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Application of total alkaloids of cynanchum komarovii in preparation of anti-inflammatory and analgesic drugs

A total alkaloid, anti-inflammatory and analgesic technology, applied in the field of biomedicine, can solve the problem that the analgesic effect of medicinal value has not been paid attention to

Pending Publication Date: 2021-10-08
NINGXIA MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The resource survey of Laoguatou shows that Laoguatou is rich in resources in Ningxia, especially in Yanchi, Zhongwei, Qingtongxia and other places. However, most of the local people treat it as a grassland poisonous grass, and its medicinal value is especially analgesic. role has not been taken into account
And in the prior art, there is no application of the total alkaloids of melon head in analgesia

Method used

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  • Application of total alkaloids of cynanchum komarovii in preparation of anti-inflammatory and analgesic drugs
  • Application of total alkaloids of cynanchum komarovii in preparation of anti-inflammatory and analgesic drugs
  • Application of total alkaloids of cynanchum komarovii in preparation of anti-inflammatory and analgesic drugs

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The preparation of embodiment 1 old melon head total alkaloids

[0046] see Figure 1A , dry the collected old melon head whole grass, cut into sections, pulverize, weigh, put the medicinal powder into gauze bags, put it into an ultrasonic extraction tank, add 75% ethanol at a material-to-liquid ratio of 1:10 for ultrasonic extraction , extract 2.5h each time, repeat 3 times, discard the dregs; the extract is concentrated by a rotary evaporator at 50°C under reduced pressure to obtain Laoguatou fluid extract, which is then dissolved with 2% hydrochloric acid, filtered with suction, and insoluble Extract with a 5L separatory funnel, first remove the fat-soluble components by petroleum ether extraction, combine the acidic water layers, add concentrated ammonia water dropwise to adjust the pH to 9-11, and then extract with dichloromethane until bismuth potassium iodide reacts to form Negative, the combined extracts were concentrated by a rotary evaporator at 50°C under red...

Embodiment 2

[0048] Embodiment 2: Mouse thermal sting test of total alkaloids of melon head

[0049] Animals: ICR mice (18-20g), half male and half male, purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., raised in the clean-grade barrier space of China Academy of Chinese Medical Sciences, room temperature 25±1°C, relative humidity 50-70% . They were irradiated by fluorescent lamp for 12 hours in a cycle, fed with conventional feed, free to eat and drink, watered, and adaptively fed for 3 days before being used for the experiment.

[0050] Grouping and administration: ICR mice were randomly divided into groups according to body weight, namely blank group, positive drug group (tramadol hydrochloride 40 mg / kg, THSRT), TACKI (400, 200, 100 mg / kg) group. The administration volume of 0.2mL / 10g was given by intragastric administration for 7 days continuously, and the blank group was given distilled water in the same way.

[0051] Determination of the latent period o...

Embodiment 3

[0053] Embodiment 3: Mouse formalin experiment of total alkaloids of melon head

[0054] The mice were randomly divided into 5 groups according to body weight, namely blank group (distilled water), positive drug group (Dolantin 20mg / kg, Dolantin), TACKI (400, 200, 100mg / kg) group, 10 in each group, old melon The total alkaloids of the head were administered by intragastric administration continuously for 7 days. 2 hours after the last administration, 30 minutes after the subcutaneous injection of peperidine, 20 μL of 2.5% formalin was subcutaneously injected into the sole of the mouse's right foot with a micro-injection needle. Observe and record the foot licking time of phase I (0-5mim) and phase II (15-30min).

[0055] Result: if image 3 As shown, compared with the Model group, TACKI significantly reduced the foot licking time of mice in phase I in a dose-dependent manner, and TACKI 400mg / kg and TACKI 200mg / kg significantly reduced the foot licking time of mice in phase II...

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Abstract

The invention discloses application of total alkaloids of cynanchum komarovii in preparation of anti-inflammatory and analgesic drugs. The research finds that the total alkaloids of cynanchum komarovii can prolong the thermal withdrawal latency period of a mouse in a thermal stabbing pain experiment, shorten the paw licking time of the mouse in a formalin pain experiment; and prolong the pain latency period of the mouse in an acetic acid writhing experiment, and reduce the writhing frequency, thereby effectively alleviating the pain response of the mouse. In addition, the total alkaloids of cynanchum komarovii can prolong the thermal withdrawal latency period and increase the mechanical withdrawal threshold of CIA rats, which indicates that the total alkaloids of cynanchum komarovii also have a mitigative effect on chronic inflammatory pain. Meanwhile, the total alkaloids of cynanchum komarovii can remarkably inhibit expression of inflammatory factors such as GFAP, NMDAR2B, TNF-a, IL-6 and IL-17, so that the total alkaloids of cynanchum komarovii can be used as a main component of anti-inflammatory and analgesic drugs, thus providing a new way for research and development of new drugs.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to the application of total alkaloids of melon head in the preparation of anti-inflammatory and analgesic drugs. Background technique [0002] The International Association for the Study of Pain (IASP) has revised the definition of pain: Pain is a discernible bodily experience that reflects a person's fear of a threat to his body or bodily integrity. Many clinical diseases are accompanied by pain symptoms, and the degree of pain is closely related to the degree of pain of the patient, which not only seriously affects the quality of life and work efficiency of the patient, but also causes a serious economic burden. It is reported that the annual cost of treating chronic pain in the United States It has reached 635 billion US dollars. Therefore, effective pain relief has become one of the important topics in clinical treatment and analgesic drug research. [0003] At...

Claims

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Application Information

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IPC IPC(8): A61K36/27A61P29/00
CPCA61K36/27A61P29/00A61K2236/333A61K2236/55
Inventor 白长财王瑞洲李冰歌韩璐高晓娟陶丽郝福生范红丽
Owner NINGXIA MEDICAL UNIV
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