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Arsenic fluorescent quantum dot, and preparation method and application thereof

A technology of fluorescent quantum dots and molecular formulas, applied in the field of nanomaterials and nanobiology, can solve the problems of separation of drugs and fluorescent substances, loss of tracer function, etc., achieve maximum curative effect, simple and controllable preparation process, and reduce toxic and side effects

Active Publication Date: 2021-09-24
湖南中医药高等专科学校
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the nano-drug delivery system that combines drugs with fluorescent substances also has defects. Drugs and fluorescent substances may be separated in the body, losing the tracking function

Method used

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  • Arsenic fluorescent quantum dot, and preparation method and application thereof
  • Arsenic fluorescent quantum dot, and preparation method and application thereof
  • Arsenic fluorescent quantum dot, and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Preparation of arsenic fluorescent quantum dots:

[0038] Dissolve realgar in ethylenediamine, sonicate for 15 minutes, centrifuge at 15,000 rpm for 5 minutes, and take the supernatant. Disperse 1.2mL supernatant in 8mL water, stir for 5min, add 2.5mL HA aqueous solution (40mg / mL), stir for 5min, add 4.8mL 4M HCl, stir for 10min, then put the mixed solution in a 250mL three-necked flask, 75°C oil Bathed for 72h, the crude product was obtained. Finally, the centrifuged supernatant was collected, and the purified arsenic fluorescent quantum dots were obtained by separation through Sephadex gel column chromatography.

Embodiment 2

[0040] The fluorescence intensity of the arsenic fluorescent quantum dots formed in different oil bath times in Example 1 was investigated.

[0041] In the preparation method of Example 1, the 75°C oil bath was gradually extended from 12h to 72h, by figure 2 It can be seen that with the prolongation of the oil bath time, the fluorescence intensity gradually increased until reaching equilibrium at 72h.

Embodiment 3

[0043] The fluorescence intensity of arsenic fluorescent quantum dots under different excitation wavelengths was investigated.

[0044] Take As 4 S 4 @HA QDs scans the fluorescence emission spectrum at different excitation wavelengths to obtain the fluorescence spectrum, which is obtained by image 3 It can be seen that As 4 S 4 @HA QDs have strong fluorescence at the excitation wavelength of 350nm-410nm.

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Abstract

The invention provides an arsenic fluorescent quantum dot, a preparation method and application thereof. The molecular formula of the quantum dot is As4S4@HA QDs, wherein As4S4 and HA are realgar and hyaluronic acid respectively; hyaluronic acid serves as a stabilizer of the arsenic fluorescent quantum dot; and a stable HA hydration film is formed on the surface of the arsenic fluorescent quantum dot. The preparation method comprises the following steps of: dissolving the realgar in ethylenediamine, taking the hyaluronic acid as a stabilizer, and adding hydrochloric acid to adjust the pH value, so as to form the arsenic fluorescent quantum dot with a stable morphological structure. The quantum dot disclosed by the invention is simple in preparation method and stable in structure; intracellular delivery can be traced in real time in a spontaneous strong fluorescence mode; tumour cells are specifically recognized through the hyaluronic acid ligand; tumour targeted delivery is achieved; the tumour cells are selectively killed; potential toxic and side effects are reduced; and the quantum dot has wide application prospect.

Description

technical field [0001] The invention relates to the fields of nanomaterials and nanobiotechnology, in particular to an arsenic fluorescent quantum dot and its preparation method and application. Background technique [0002] Realgar (As4S4) is a representative traditional Chinese medicine arsenic compound, which has a broad anti-cancer spectrum. Its tumor therapeutic mechanism mainly includes: inducing tumor cell apoptosis, inhibiting tumor proliferation, promoting tumor cell differentiation and anti-tumor angiogenesis. However, in the anti-tumor application of realgar, there are many problems to be solved urgently. Realgar is a crystal with high lattice energy, which is difficult to dissolve in water and other common reagents. Its formulation has limitations, and it is often administered in large doses through oral route, but the absorption in the gastrointestinal tract is poor, and the bioavailability is low, which reduces the therapeutic effect. Effect, long-term use has...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K47/61A61K33/36A61P35/00B82Y5/00B82Y40/00
CPCA61K49/0019A61K49/0054A61K47/61A61K33/36A61P35/00B82Y5/00B82Y40/00
Inventor 李辉罗红梅周文虎陈双华唐婷
Owner 湖南中医药高等专科学校
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