36 results about "Vitamin metabolism" patented technology

Provided are methods of detecting the presence or amount of a vitamin D metabolite in a sample using mass spectrometry. The methods generally comprise ionizing a vitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. Also provided are methods to detect the presence or amount of two or more vitamin D metabolites in a single assay.

Provided are methods of detecting the presence or amount of a dihydroxyvitamin in D metabolite in a sample using mass spectrometry. The methods generally comprise ionizing a dihydroxyvitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. In certain preferred embodiments the methods include immunopurifying the dihydroxyvitamin D metabolites prior to mass spectrometry. Also provided are methods to detect the presence or amount of two or more dihydroxyvitamin D metabolites in a single assay.

Provided are methods of detecting the presence or amount of a dihydroxyvitamin in D metabolite in a sample using mass spectrometry. The methods generally comprise ionizing a dihydroxyvitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. In certain preferred embodiments the methods include immunopurifying the dihydroxyvitamin D metabolites prior to mass spectrometry. Also provided are methods to detect the presence or amount of two or more dihydroxyvitamin D metabolites in a single assay.

Provided are methods of detecting the presence or amount of a vitamin D metabolite in a sample using mass spectrometry. The methods generally comprise ionizing a vitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. Also provided are methods to detect the presence or amount of two or more vitamin D metabolites in a single assay.

A method for quantitating vitamin D metabolites directly in blood plasma or serum, without the need for prior purification of the vitamin D metabolites, comprising a digestion of the serum proteins with a serine protease such as proteinase K and sequence of steps for inhibiting the proteinase K activity in the competitive binding analysis. The advantages of this method are its high accuracy over the whole range of physiologically relevant values and that it can be easily adapted for a fully automated analysis of serum and plasma samples.

The invention relates to the detection of vitamin D metabolites. In a particular aspect, the invention relates to methods for detecting derivatized vitamin D metabolites by mass spectrometry.

A method is provided for identifying an individual as having altered vitamin D metabolism comprising analyzing a biological sample from the individual for the presence of CYP24 SNPs and / or aberrantly spliced CYP24 mRNA. The presence of the SNPs and / or the aberrantly spliced CYP24 mRNA indicates that the individual has altered vitamin D metabolism. Also provided are methods for customizing dosages of biologically active vitamin D compounds for individuals who are determined to have altered vitamin D metabolism.

The present invention provides a method of preventing or treating a disease caused by oxidation in vivo by administering a pharmacologically effective amount of at least one compound selected from the group consisting of: (1) 2,5,7.8-tetramethyl-2-(beta-carboxyethyl)-6-hydroxychromane; and (2) 2,7.8-trimethyl-2-(beta-carboxyethyl)-6-hydroxychromane. Further, it provides use of a compound selected from the group consisting of (3) a-tocopherol, (4) a-tocotrienol, (5) gamma-tocopherol and (6) gamma-tocotrienol for generation in vivo of any of the above compounds (1) and (2) to treat a disease caused by oxidated low density lipoprotein (LDL). As the above-mentioned disease, arteriosclerosis is particularly preferred.

The purpose of the present invention is to provide an antibody against fibroblast growth factor 23. The antibody is obtained by immunizing an animal with a polypeptide which comprises an amino acid sequence represented by SEQ ID NO: 1, or an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 1 by deletion, substitution, or addition of 1 or several amino acids, and has fibroblast growth factor-23 activity and activity to control phosphate metabolism or vitamin D metabolism, and is shown by the following (a), (b), or (c): (a) an antibody, which recognizes an amino acid sequence between the 180 and the 194, or the 237 and the 251<st> amino acid residues represented by SEQ ID NO: 1; (b) an antibody, which is produced by a hybridoma whose accession number is FERM BP-7838, FERM BP-7839, FERM BP-7840, or FERM BP-8268; or (c) an antibody, which is competitive with the antibody produced by the hybridoma whose accession number is FERM BP-7838, FERM BP-7839, FERM BP-7840, or FERM BP-8268 upon binding with the polypeptide comprising the amino acid sequence represented by SEQ ID NO: 1. <IMAGE>

