS cfDNA read segments of 170 contrast groups and 192 liver cancer patients are counted, and the number of total fragments (40-300 bp), the number of short fragments (40-80 bp) and the number of super-long fragments (200-300 bp) are different between the number of total fragments (40-300 bp) and the number of short fragments (40-80 bp). Meanwhile, the number of fragments with different lengths is counted through long and short arms of chromosomes, and the two groups also have significant differences. According to the invention, a diagnosis model of the relationship between DNA fragment size sheet distribution and terminal sequence proportion and liver cancer is provided for the first time based on plasma cfDNA high-throughput low-depth sequencing, and the model not only can diagnose early liver cancer, but also can distinguish liver cirrhosis, and has the advantages of noninvasive detection, low throughput, and high detection specificity and sensitivity.

Description

technical field [0001] The invention relates to early screening of liver cancer (Hepatocellular Carcinoma, HCC), belonging to the technical field of molecular biomedicine. Background technique [0002] Liver cancer refers to malignant tumors that occur in the liver. There are about 600,000 new liver cancer patients in the world every year. The incidence rate ranks fifth in the world and the death rate ranks second. The onset of liver cancer is insidious, the transition process from hepatitis to cancer is long, and there are no obvious symptoms and signs in the early stage. Most patients are diagnosed in the middle and late stages when they are diagnosed, and the early diagnosis rate is low. The survival period of patients with advanced liver cancer in my country is less than 2 years. However, through early intervention, the five-year survival rate of liver cancer can reach 90%. [0003] There is still a lack of effective screening methods for liver cancer, and the detection...

AI Technical Summary

Problems solved by technology

The screening method of AFP combined with ultrasound has high requirements for patient compliance, and its accessibility is far from meeting the clinical requirements. At the same time, the diagnostic sensitivity of early liver cancer is insufficient, which restricts the effective implementation of current clinical screening methods; imaging detection still exists. There are certain limitations, and it cannot meet the needs of screening. Therefore, my country urgently needs to develop an effective, economical and practical screening method suitable for a wide range of people.

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