Zein-polyphenol covalent complex and preparation method thereof

A technology of zein and complex, applied in vegetable protein processing, food science, application and other directions, can solve the problem of dark color of products, and achieve the effect of enhancing antioxidant properties, improving antioxidant properties, and improving antioxidant properties.

Pending Publication Date: 2021-10-08
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the non-covalent interaction between zein and polyphenols has been relatively thorough, but there are few studies on the covalent interaction between zein and polyphenols,

Method used

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  • Zein-polyphenol covalent complex and preparation method thereof
  • Zein-polyphenol covalent complex and preparation method thereof
  • Zein-polyphenol covalent complex and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0060] Example 1:

[0061] 1) 200 mg of corn alcohol is dissolved in 10 ml of 70% (v / v) ethanol-aqueous solution, ultrasonic 5 min, stirred for 30 min to completely dissolve, and add 0.001% (w / v) sorbic acid Potassium.

[0062] 2) 0.25 mm of chlorogenic acid (CGA) is dissolved in 10 mL 70% (v / v) ethanol-aqueous solution, ultrasonic 5min, stirred for 30 min to completely dissolve;

[0063] 3) At 4 ° C, 0.75 mm EDC was added to the polyphenol solution of step 2), followed by 0.5 mm NHS, stirring reaction 1 h;

[0064] 4) Polyphenol solution of step 3) is added to the corn alcohol solution of step 1), and the pH is adjusted to 6.5, and the reaction is reacted 1 h, and the reaction is 24 h at room temperature.

[0065] 5) After the reaction is completed, the mixture over the ultrafiltration unit, and the liquid obtained by ultrafiltration is placed in ultrasound.

[0066] 6) Treatment of dialyzed samples with nano grinding machine, pre-free, freeze-dried to give corn alcohol-sol...

Example Embodiment

[0069] Example 2:

[0070] 1) 200 mg of corn alcohol is dissolved in 10 ml of 70% (v / v) ethanol-aqueous solution, ultrasonic 5 min, stirred for 30 min to completely dissolve, and add 0.001% (w / v) sorbic acid Potassium.

[0071] 2) 0.25 mm ionic acid (Ga) was dissolved in 10 ml 70% (v / v) ethanol-aqueous solution, ultrasonic 5min, stirred for 30 min to completely dissolve;

[0072] 3) At 4 ° C, 0.75 mm EDC was added to the polyphenol solution of step 2), followed by 0.5 mm NHS, stirring reaction 1 h;

[0073] 4) Polyphenol solution of step 3) is added to the corn alcohol solution of step 1), and the pH is adjusted to 6.5, and the reaction is reacted 1 h, and the reaction is 24 h at room temperature.

[0074] 5) After the reaction is completed, the mixture over the ultrafiltration unit, and the liquid obtained by ultrafiltration is placed in ultrasound.

[0075] 6) Treatment of dialyzed samples with nano grinding machine, pre-free, freeze-dried to give corn alcohol-soluble prot...

Example Embodiment

[0076] Example 3: Measurement of covalent composites prepared in Examples 1 and 2

[0077] First, electric swim

[0078] The corn-CGAs (Zein-CGA) covalent composites prepared in Examples 1 and 2 were determined to covalent composites and civic acid (Zein-Ga) covalent complexes.

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Abstract

The invention discloses a method for preparing a zein-polyphenol covalent complex, and belongs to the technical field of food science and food processing. According to the invention, zein and plant polyphenol are used as raw materials, and the zein-polyphenol covalent complex is constructed in an ethanol-water solution by a carbodiimide-mediated coupling method. The preparation method has the characteristics of simplicity, high efficiency and safety. Compared with control zein, the prepared zein-polyphenol covalent complex has better thermal stability and solubility, and is obviously improved in oxidation resistance. The product disclosed by the invention can be used as a functional food additive and can also be used for constructing a novel delivery system. In addition, the zein-polyphenol covalent complex prepared by the method still keeps the original faint yellow color of a protein, is more easy to accept by consumers, and is beneficial for expanding the application range of the zein-polyphenol covalent complex.

Description

technical field [0001] This patent specifically relates to a preparation method of a zein-polyphenol covalent compound, which belongs to the technical fields of food science and food processing. Background technique [0002] Zein is a natural plant protein isolated from corn seeds. The main commercially used α-zein protein has a molecular weight of about 22 kDa. Zein has good biodegradability and biocompatibility, and is often used to construct delivery systems to deliver biologically active substances. There are a large number of non-polar amino acids and α-helical structure in the zein molecule, which leads to its strong surface hydrophobicity and almost insoluble in water. In addition, natural zein has weak antioxidant properties. The low water solubility and antioxidant activity of zein may limit its application in food. In recent years, a large number of studies have shown that covalently grafting polyphenols on proteins can improve the solubility and antioxidant pro...

Claims

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Application Information

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IPC IPC(8): A23J3/14
CPCA23J3/14A23V2002/00A23V2250/5482A23V2250/2132Y02W90/10
Inventor 魏子淏徐雅男薛长湖
Owner OCEAN UNIV OF CHINA
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