Enzyme conjugate diluent, AMH quantitative detection kit and use method thereof

Abstract

The invention provides an enzyme conjugate diluent, an AMH quantitative detection kit and a use method thereof, and the enzyme conjugate diluent comprises EDTA, casein, a buffer solution and protective protein. The AMH quantitative detection kit comprises a coated plate coated with an anti-AMH monoclonal antibody, an enzyme conjugate containing an enzyme-labeled anti-AMH monoclonal antibody and an AMH calibrator, and an enzyme conjugate diluent and the enzyme-labeled anti-AMH monoclonal antibody are mixed according to the volume ratio of (7800-8300):1 to obtain the enzyme conjugate. In the detection process, the active ingredients of the difference between new and old samples are eliminated, the difference between the new and old samples caused by complement effect is reduced, in addition, the kit adopts a one-step reaction, the reaction time is shortened, the operation steps are simplified, a more convenient and faster operation method is realized, and the kit is more flexible and convenient to operate.

Description

technical field [0001] The invention belongs to the technical field of immunodiagnosis, and in particular relates to an enzyme conjugate diluent, an AMH quantitative detection kit and a use method thereof. Background technique [0002] Anti-Müllerian hormone (AMH) is one of the members of the transforming growth factor β superfamily, first discovered by Professor AlfredJost. AMH is a dimeric glycoprotein composed of two identical 70KDa subunits linked by disulfide bonds, with a relative molecular mass of 140KDa[1]; the human AMH encoding gene is located on the short arm of chromosome 19, with a size of 2.4-2.8kb 5 exons. AMH plays an important role in the development of gonad organs and is one of the important markers of gonad function in men and women. Male AMH is mainly produced by Leydig cells of the testis, starting from embryogenesis and throughout life; during the development of male fetuses, AMH leads to the degeneration of Mullerian ducts and the formation of norma...

AI Technical Summary

Problems solved by technology

[0004] At present, kits prepared by enzyme-linked immunoassay and electrochemiluminescence are mainly used for clinical detection of AMH. The disadvantages of electrochemiluminescence are high detection costs, potential problems of cross-contamination, and relatively high environmental requirements. High, the current disadvantages of enzyme-linked immunosorbent assay are long detection time and complicated operation steps

At present, the operation process of ELISA at home and abroad: use solid-phase anti-AMH antibody, biotin-labeled anti-AMH antibody, alkaline phosphatase-labeled horseradish peroxidase to form biotin-alkaline phosphate The "sandwich" structure of the double-antibody sandwich method including enzymes, the operation steps are mostly three-step or two-step methods, the operation steps are cumbersome, and there are still differences between old and new samples in AMH detection

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