Method for biological control of strawberry postharvest soft rot and strawberry fruit storage and preservation by debaryomyces hansenii Y3

A technology for storage and preservation and soft rot, which is applied to the biological control of postharvest soft rot of strawberry fruit in Debaria hansenii Y3. Unstable effects and other issues, to achieve significant social and ecological benefits, good growth, and avoid harm to the human body and the environment

Active Publication Date: 2021-10-01
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, at present, most antagonistic yeasts have shortcomings such as narrow bacteriostatic spectrum and unstable application effe...

Method used

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  • Method for biological control of strawberry postharvest soft rot and strawberry fruit storage and preservation by debaryomyces hansenii Y3
  • Method for biological control of strawberry postharvest soft rot and strawberry fruit storage and preservation by debaryomyces hansenii Y3
  • Method for biological control of strawberry postharvest soft rot and strawberry fruit storage and preservation by debaryomyces hansenii Y3

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Experimental program
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Effect test

Embodiment 1

[0028] The yeast strain provided by the present invention is isolated from the surface of strawberry fruit picked in Jurong Orchard, Zhenjiang; the specific Debaryomyces hansenii strain (Debaryomyces hansenii) Y3 and the pathogenic bacteria of soft rot Rhizopus stolonifer (R. stolonifer) The steps to obtain the method are as follows:

[0029] 1. A single colony of suspected yeast was isolated and purified from strawberry fruit, which was designated as yeast Y3;

[0030] Randomly sampled and weighed 20g of strawberry fruit, added to 150mL sterile water to shake and elute, and carried out gradient dilution to obtain 10 -1 、10 -2 、10 -3 、10 -4 solution. Draw 100 μL from the diluted solution and spread it on Bengal red medium, culture at 28°C for 24 hours, pick a single colony of dominant yeast, and isolate and purify it on NYDA medium. The isolated and purified strains were inoculated into NYDB medium at 28°C, cultured at 180r / min for 20-24h, and stored in a -80°C refrigerat...

Embodiment 2

[0048] Microbiological characteristics of Debaryomyces hansenii Y3 and Rhizopus stolonifer;

[0049] 1. Molecular biological identification

[0050] (1) Molecular biological identification of yeast Y3

[0051] For the isolated and screened yeast Y3, the nucleic acid sequence of the ITS region was amplified with fungal universal primers, and the sequencing results of the products were entered into the https: / / www.ncbi.nlm.nih.gov / website, and the homologous sequences were downloaded from the NCBI database. MEGA6 software constructs phylogenetic evolutionary tree such as image 3 , the yeast Y3 determined to be screened is Debaria hansenii; the yeast Y3 provided by the present invention for the prevention and treatment of strawberry fruit postharvest disease is now preserved in the Chinese Type Culture Collection Center (CCTCC), Wuhan University, Wuhan, China, and preserved The number is: CCTCC NO:M 2021610, the date of deposit is May 26, 2021, and the proposed classification...

Embodiment 3

[0058] Safety study of Debaryomyces hansenii strain Y3;

[0059] 1. Experimental protocol

[0060] The test animals were ICR mice with clean SPF grade, which were purchased from Changzhou Cavens Experimental Animal Co., Ltd., and a total of 40 mice with a weight range of 18g-22g were selected, with half male and half male. Before the experiment, the mice were quarantined in the Experimental Animal Center of Jiangsu University for 3 days. The test mice were divided into 4 groups, 10 in each group, 5 males and 5 males. The four groups of mice were divided into four dose groups of 0g / kg, 10g / kg, 21.5g / kg and 46.4g / kg for the experiment. The experiment was carried out by oral gavage at 0.4mL / 20g body weight. After 14 days of observation, during the whole experiment, observe whether there is any poisoning death in the daily condition of the mice. If mice appear, the test can be extended to 28 days if necessary. Calculate the median lethal dose and perform toxicity classificati...

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Abstract

The invention belongs to the technical field of fruit and vegetable postharvest biological control, and relates to a method for biological control of strawberry postharvest soft rot and strawberry fruit storage and preservation by debaryomyces hansenii Y3. The debaryomyces hansenii Y3 has the preservation number of CCTCC NO: M 2021610. The method comprises the following steps of activating, culturing and centrifuging yeast to obtain thalli, preparing 1*10<8> cells/mL yeast suspension by using sterile normal saline, soaking strawberry fruits in the yeast suspension, and naturally airing to realize biological control of strawberry postharvest soft rot and strawberry fruit storage and preservation. The method is safe and environment-friendly, can obviously inhibit the occurrence of the soft rot of the strawberry postharvest soft rot, and reduces the natural rotting rate of the harvested strawberry fruits; meanwhile, the main quality indexes of the harvested strawberry fruits are not affected, and the quality decline of the strawberry fruits can be delayed.

Description

technical field [0001] The invention belongs to the field of biological control of postharvest fruit diseases, and in particular relates to the biological control of postharvest soft rot of strawberry fruit by Debaria hansenii Y3 and a method for storing and keeping strawberry fruit fresh. Background technique [0002] Strawberry (Fragaria ananassa Duchesne) is a perennial herb belonging to the family Rosaceae and the genus Fragaria. Its fruit is delicious, red, tender and juicy, and contains a special rich fruit aroma, including essential vitamins, minerals, and trace elements. etc., as well as bioactive substances such as flavonoids and phenolic acids, have the reputation of "Queen of Fruits" and are one of the economical fruits loved by consumers. [0003] my country is one of the origins of strawberries. Nowadays, with the improvement of people's material life, people's dietary needs pay more attention to nutritional composition and flavor. Strawberry is a comprehensive ...

Claims

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Application Information

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IPC IPC(8): C12N1/16A01N63/32A01P3/00A23B7/155C12R1/645
CPCC12N1/16A01N63/32A23B7/155A23V2002/00A23V2200/10A23V2250/76
Inventor 赵利娜兰晨张红印张晓云王远见韩俊佳周游
Owner JIANGSU UNIV
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