The invention relates to a rapid sensitivity analysis method for 25-hydroxyvitamin D based on an immune mass spectrometry technology, which comprises the following steps: S1, enrichment and purification: performing sampling, adding an isotope internal standard solution and biotinylated antibody magnetic beads, carrying out oscillation incubation, and sequentially washing the sample with a magneticbead cleaning solution and pure water after incubation; s2, one-step derivative elution: adding the washed magnetic beads into an acetonitrile solution containing 4-phenyl 1, 2, 4-triazoline 3, diketone, carrying out oscillation incubation to carry out derivative elution, and adding an aqueous solution containing CH3NH2 into a derivative eluent to terminate the reaction; and S3, liquid chromatography-mass spectrometry detection: transferring the derivative eluent to a liquid chromatography-mass spectrometry instrument for detection. According to the method, a sample one-step method derivativeelution strategy is creatively invented in the elution stage of the vitamin D metabolite of the sample, so that the analysis sensitivity of the immune mass spectrometry is improved, the accurate quantification of the vitamin D metabolite is realized, and the additional sample treatment time and steps are not increased.

The invention discloses new pharmaceutical application of fructus ligustri lucidi and fructus ligustri lucidi extracts in preparing medicines for adjusting human body vitamin D3 metabolism and medicines for promoting calcium absorption, and also discloses granules and capsules for adjusting human body vitamin D3 metabolism and promoting calcium absorption; the medicines for adjusting human body vitamin D3 metabolism adopt ethanol extracts of dry and ripening fructus ligustri lucidi or fat-soluble components in the ethanol extracts; the medicines for promoting calcium absorption adopt water-soluble components in the ethanol extracts of the fructus ligustri lucidi. The application of the single medicines prepared by fructus ligustri lucidi in adjusting human body vitamin D3 metabolism and promoting calcium absorption and corresponding medicinal preparations have the advantages of curative effects, simple preparation process and relatively low cost.

Provided are methods of detecting the presence or amount of a dihydroxyvitamin D metabolite in a sample using mass spectrometry. The methods generally comprise associating an amine with a dihydroxyvitamin D metabolite in a sample, ionizing the adduct, and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample.

The invention provides a primer group for detection of vitamin E metabolism gene TTPA (tocopherol (Alpha) transfer protein) and a multiple PCR (polymerase chain reaction) detection method. The primer group includes at least two of five primer pairs for detecting first to five exons of vitamin E metabolism gene TTPA respectively; nucleotide sequences of upstream and downstream primers to the five primer pairs are shown as SEQ ID NO. 1 to 10 respectively. When applied to multiple PCR detection, the primer group is suitable for detecting gene mutations of at least two exons through a single reaction, so that the detection flux is increased.

The invention concerns a process for the production of a hybridoma, and of a monoclonal antibody or fragments thereof able to recognize 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3.

The invention discloses a pregnancy vitamin B12 deficiency risk assessment detection kit and an application method, and relates to molecular biology. A primer group for screening and predicting the deficiency risk of the vitamin B12 during pregnancy is used for amplifying single nucleotide polymorphism (SNP) sites of metabolic genes of the vitamin B12; and the length of the primers is 18-30 bp. The application method comprises the following steps of adding an SNP sequence specific extension primer into a product subjected to multiple PCR amplification, extending one basic group on an SNP site, extending different basic groups on different genotypes, exciting by using instantaneous nanosecond (10-9s) intense laser, separating in a non-electric field drift region according to the mass-to-charge ratio, wherein different genotypes are distinguished according to different time of flight to a detector in a vacuum tubule due to different masses of extension bases.

The invention discloses a Chinese traditional medicine and western medicine compound preparation for treating constipation and a preparation method thereof. The preparation comprises a Chinese traditional medicine component, a western medicine component and auxiliaries. The Chinese traditional medicine component is prepared from the following raw medicinal materials: camellia seeds, Chinese torreya, common perilla fruits, semen raphani, semen biotae, dessert almonds, plum kernels, wild semen juglandis, peach kernels, walnut kernels, fructus cannabis, snakegourd seeds, fructus amomi and fructus alpiniae oxyphyllae. The western medicine component is functional oligosaccharide. Aiming at cold constipation caused by deficiency of yang, endogenous coldness, stagnation of intestines and stomach, non-transportation of yang qi and non-distribution of body fluid, the Chinese traditional medicine and western medicine compound preparation disclosed by the invention has the effects of warming the kidney and assisting yang, warming spleen and stomach, tonifying kidney and securing essence and treating stranguria and activating blood so as to achieve the purpose of loosening the bowel to relieve constipation. In addition, the functional oligosaccharide selected can be used for improving vitamin metabolism so as to prevent bowel dysfunction and inhibit growth of harmful bacteria and pathogenic bacteria in the intestinal tract, thereby exerting the effects of improving the environment of the intestinal tract and protecting the intestinal tract. By combining Chinese traditional medicines which treat root causes and western medicines which regulate, the preparation disclosed by the invention fully exerts a curative effect and constipation cured is not easy to replase.

