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Bacillus coagulans with functions of preventing diarrhea and degrading cholesterol

A technology of bacillus coagulans and cholesterol, which is applied to the functions of food ingredients, medical preparations containing active ingredients, bacteria, etc., and can solve the problems of uneven product quality, impact on the immune system, large differences in application effects and stability, etc. question

Active Publication Date: 2021-10-22
QINGDAO VLAND BIOTECH INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

3) Affect the immune system
[0005] At present, the application effect and stability of probiotic products on the market vary greatly, and the quality of the products is uneven, which cannot meet people's growing demand for probiotic products. Therefore, screening high-quality probiotic resources, especially Bacillus resources, remain the focus of research in this field

Method used

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  • Bacillus coagulans with functions of preventing diarrhea and degrading cholesterol
  • Bacillus coagulans with functions of preventing diarrhea and degrading cholesterol
  • Bacillus coagulans with functions of preventing diarrhea and degrading cholesterol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Isolation and screening of embodiment 1 bacterial strain

[0037] 1. Primary screening of Bacillus

[0038] Take 1g of fermented sour cabbage, smash it with a masher, soak it with 10ml of 0.85% physiological salt for 10 minutes, put it into a sterile sample bag, beat it with a homogenizer, and pour it into a test tube, set the water bath at 70°C Heated for 10 minutes, and the suspension was serially diluted, and 10 -1 、10 -2 、10 -3 Three dilution gradients of 100ul were spread on the MRS medium, and cultured in a 37°C incubator under aerobic conditions for 48h. After a single colony grew on the plate, microscopic examination was performed respectively. According to the results of microscopic examination, the applicant screened out 10 bacillus strains, which were named NH-1, NH-2, ..., NH-10 respectively.

[0039] 2. Re-screening of Bacillus

[0040] Liquid fermentation medium: weigh 3.0g yeast powder, 5.0 peptone, 2.0g beef extract, K 2 HPO 4 3g, MnSO 4 0.005g, M...

Embodiment 2

[0049] Example 2 strain identification

[0050] 2.1 Identification of colony morphology

[0051] The single colony of the NH-8 strain is milky white and shiny, the surface of the colony is smooth and moist, the edges are neat, and the diameter of the colony is 2-3mm. Under the microscope, it is rod-shaped, arranged in single, pair or chain, and the spores are terminal.

[0052] 2.2 Identification of physiological and biochemical characteristics

[0053] The preparation of the inoculum in this embodiment is as follows: under sterile conditions, take an appropriate amount of fresh bacterial solution, centrifuge at 5000rpm for 5min, wash 2 times with PBS buffer, then resuspend with the same volume of PBS buffer and dilute 50 times as the inoculum. liquid.

[0054] 1. Temperature growth range experiment

[0055] Under sterile conditions, the inoculum was inoculated into 10 mL of MRS liquid medium at a 10% inoculation amount, and 10 mL of MRS liquid medium without inoculation w...

Embodiment 3

[0113] Example 3 Bacillus coagulans VHProbi C08 Salinity Tolerance Test

[0114] Under sterile conditions, inoculate 10% of the inoculum into 5 mL of MRS liquid medium with salt concentrations of 1%, 2%, 3%, 4%, 5%, 6%, 7%, and 8%. In this method, 5mL of MRS liquid medium without inoculation was used as a control, and placed in a constant temperature shaking culture at 37°C to observe whether the medium became turbid.

[0115] The results showed that Bacillus coagulans VHProbi C08 grew at a salt concentration of 1% to 7%, and did not grow at a salt concentration of 8%. The maximum salt concentration of Bacillus coagulans VHProbi C08 was 7%.

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Abstract

The invention relates to the technical field of functional microorganism screening and application, and particularly provides a novel bacillus coagulans and application of thereof. The bacillus coagulans is screened from a sour cabbage sample, the preservation number is CCTCC NO: M2019738, and the bacillus coagulans has high oxidation resistance and cholesterol degradation capacity, can effectively regulate intestinal tract movement, prevent diarrhea, recover the microbial barrier function of intestinal tracts and shorten the disease course, and is very remarkable in effect.

Description

technical field [0001] The invention relates to the technical field of screening and application of probiotics, in particular to a strain of bacillus coagulans capable of preventing diarrhea and degrading cholesterol and its application. Background technique [0002] The word probiotics originated from Greek. With the deepening of people's understanding, keywords such as regulating intestinal flora, endogenous microorganisms, living microorganisms, and the number of viable bacteria have also been integrated into the concept of probiotics. Currently, the widely accepted definition is the definition of the Food and Agriculture Organization of the United Nations / World Health Organization, that is, active microorganisms that have beneficial effects on the host by improving the balance of intestinal flora. In a broad sense, all strains that can meet the basic conditions of probiotics can become probiotics. Therefore, probiotics cover many species of microorganisms, and currently...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/742A61K35/747A61K35/745A61K35/744A61K35/741A61K36/064A61P1/12A61P3/06A23L33/135A23L33/14C12R1/07
CPCA61K35/742A61K35/747A61K35/745A61K35/744A61K35/741A61K36/064A61P1/12A61P3/06A23L33/135A23L33/14A23V2002/00A61K2300/00A23V2200/3204A23V2200/3262
Inventor 段治崔洪昌张景燕郭超群张陆霞
Owner QINGDAO VLAND BIOTECH INC
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