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Method for confirming fever abatement target spot of common goldenrop particles

A technology of Xiaochaihu granules and targets, applied in the field of biomedicine, can solve the problems of unclear antipyretic mechanism, unclear mechanism of drug effect, and failure to confirm the antipyretic effect of Xiaochaihu granules.

Pending Publication Date: 2021-09-24
GUANGZHOU BAIYUSN GUANGHUA PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Modern pharmacological and clinical studies have shown that the formula of Xiaochaihu has antipyretic, anti-inflammatory, immune-regulating, anti-hepatic fibrosis, anti-allergic and other effects. The mechanism of the drug effect is not yet clear, especially the antipyretic mechanism or its target is still unclear
The applicants of the present invention previously published articles "Analysis of the Biological Target Network and Potential Mechanism of Xiaochaihu Granules" and "Analysis of the Compatibility Rules of Xiaochaihu Granules Based on Network Pharmacology" to explore the potential of Xiaochaihu Granules through network pharmacology technology. target, but the study still failed to confirm the antipyretic target of Xiaochaihu Granules

Method used

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  • Method for confirming fever abatement target spot of common goldenrop particles
  • Method for confirming fever abatement target spot of common goldenrop particles
  • Method for confirming fever abatement target spot of common goldenrop particles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1: chemical composition analysis

[0058] 1.1 Experimental method

[0059] 1.1.1 Experimental materials

[0060] Instruments: Shimadzu LC-30A, quadrupole-time-of-flight mass spectrometer (Triple Q-TOF5600+) from AB SCIEX, USA, pure water meter (Millipore), animal electronic scale (G&G), ultra-low temperature refrigerator (Thermo Scientific), electronic analysis Balance (Sartorius), vortex mixer (Qilin Bell), electronic thermometer (Omron), microplate reader (ThermoFisher).

[0061] Reagents and reagents: Xiaochaihu granules (Guangzhou Baiyunshan Guanghua Pharmaceutical Co., Ltd.), aspirin enteric-coated tablets (Bayer Healthcare Co., Ltd.), heparin sodium (Sigma-aldrich), chloral hydrate (Aladdin), PBS (pH7 .4) (Hyclone), dry yeast (Angel), PTGS2 kit (Biomatik), AP-1 kit (BioSource), MMP9, IL-1β, IFN-γ, IL-6, TNF-α, IL- 10. NF-κB kit (R&D).

[0062] Experimental animals: 48 10-week-old male, SPF grade SD rats, provided by Qinglongshan Animal Breeding Cent...

Embodiment 2

[0077] Example 2: Prediction of key targets based on network pharmacology

[0078] 2.1 Experimental method

[0079] 2.1.1 Acquisition and classification of targets of Xiaobupleurum granules

[0080] Use the PubChem database (https: / / pubchem.ncbi.nlm.nih.gov / ) to obtain the structure of the chemical components of Xiaobupleurum granules, and predict the chemical composition of each compound through the Swiss database (http: / / www.swisstargetprediction.ch / index.php). Targets, and the chEMBL database (https: / / www.ebi.ac.uk / chembl / ) was used to classify the targets.

[0081] 2.1.2 Acquisition of targets for febrile diseases

[0082] Retrieve targets related to human fever (Fever) through GeneCard database (https: / / www.genecards.org / ) and DisGeNET database (https: / / www.disgenet.org / ), and select Relevance score ≥ 5.9 ( 10 times the median of the target relevance score in the database (0.59) and the target with a score ≥ 0.3 (limited threshold) in the DisGeNET database are used as ...

Embodiment 3

[0097] Example 3: Target verification based on dry yeast pyrogenic rat model

[0098] According to the 68 key antipyretic targets of Xiaochaihu granules predicted by network pharmacology, 9 key antipyretic targets of Xiaochaihu granules were screened out: NF-κB, AP-1, TNF-α, IL-6, IFN-γ , IL-1β, PTGS2, IL-10, MMP9, combined with yeast-induced fever rat model to verify it.

[0099] 3.1 Experimental method

[0100] 48 rats were randomly divided into 6 groups, which were blank group, model group, positive drug aspirin group (0.081g / kg), low-dose Xiaochaihu granule group (5.4g / kg, clinical dose), middle-dose group (10.80 g / kg), high dose group (21.60g / kg). The rats grew adaptively for 7 days. The Xiaochaihu granule group was intragastrically administered once a day for 7 consecutive days, and the aspirin group was intragastrically administered once on the seventh day. The rectal temperature of the rats was measured twice within 1 hour before the last administration, and the av...

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Abstract

The invention discloses a method for confirming a fever abatement target spot of common goldenrop particles. The method comprises the following steps of screening chemical components and potential action targets of common goldenrop particles, and carrying out functional classification on the potential action targets by utilizing a chEMBL database; acquiring fever targets through a disease database, taking an intersection of potential action targets of common goldenrop particles and fever symptom targets, and constructing a common goldenrop particle fever abatement target database; performing functional analysis on the target spots, and screening the key target spots of the common goldenrop particles for abatement of fever; in combination with a dry yeast fever rat test, the cooling drug effect of the common goldenrop particles being defined, and the influence of the common goldenrop particles on the expression level of key targets NF-kappa B, AP-1, TNF-alpha, IL-6, IFN-gamma, IL-1beta, PTGS2, IL-10 and MMP9 being verified. The method finds that the common goldenrop granules are good in antipyretic curative effect and can maintain the body temperature for 24 hours without rebounding, and confirms that the antipyretic mechanism of the common goldenrop granules is associated with a plurality of links of body fever, including reduction of the inflammation level of the body, inhibition of endogenous pyrogen and synthesis of a main rate-limiting enzyme PTGS2 from a heating medium PGE2.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for confirming the antipyretic target of Xiaobupleurum granules. Background technique [0002] Xiaochaihu granule, a large variety of famous Chinese medicine, originated from the ancient famous prescription "Xiaochaihu Decoction" in "Treatise on Febrile Diseases" by Zhang Zhongjing, a medical sage in the Eastern Han Dynasty. Composed of traditional Chinese medicine, it has the effects of relieving the exterior and dissipating heat, soothing the liver and harmonizing the stomach. Modern pharmacological and clinical studies have shown that the formula of Xiaochaihu has antipyretic, anti-inflammatory, immune-regulating, anti-hepatic fibrosis, anti-allergic and other effects. The mechanism of drug efficacy is still unclear, especially the antipyretic mechanism or its target is still unclear. The applicants of the present invention previously published articles "Analysis of the B...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/72G01N30/8696G01N2030/324
Inventor 刘宏郑如文毕聪蒋研风羊江志强张俊华杜海泳
Owner GUANGZHOU BAIYUSN GUANGHUA PHARMA
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