The invention provides a primer group, a kit and a method for detecting vitamin D metabolic gene mutation. The primer group comprises ten primer pairs of which the nucleotide sequences of upstream anddownstream primers are shown as SEQ ID NO.1-20 respectively. When the primer group is used for amplifying vitamin D metabolic genes; a plurality of exon and intron regions of the vitamin D metabolicgenes CYP24A1, CYP27B1 and VDR can be simultaneously amplified through one-time reaction, so that a plurality of high-frequency mutation sites of the vitamin D metabolic genes CYP24A1, CYP27B1 and VDRcan be simultaneously detected.

The invention relates to the technical field of gene detection, in particular to a typing kit, primers and a typing method for vitamin K metabolism related genes. The typing kit at least comprises a nucleic acid amplification reagent and a single-base extension reaction reagent, the nucleic acid amplification reagent contains PCR (Polymerase Chain Reaction) amplification primers as shown in SEQ ID NO: 1 to SEQ ID NO: 22; the single base extension reaction reagent contains single base extension primers as shown in SEQ ID NO: 23 to SEQ ID NO: 43. The typing method comprises the following steps: carrying out a PCR amplification reaction on the PCR amplification primer, carrying out alkaline phosphatase digestion, carrying out a single-base extension reaction on the single-base extension primer, carrying out desalination treatment, and carrying out high performance liquid chromatography-mass spectrometry detection. The kit and the detection method provided by the invention can improve the typing detection efficiency of the vitamin K metabolism related genes, and are simple, convenient, feasible, high in accuracy and high in automation degree.

The invention relates to the technical field of gene detection, and particularly discloses a single-base extension primer group and a method for detecting polymorphism of a vitamin A metabolism related gene. The single-base extension primer group at least comprises one of primers of which the nucleotide sequences are as shown in SEQ ID NO.1-15. The detection method comprises the following steps: respectively designing a multiple PCR amplification primer group and the single base extension primer group, carrying out PCR amplification by taking the multiple PCR amplification primer group as primers, and then carrying out an enzyme digestion reaction; then adding the single-base extension primer group to carry out a single-base extension reaction; and finally, purifying the single-base extension product, and carrying out mass spectrometric detection to obtain the polymorphism of the vitamin A related gene of the sample to be detected. The detection method provided by the invention has the advantage of high-throughput detection of gene polymorphic sites.

The invention relates to lanthanum hydroxide which has the functions of preparing and curing chronic renal failure (CRF), hyperphosphatemia or / and hyperparathyroidism (SHPT), vitamin D metabolic block, renal osteopathy and cardiovascular system calcification drugs, wherein the lanthanum hydroxide is nanometer lanthanum hydroxide, and daily dose of the lanthanum hydroxide or the nanometer lanthanum hydroxide is 600mg to 7800mg..

The invention provides a primer set for detecting a vitamin B12 metabolism gene mutation and an application method thereof. The primer set comprises at least two primer pairs in 22 primer pairs shownin SEQ ID NO.1 to SEQ ID NO.44. According to the primer set, the detection throughput of vitamin B12 metabolism gene detection can be improved.

The invention relates to the technical field of biology and provides a nutrition metabolic capacity related SNP site detection primer group, application, product and method. The nutrition metabolic capacity related SNP site detection primer group can be used for detecting vitamin A metabolic capacity, vitamin B6 metabolic capacity, vitamin B12 metabolic capacity, vitamin C metabolic capacity, vitamin D metabolic capacity, vitamin E metabolic capacity, elemental calcium metabolic capacity, elemental iron metabolic capacity, elemental zinc metabolic capacity, elemental selenium metabolic capacity, elemental iodine metabolic capacity, elemental sodium metabolic capacity, alcohol metabolic capacity, folic acid metabolic capacity, lactose metabolic capacity, unsaturated fatty acid metabolic capacity, saturated fatty acid metabolic capacity, cholesterol metabolic capacity, carbohydrate metabolic capacity and caffeine metabolic capacity. By the primer group, specific detection can be realized, high accuracy is achieved, a detection period can be shortened sharply, and detection cost is reduced.

