Blood flow infection pathogen multi-gene detection system, and kit and application thereof

Abstract

The invention relates to a blood flow infection pathogen multi-gene detection system, and a kit and application thereof. The detection system has 25 pairs of primers, including 24 pairs of detection primers and 1 pair of system quality control internal reference primers. According to the blood flow infection pathogen multi-gene detection system, and the kit and application thereof, conventional detection is not needed, synchronous detection and analysis of various pathogen genes can be directly carried out on blood subjected to enrichment culture in the same reaction system, and the defects that a conventional detection method is low in flux, long in consumed time and the like are overcome; and comprehensive, accurate and low-cost etiological diagnosis is provided for clinic for the first time, and important reference is provided for individualized medication and accurate medical treatment.

Description

technical field [0001] The invention relates to a multiple gene detection product and a detection system used in the product, belonging to the field of biotechnology. Background technique [0002] Bloodstream infection (bloodstream infection) is a very common critical illness among hospitalized patients. It has a high incidence rate. More than 1.8 million cases of severe bloodstream infection occur every year in the world. According to the multi-center epidemiological survey in my country, There are approximately 3.73 million cases of severe bloodstream infection in my country every year. Moreover, the bloodstream infection progresses rapidly, there are many critically ill patients, and the fatality rate is high. According to foreign epidemiological surveys, in intensive care units, the fatality rate of bloodstream infection has surpassed myocardial infarction and has become the main cause of death for non-cardiac patients. If septic shock is concurrent, the fatality rate of...

AI Technical Summary

Problems solved by technology

However, these PCR-based methods also encounter some problems when they are used to detect pathogens in bloodstream infection samples: first, the concentration of pathogens in the blood of patients with bloodstream infection is very low, usually <10CFU / mL, which is significantly lower than PCR The lower limit of detection of the method leads to a high false negative rate; secondly, if the blood sample is incubated and enriched first, and then PCR is used to prevent detection, although the concentration of pathogens can be increased, the PCR inhibitors in the blood culture bottle will also increase. Will interfere with PCR detection, still resulting in a very low positive rate

[0005] In summary, the current detection methods for bloodstream infection pathogens cannot meet the clinical needs, and there is an urgent need for a method that can enrich and enrich pathogens in blood samples and remove PCR inhibitors in blood and blood culture bottles, so that subsequent The molecular biological detection method can be carried out smoothly, providing timely and accurate etiological diagnosis basis for clinic

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