Single-base extension primer group and method for detecting polymorphism of vitamin A metabolism related gene

Abstract

The invention relates to the technical field of gene detection, and particularly discloses a single-base extension primer group and a method for detecting polymorphism of a vitamin A metabolism related gene. The single-base extension primer group at least comprises one of primers of which the nucleotide sequences are as shown in SEQ ID NO.1-15. The detection method comprises the following steps: respectively designing a multiple PCR amplification primer group and the single base extension primer group, carrying out PCR amplification by taking the multiple PCR amplification primer group as primers, and then carrying out an enzyme digestion reaction; then adding the single-base extension primer group to carry out a single-base extension reaction; and finally, purifying the single-base extension product, and carrying out mass spectrometric detection to obtain the polymorphism of the vitamin A related gene of the sample to be detected. The detection method provided by the invention has the advantage of high-throughput detection of gene polymorphic sites.

Description

technical field [0001] This application relates to the technical field of gene detection, more specifically, it relates to a single base extension primer set and a method for detecting gene polymorphisms related to vitamin A metabolism. Background technique [0002] The low absorption and utilization of vitamins caused by genetic defects is an important reason for vitamin deficiency. The low absorption and utilization of vitamins can be compensated by a large intake of required vitamins. However, without knowing the low absorption and utilization capacity of vitamins, it is often impossible to supplement the required vitamins in a targeted manner; and then recurring symptoms of vitamin deficiency: such as slow eye adaptation in dimly lit environments, such as lips, skin, etc. Dryness, such as barbs on the hands; these adverse symptoms may be due to vitamin deficiency. Long-term vitamin deficiency can lead to irreversible damage or more serious disease. [0003] At present,...

AI Technical Summary

Problems solved by technology

In the detection process, the amplification primers selected by the existing detection methods have the problem of low specificity

Moreover, the existing detection method can only detect the genetic polymorphism type of one gene / locus at a time, which makes the detection method have the problems of long detection cycle and low throughput, which ultimately leads to low detection efficiency

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