The invention relates to the technical field of analysis and detection, in particular to a combined detection method for multiple vitamin D metabolites and a detection kit and application thereof. According to the method for detecting the vitamin D metabolites, liquid chromatography-tandem mass spectrometry is adopted for detection, a mobile phase of liquid chromatography comprises a mobile phase A and a mobile phase B, the mobile phase A is an aqueous solution containing lithium acetate, and the mobile phase B is a methanol solution containing lithium acetate. According to the method, the simultaneous detection of 25OHD3, 25OHD2, 1, 25 (OH) 2D3, 1, 25 (OH) 2D2, 24, 25 (OH) 2D3 and 24, 25 (OH) 2D2 can be realized, and various isomers and interferences can be distinguished at the same time; and the method has high sensitivity and specificity, does not need derivatization treatment, saves sample pretreatment time, and provides convenience for high-throughput detection of vitamin D metabolites.

The invention provides a method for detecting a vitamin D metabolite. The method comprises the following steps that: an analyte, namely the vitamin D metabolite, in a serum sample, is extracted by using an organic solvent; and a derivative reagent 4-phenylurazole and iodobenzene diacetate are added into a blow-dried extraction residue, so that Diels-Alder reaction can be performed. According to the method, the vitamin D metabolite is detected by using a liquid chromatography-tandem mass spectrometry method. The method has the advantages of low cost, short reaction time, high sensitivity and high interference resistance.

The invention discloses a gene polymorphism detection kit for a vitamin D metabolism marker and a detection method and application thereof. The marker for vitamin D metabolism is the polymorphism of two genes VDR and VDR, and the kit designs a specific amplification primer and a sequencing primer aiming at the polymorphism of two genes VDR FokI and VDR BsmI. The kit comprises the following components: an amplification reaction solution, a VDR FokI sequencing primer, a VDR BsmI sequencing primer and a positive control. The gene polymorphism related to calcium absorption and utilization capacity detection is detected by combining blood direct expansion, rapid amplification and optimized pyrosequencing technologies, and suggestions from the gene perspective are provided for clinical use of calcium absorption and utilization.

The invention discloses wine with alcohol effects capable of being fast dispelled. The wine is prepared from the following raw materials in proportion by weight: 500g of radix salviae miltiorrhizae, 300g of fruits of Chinese wolfberry, 500g of rose flowers, 300g of fructus chebulae immaturus and 300g of distiller's yeast; the distiller's yeast is prepared from the following raw materials in partsby weight: 80 parts of ganoderma spores, 50 parts of wheat, 50 parts of barley and 50 parts of pea. The invention also discloses a preparation method of the wine with alcohol effects capable of beingfast dispelled. The wine with alcohol effects capable of being fast dispelled prepared by the method has the functions of wetting and nourishing the livers, restoring the liver cell functions, activating the liver cell activity, assisting the liver function, synthetic protein metabolism and vitamin metabolism, regulating water-electrolyte balance and promoting bile secretion; the effects of oxidation, reduction, decomposition, combination and deoxygenation are achieved; some toxic substances are promoted to be fast discharged; the in-liver phagocyte effects are assisted; the liver toxic substances are cleared; the effects of improving the whole body immunocompetence, improving the whole body micro circulation function, relaxing tendons and activating blood circulation are achieved.

The present invention provides methods, devices, and compositions to rapidly detect analytes, including small analytes, using a lateral flow device. Described herein is such a lateral flow device that can detect and quantify vitamin D in a whole blood, serum, or plasma sample by employing a sandwich-based immunoassay.

The present invention provides a method of increasing or maintaining blood concentrations of 1,25-dihydroxyvitamin D in a patient by administering an amount of 1α-hydroxyvitamin D2. The invention also provides a method of concurrently lowering or maintaining plasma intact parathyroid hormone levels, increasing or maintaining serum calcium levels, maintaining serum phosphorous levels, or increasing or maintaining serum 1,25-dihydroxyvitamin D levels in a human patient by administering to the patient an amount of 1α-hydroxyvitamin D2. The invention also provides a method of reducing the risk of over suppression of plasma intact parathyroid hormone levels in a patient undergoing treatment for elevated levels of plasma intact parathyroid hormone, by administering 1α-hydroxyvitamin D2 in an amount sufficient to decrease elevated intact parathyroid hormone levels while avoiding an abnormally low bone turnover rate.