Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Close linkage marker for controlling related genes of soybean leaf color and application of close linkage marker

A soybean leaf and gene technology, applied in the field of breeding and molecular genetics, can solve problems such as low efficiency and restricting the development of soybean functional genomics

Inactive Publication Date: 2021-10-15
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, complex genome structure, low-efficiency genetic transformation system and many other factors limit the development of soybean functional genomics research.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Close linkage marker for controlling related genes of soybean leaf color and application of close linkage marker
  • Close linkage marker for controlling related genes of soybean leaf color and application of close linkage marker
  • Close linkage marker for controlling related genes of soybean leaf color and application of close linkage marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. For the phenotypic identification of wild-type and mutant materials, refer to "Description Specifications and Data Standards for Soybean Germplasm Resources" edited by Qiu Lijuan et al. From 2016 to 2018, at the Beijing Shunyi Experimental Station of the Institute of Crop Science, Chinese Academy of Agricultural Sciences, the leaf yellow mutant H745 and the wild-type soybean variety ZP661 were observed and identified for the entire growth period, especially the field phenotypes of the blooming and mature stages. Chlorophyll content identification was carried out at the full flowering stage, and 10 plants of ZP661 and H745 were selected for testing at the mature stage, and the main agronomic traits such as plant height, bottom pod height, number of nodes, number of branches, number of pods per plant, and 100-grain weight were measured and photographed. .

[0029] 2. Determination of photosynthetic pigment content

[0030] At the full flowering stage (R2), the wild ty...

Embodiment 2

[0040] 1. Genetic population construction

[0041] At the Beijing Shunyi Experimental Base of the Chinese Institute of Agricultural Sciences, ZP661 was used as the female parent and the yellow mutant H745 was used as the male parent to prepare a hybrid combination and obtain hybrid seeds. In the summer of 2017, the F1 generation plants were propagated and the seeds were harvested at the same location. In 2018, the F2 generation population was planted at the same location, and the F3 generation population was continued to be planted and phenotyped in 2019 to verify the genotype of the F2 population.

[0042] 2. Genomic DNA Extraction

[0043] Sample preparation: Weigh 0.1g to 0.5g of young soybean leaves and place them in a 2mL centrifuge tube, mark the sample number on the tube cover and tube wall, add steel balls, freeze in liquid nitrogen, and shake on a shaker until Grind the leaves into powder. Cleavage: Add 2% CTAB (1 / 1000 volume β-mercaptoethanol) extract at 600°C, sha...

Embodiment 3

[0065] Based on the sequencing data in the candidate interval, 9 SNP sites located on the gene were selected from the 19 SNP sites in the association interval that conformed to the rule of BSA pool construction ( Figure 8), respectively for SNP verification and CAPS / dCAPS development (according to the variant sites obtained in the resequencing data, and then obtain the SNP positions from the Phytozome website https: / / phytozome.jgi.doe.gov / pz / portal.html# Click on the relevant sequence in the attachment to design primers for identification). After identification, the SNP at H745-1 among the 9 sites was false, the amplified fragment of H745-4 was a homologous sequence, and the amplified band of H745-8 was double peaked; the other 6 sites were all mutant SNPs (i.e. The variation is consistent with the sequencing results), successfully developed as CAPS / dCAPS markers for identification of populations.

[0066] 481 F2:3 populations were detected by 6 CAPS / dCAPS markers, and the c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a close linkage marker for controlling related genes of soybean leaf color and application, and belongs to the technical field of breeding and molecular genetics. The nucleotide sequence is shown as SEQ ID NO. 1. And the molecular marker H745-5 closely linked with the related gene for controlling the soybean leaf color is located in the No.11 chromosome. According to the invention, a soybean yellowing mutant H745 obtained by screening ZP661 mutagenized by EMS is used as a material, phenotype identification is carried out through chlorophyll content determination and chloroplast ultrastructure observation, BSA-seq sequencing is carried out by using yellow and green structural pools in a ZP661x H745 (F2) segregation population, and linkage analysis is carried out by combining the segregation population to confirm an interval; a dCAPS marker H745-5 is developed aiming at a variation site of a candidate gene, a ZP661xH745 (F2) population is identified by utilizing the developed dCAPS marker to obtain genotype data, and the accuracy and reliability of the marker are identified.

Description

technical field [0001] The invention relates to the technical field of breeding and molecular genetics, and more specifically relates to a tightly linked marker and application of genes related to controlling soybean leaf color. Background technique [0002] Soybean is one of the most important economic crops in the world, providing human beings with rich vegetable protein and fat. The completion of soybean genome sequencing will promote the rapid development of soybean functional genomics. [0003] However, complex genome structure, low-efficiency genetic transformation system and many other factors limit the development of soybean functional genomics research. Mutants are important materials for important gene mining and functional research. Due to the low-efficiency genetic transformation system and genotype dependence, the application of T-DNA insertion and transposon mutagenesis technology based on the transgenic platform in the screening of soybean mutants has been h...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 邱丽娟高华伟洪慧龙刘章雄
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com