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Rhodococcus gordoniae YKSW-6 and application thereof

A YKSW-6, Rhodococcus gordii technology, applied in the direction of bacteria, biochemical equipment and methods, microorganisms, etc., can solve the problems of interfering with the activity of skatole-producing bacteria, rising feed costs, environmental pollution, etc., and achieve efficient and rapid degradation capabilities , deodorizing effect is remarkable, the effect of good application prospect

Active Publication Date: 2021-09-28
HENAN ACAD OF SCI INST OF BIOLOGY LIABILITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it is possible to reduce the content of skatole in livestock and poultry feces by adding probiotics to affect the microbial flora structure in the intestinal tract of livestock and poultry and interfere with the activity of skatole-producing bacteria. However, there are still some problems in this method: first, the microecology Excessive addition of feed additives will lead to an increase in feed costs, which may lead to the absorption and digestion of feed nutrients; second, the residual skatole in the livestock and poultry manure that has been discharged into the environment still has great pollution to the environment. How to degrade livestock and poultry manure Skatole in composting and polluting the environment is still a problem we need to address
Burkeholderia (Burkehoderia), Achromobacter (Achromobacter) and Rhodococcus (Rhodococcus) are degrading bacteria of skatole in activated sludge, among which Rhodococcus strains DMU1 and DMU2 can completely degrade 50mg / L within 24h skatole, but whether this strain has a degradative effect on higher concentrations of skatole has not been reported

Method used

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  • Rhodococcus gordoniae YKSW-6 and application thereof
  • Rhodococcus gordoniae YKSW-6 and application thereof
  • Rhodococcus gordoniae YKSW-6 and application thereof

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Embodiment 1

[0022] When the present invention is concretely implemented, the preparation method of deodorizing Rhodococcus YKSW-6 bacterial agent comprises the following steps:

[0023] 1) Shake flask seed production: inoculate the YKSW-6 strain in the seed medium, and cultivate it to the logarithmic growth phase at 30°C and 180r / min;

[0024] 2) Fermentation culture: inoculate the seed solution obtained in step 1) into a large amount of culture fermentation medium according to the inoculation amount of 5%, the cultivation temperature is 30°C, the rotation speed is 180r / min, the initial pH is 7.2, and the cultivation time is 24h. After the fermentation is completed, the Microbial deodorant.

[0025] The deodorizing Rhodococcus YKSW-6 of the present invention is obtained by separating and screening from pig manure composting soil of a pig breeding factory in Zhongmou, Henan Province. It has high-efficiency and rapid degradation ability for high-concentration skatole, and can effectively de...

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Abstract

The invention relates to Rhodococcus gordoniae YKSW-6 and application thereof, can effectively solve the problem of degradation of high-concentration skatole, and adopts the technical scheme that the classification name of the Rhodococcus gordoniae YKSW-6 is Rhodococcus gordoniae, the Rhodococcus gordoniae YKSW-6 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No. 22972, and the preservation number is CGMCC No. 22972. The Rhodococcus gordoniae YKSW-6 is preserved in Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 yard, West Beichen Road, Chaoyang District, Beijing, on July 28, 2021, has efficient and rapid degradation capability on high-concentration skatole, has a degradation rate of 93.6% on skatole in compost, has a remarkable deodorization effect, is an innovation on skatole degradation microorganisms, and has broad application prospects.Good application prospects are realized.

Description

technical field [0001] The invention relates to the technical field of environmental protection, in particular to a strain of Rhodococcus gordii YKSW-6 and its application. Background technique [0002] With the rapid development of large-scale and intensified livestock and poultry breeding, livestock and poultry production can meet the needs of residents for meat, eggs, and milk, and the environmental pollution caused by a large amount of excrement and stench is gradually becoming the focus of attention. . Accompanied by a large amount of excrement, the emission and discharge of odorous substances have adverse effects on livestock production, residents' health, and air quality. [0003] Odor substances emitted by livestock and poultry have complex components and various types. According to their chemical properties, odor substances emitted by livestock and poultry can be divided into four categories, namely volatile nitrogen-containing compounds, volatile sulfur-containing...

Claims

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Application Information

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IPC IPC(8): C12N1/20B01D53/84C12R1/01
CPCC12N1/20B01D53/84B01D2251/95Y02A50/20
Inventor 张宗源张英涛岳丹丹周留柱郭文阳潘梦诗亓兰达王雪妍李冠杰杨书豪徐宏光王秋菊
Owner HENAN ACAD OF SCI INST OF BIOLOGY LIABILITY
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