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Method for realizing accurate detection of beta-amyloid protein by using Fe3O4@MXene core-shell structure nanocomposite

A nanocomposite and amyloid protein technology, which is applied in the field of accurate detection of β-amyloid protein, can solve the problems of reduced accuracy of detection results, lower signal-to-noise ratio of fluorescence detection, etc., and achieve improved portability and good fluorescence quenching effect , The effect of high sensitivity detection

Pending Publication Date: 2021-10-15
FUDAN UNIV
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Problems solved by technology

However, due to the limitation of autofluorescence generated by pure MXene materials, the signal-to-noise ratio of fluorescence detection is reduced, and the accuracy of detection results is reduced.

Method used

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  • Method for realizing accurate detection of beta-amyloid protein by using Fe3O4@MXene core-shell structure nanocomposite
  • Method for realizing accurate detection of beta-amyloid protein by using Fe3O4@MXene core-shell structure nanocomposite
  • Method for realizing accurate detection of beta-amyloid protein by using Fe3O4@MXene core-shell structure nanocomposite

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[0030] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention. In addition, the technical features involved in the various embodiments of the present invention described below can be combined with each other as long as they do not constitute a conflict with each other.

[0031] figure 1 It is a flow chart of the method for realizing the accurate detection of β-amyloid protein in the present invention, which mainly includes Fe 3 o 4 @MXene synthesis, fluorescence quenching and recovery, and microfluidic chip detection are three parts, as follows:

[0032] Fe 3 o 4 Synthesis of @MXene: Fe 3 o 4 The outer layer of nanospheres first wraps PDDA to form PDDA-Fe...

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Abstract

The invention discloses a method for realizing accurate detection of beta-amyloid protein by using a Fe3O4@MXene core-shell structure nanocomposite. According to the method, an FAM modified beta-amyloid protein aptamer (FAM-aptA beta) is used as a fluorescence donor, and a Fe3O4@MXene core-shell structure nanocomposite is used as a fluorescence receptor. FAM-aptA beta fluorescence quenching is achieved by using the fluorescence quenching property of MXene, fluorescence recovery is achieved by using the strong affinity of A beta and FAM-aptA beta, different fluorescence recovery effects are achieved by A beta with different concentrations, and finally accurate detection of A beta is achieved by using a multi-volume micro-fluidic chip as a fluorescence detection device. The method overcomes the defects that a traditional detection method needs large equipment, the detection precision is low and the period is long, rapid and accurate detection of the disease markers is achieved, and the method has important reference value for instant inspection of clinical disease markers.

Description

technical field [0001] The invention relates to the technical fields of biosensing, functional materials and micro-nano detection, specifically a method using Fe 3 o 4 A method for accurate detection of β-amyloid protein by @MXene core-shell nanocomposite. Background technique [0002] Alzheimer's disease (AD) is the most common neurodegenerative disease clinically characterized by progressive dementia. To date, there is still no effective treatment, making the disease a very serious health and social problem. Although the pathogenesis of AD is still unclear, a large number of studies have clearly shown that abnormal aggregation of amyloid β-peptide (Aβ) in the brain is a typical feature of AD neuropathology. Aβ can not only form tangles of regularly organized fibrous aggregates in the brain but also form highly toxic soluble oligomers. Aβ oligomers are the main pathogenic factor in AD pathology. Therefore, sensitive detection of Aβ oligomers under physiological conditio...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/58C09K11/06C09K11/02C09K3/00
CPCG01N33/6896G01N33/582C09K11/06C09K11/025C09K3/00G01N2333/4709C09K2211/1088
Inventor 卢红亮温小红
Owner FUDAN UNIV
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